################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspI AviII AvrII BbeI BcgI BclI BcnI BfrI BglI BlnI BsaHI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco81I EcoO65I EcoRV FbaI FseI FspI Hin1I HindIII KasI KpnI KspI MfeI MroI MunI NarI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SmaI SpeI Sse8387I SspBI SwaI Tth111I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AhdI 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1903 GGGCCC GGGCC^C AspEI 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbrPI 1844 CACGTG CAC^GTG BsaBI 582 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 788 CGTCTC CGTCTC(1/5) BsmI 2896 GCATTC GAATGC(1/-1) BspLU11I 3390 ACATGT A^CATGT BssHII 1645 GCGCGC G^CGCGC BstAPI 156 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1024 CCAGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1145 GAATTC G^AATTC EcoT22I 2916 ATGCAT ATGCA^T HpaI 1745 GTTAAC GTT^AAC MamI 582 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluI 785 ACGCGT A^CGCGT NsiI 2916 ATGCAT ATGCA^T PmaCI 1844 CACGTG CAC^GTG PmlI 1844 CACGTG CAC^GTG PpuMI 1358 GGGACCT [GA]G^G[AT]CC[CT] PshAI 3633 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SgrAI 179 CGCCGGCG C[GA]^CCGG[CT]G SmlI 602 CTTGAG C^T[CT][GA]AG StuI 2555 AGGCCT AGG^CCT TaqI 3183 TCGA T^CGA TthHB8I 3183 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AflIII 785 ACGCGT A^C[GA][CT]GT 3390 ACATGT Aor51HI 71 AGCGCT AGC^GCT 3201 AGCGCT AspHI 269 GAGCAC G[AT]GC[AT]^C 2042 GTGCTC AvaI 291 CTCGGG C^[CT]CG[GA]G 3307 CCCGAG BalI 2550 TGGCCA TGG^CCA 2905 TGGCCA BamHI 587 GGATCC G^GATCC 3885 GGATCC BanI 1692 GGCACC G^G[CT][GA]CC 3848 GGTGCC BciVI 2505 GGATAC GTATCC(6/5) 2069 GTATCC BlpI 554 GCTAAGC GC^T[ACGT]AGC 3137 GCTGAGC Bpu1102I 554 GCTAAGC GC^T[ACGT]AGC 3137 GCTGAGC BsiHKAI 269 GAGCAC G[AT]GC[AT]^C 2042 GTGCTC BsoBI 291 CTCGGG C^[CT]CG[GA]G 3307 CCCGAG BspHI 804 TCATGA T^CATGA 2022 TCATGA BsrDI 1183 CATTGC GCAATG(2/0) 1481 CATTGC CelII 554 GCTAAGC GC^T[ACGT]AGC 3137 GCTGAGC DraII 1358 GGGACCT [GA]G^G[ACGT]CC[CT] 1902 AGGGCCC EagI 48 CGGCCG C^GGCCG 340 CGGCCG EclXI 48 CGGCCG C^GGCCG 340 CGGCCG Eco47III 71 AGCGCT AGC^GCT 3201 AGCGCT Eco52I 48 CGGCCG C^GGCCG 340 CGGCCG EcoNI 2558 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3535 CCTCACTAAGG EcoO109I 1358 GGGACCT [GA]G^G[ACGT]CC[CT] 1902 AGGGCCC HaeII 71 AGCGCT [GA]GCGC^[CT] 3201 AGCGCT MluNI 2550 TGGCCA TGG^CCA 2905 TGGCCA MscI 2550 TGGCCA TGG^CCA 2905 TGGCCA NaeI 180 GCCGGC GCC^GGC 338 GCCGGC NgoMIV 180 GCCGGC G^CCGGC 338 GCCGGC PflMI 241 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3363 CCATTGAATGG RcaI 804 TCATGA T^CATGA 2022 TCATGA ScaI 2392 AGTACT AGT^ACT 3759 AGTACT SnaBI 261 TACGTA TAC^GTA 1298 TACGTA SphI 698 GCATGC GCATG^C 2062 GCATGC Van91I 241 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3363 CCATTGAATGG XbaI 3100 TCTAGA T^CTAGA 3351 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AvaII BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI