##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI AviII BbrPI
BcgI BciVI BclI BfrI BglII
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI DrdI EagI
EclXI Eco52I EcoO65I FbaI FseI
FspI HpaI MamI MfeI MroI
MunI NdeI NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
SalI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 4314 GGTACC G^GTACC
Alw44I 4344 GTGCAC G^TGCAC
Aor51HI 1413 AGCGCT AGC^GCT
ApaLI 4344 GTGCAC G^TGCAC
Asp700I 3111 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4314 GGTACC G^GTACC
AspI 3256 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3594 CCTAGG C^CTAGG
BamHI 621 GGATCC G^GATCC
BbeI 1055 GGCGCC GGCGC^C
BlnI 3594 CCTAGG C^CTAGG
Bsp1407I 1958 TGTACA T^GTACA
BspLU11I 3409 ACATGT A^CATGT
BsrFI 1856 GCCGGC [GA]^CCGG[CT]
BsrGI 1958 TGTACA T^GTACA
Cfr10I 1856 GCCGGC [GA]^CCGG[CT]
CpoI 4149 CGGACCG CG^G[AT]CCG
DraI 3317 TTTAAA TTT^AAA
DraIII 592 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1413 AGCGCT AGC^GCT
EcoRV 133 GATATC GAT^ATC
EcoT22I 3776 ATGCAT ATGCA^T
HindIII 1869 AAGCTT A^AGCTT
KasI 1055 GGCGCC G^GCGCC
KpnI 4314 GGTACC GGTAC^C
KspI 2615 CCGCGG CCGC^GG
MluI 3841 ACGCGT A^CGCGT
NaeI 1856 GCCGGC GCC^GGC
NarI 1055 GGCGCC GG^CGCC
NgoMIV 1856 GCCGGC G^CCGGC
NheI 2211 GCTAGC G^CTAGC
NsiI 3776 ATGCAT ATGCA^T
PaeR7I 352 CTCGAG C^TCGAG
PflFI 3256 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RsrII 4149 CGGACCG CG^G[AT]CCG
SacII 2615 CCGCGG CCGC^GG
SapI 3372 GCTCTTC GCTCTTC(1/4)
SbfI 1421 CCTGCAGG CCTGCA^GG
ScaI 3147 AGTACT AGT^ACT
SfoI 1055 GGCGCC GGC^GCC
SmaI 2515 CCCGGG CCC^GGG
SphI 2683 GCATGC GCATG^C
Sse8387I 1421 CCTGCAGG CCTGCA^GG
SspBI 1958 TGTACA T^GTACA
Tth111I 3256 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4016 TCTAGA T^CTAGA
XhoI 352 CTCGAG C^TCGAG
XmaCI 2515 CCCGGG C^CCGGG
XmaI 2515 CCCGGG C^CCGGG
XmnI 3111 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 2616 CGCG CG^CG
3842 CGCG
AcyI 1055 GGCGCC G[GA]^CG[CT]C
4248 GACGCC
AflIII 3409 ACATGT A^C[GA][CT]GT
3841 ACGCGT
AhdI 282 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
496 GACATGCTGTC
AspEI 282 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
496 GACATGCTGTC
BsaHI 1055 GGCGCC G[GA]^CG[CT]C
4248 GACGCC
BsmI 2832 GAATGC GAATGC(1/-1)
3533 GAATGC
BsrBI 788 CCGCTC CCGCTC(-3/-3)
1624 CCGCTC
BstUI 2616 CGCG CG^CG
3842 CGCG
Eam1105I 282 GACCCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
496 GACATGCTGTC
EcoRI 845 GAATTC G^AATTC
4012 GAATTC
Hin1I 1055 GGCGCC G[GA]^CG[CT]C
4248 GACGCC
HincII 408 GTCAAC GT[CT]^[GA]AC
830 GTCAAC
HindII 408 GTCAAC GT[CT]^[GA]AC
830 GTCAAC
MaeII 821 ACGT A^CGT
3753 ACGT
MslI 287 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4232 CATGTCTATG
MvnI 2616 CGCG CG^CG
3842 CGCG
PflMI 864 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2568 CCACGCTCTGG
SacI 1298 GAGCTC GAGCT^C
1631 GAGCTC
SexAI 412 ACCTGGT A^CC[AT]GGT
593 ACCTGGT
Van91I 864 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2568 CCACGCTCTGG
XcmI 75 CCAACCCGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1716 CCACCTGCAGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII