##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I AocI
ApaLI AscI AseI AsnI Asp718I
AspI AviII AvrII BbeI BcgI
BclI BcnI BfrI BglI BlnI
BsaHI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DraIII DrdI Eco81I
EcoO65I EcoRV FbaI FseI FspI
Hin1I HindIII KasI KpnI KspI
MfeI MroI MunI NarI NciI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfoI SfuI SmaI SpeI Sse8387I
SspBI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1903 GGGCCC GGGCC^C
AspEI 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1844 CACGTG CAC^GTG
BsaBI 582 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 788 CGTCTC CGTCTC(1/5)
BsmI 2896 GCATTC GAATGC(1/-1)
BspLU11I 3390 ACATGT A^CATGT
BssHII 1645 GCGCGC G^CGCGC
BstAPI 156 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1024 CCAGGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 445 GACGTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1145 GAATTC G^AATTC
EcoT22I 2916 ATGCAT ATGCA^T
HpaI 1745 GTTAAC GTT^AAC
MamI 582 GATGCGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 785 ACGCGT A^CGCGT
NsiI 2916 ATGCAT ATGCA^T
PmaCI 1844 CACGTG CAC^GTG
PmlI 1844 CACGTG CAC^GTG
PpuMI 1358 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3633 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SgrAI 179 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 602 CTTGAG C^T[CT][GA]AG
StuI 2555 AGGCCT AGG^CCT
TaqI 3183 TCGA T^CGA
TthHB8I 3183 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 785 ACGCGT A^C[GA][CT]GT
3390 ACATGT
Aor51HI 71 AGCGCT AGC^GCT
3201 AGCGCT
AspHI 269 GAGCAC G[AT]GC[AT]^C
2042 GTGCTC
AvaI 291 CTCGGG C^[CT]CG[GA]G
3307 CCCGAG
BalI 2550 TGGCCA TGG^CCA
2905 TGGCCA
BamHI 587 GGATCC G^GATCC
3885 GGATCC
BanI 1692 GGCACC G^G[CT][GA]CC
3848 GGTGCC
BciVI 2505 GGATAC GTATCC(6/5)
2069 GTATCC
BlpI 554 GCTAAGC GC^T[ACGT]AGC
3137 GCTGAGC
Bpu1102I 554 GCTAAGC GC^T[ACGT]AGC
3137 GCTGAGC
BsiHKAI 269 GAGCAC G[AT]GC[AT]^C
2042 GTGCTC
BsoBI 291 CTCGGG C^[CT]CG[GA]G
3307 CCCGAG
BspHI 804 TCATGA T^CATGA
2022 TCATGA
BsrDI 1183 CATTGC GCAATG(2/0)
1481 CATTGC
CelII 554 GCTAAGC GC^T[ACGT]AGC
3137 GCTGAGC
DraII 1358 GGGACCT [GA]G^G[ACGT]CC[CT]
1902 AGGGCCC
EagI 48 CGGCCG C^GGCCG
340 CGGCCG
EclXI 48 CGGCCG C^GGCCG
340 CGGCCG
Eco47III 71 AGCGCT AGC^GCT
3201 AGCGCT
Eco52I 48 CGGCCG C^GGCCG
340 CGGCCG
EcoNI 2558 CCTAAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3535 CCTCACTAAGG
EcoO109I 1358 GGGACCT [GA]G^G[ACGT]CC[CT]
1902 AGGGCCC
HaeII 71 AGCGCT [GA]GCGC^[CT]
3201 AGCGCT
MluNI 2550 TGGCCA TGG^CCA
2905 TGGCCA
MscI 2550 TGGCCA TGG^CCA
2905 TGGCCA
NaeI 180 GCCGGC GCC^GGC
338 GCCGGC
NgoMIV 180 GCCGGC G^CCGGC
338 GCCGGC
PflMI 241 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3363 CCATTGAATGG
RcaI 804 TCATGA T^CATGA
2022 TCATGA
ScaI 2392 AGTACT AGT^ACT
3759 AGTACT
SnaBI 261 TACGTA TAC^GTA
1298 TACGTA
SphI 698 GCATGC GCATG^C
2062 GCATGC
Van91I 241 CCATTGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3363 CCATTGAATGG
XbaI 3100 TCTAGA T^CTAGA
3351 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaII
BanII BbsI BbvCI BbvI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NcoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII XmnI