################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccI AflII AscI AseI AsnI Asp700I AviII AvrII BamHI BbeI BfrI BlnI BsaBI BsaI BsiWI BspDI BspHI BspLU11I BssHII BssSI Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DraIII EcoO65I EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI PacI PmeI PshAI PshBI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SnaBI SpeI Sse8387I StuI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 3184 GGTACC G^GTACC AccIII 2804 TCCGGA T^CCGGA AclI 1171 AACGTT AA^CGTT AgeI 3795 ACCGGT A^CCGGT AhdI 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2068 CCTGAGG CC^T[ACGT]AGG Aor51HI 1122 AGCGCT AGC^GCT ApaI 588 GGGCCC GGGCC^C Asp718I 3184 GGTACC G^GTACC AspEI 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 2139 TGGCCA TGG^CCA BbvCI 1393 CCTCAGC CCTCAGC(-5/-2) BcgI 2636 GCAGTATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 893 TGATCA T^GATCA BlpI 789 GCTCAGC GC^T[ACGT]AGC Bpu1102I 789 GCTCAGC GC^T[ACGT]AGC BseAI 2804 TCCGGA T^CCGGA BspEI 2804 TCCGGA T^CCGGA BsrDI 1541 GCAATG GCAATG(2/0) BstBI 1953 TTCGAA TT^CGAA Bsu36I 2068 CCTGAGG CC^T[ACGT]AGG CelII 789 GCTCAGC GC^T[ACGT]AGC EagI 800 CGGCCG C^GGCCG Eam1105I 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 800 CGGCCG C^GGCCG Eco47III 1122 AGCGCT AGC^GCT Eco52I 800 CGGCCG C^GGCCG Eco81I 2068 CCTGAGG CC^T[ACGT]AGG EcoRI 2155 GAATTC G^AATTC FbaI 893 TGATCA T^GATCA HaeII 1122 AGCGCT [GA]GCGC^[CT] KpnI 3184 GGTACC GGTAC^C MluNI 2139 TGGCCA TGG^CCA MroI 2804 TCCGGA T^CCGGA MscI 2139 TGGCCA TGG^CCA NaeI 1193 GCCGGC GCC^GGC NgoMIV 1193 GCCGGC G^CCGGC NspV 1953 TTCGAA TT^CGAA PaeR7I 1746 CTCGAG C^TCGAG PinAI 3795 ACCGGT A^CCGGT Psp1406I 1171 AACGTT AA^CGTT SacI 49 GAGCTC GAGCT^C SfuI 1953 TTCGAA TT^CGAA SgrAI 99 CACCGGCG C[GA]^CCGG[CT]G SmlI 1746 CTCGAG C^T[CT][GA]AG SspI 841 AATATT AAT^ATT XhoI 1746 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 91 GACGTC GACGT^C 1282 GACGTC AcsI 2155 GAATTC [GA]^AATT[CT] 3592 AAATTT Alw44I 712 GTGCAC G^TGCAC 3288 GTGCAC AlwNI 2722 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 2976 CAGGCACTG ApaLI 712 GTGCAC G^TGCAC 3288 GTGCAC ApoI 2155 GAATTC [GA]^AATT[CT] 3592 AAATTT BbrPI 3100 CACGTG CAC^GTG 3661 CACGTG BglI 985 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3345 GCCTTGCTGGC BglII 1200 AGATCT A^GATCT 3878 AGATCT BsmI 444 GAATGC GAATGC(1/-1) 2084 GAATGC BsrBI 394 GAGCGG CCGCTC(-3/-3) 1090 GAGCGG DraI 1378 TTTAAA TTT^AAA 3820 TTTAAA EcoNI 618 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2285 CCTCCAGGAGG HgaI 107 GCGTC GACGC(5/10) 598 GCGTC HincII 94 GTCAAC GT[CT]^[GA]AC 2889 GTTGAC HindII 94 GTCAAC GT[CT]^[GA]AC 2889 GTTGAC MslI 667 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 3208 CACTGACATG NcoI 806 CCATGG C^CATGG 2573 CCATGG PflMI 505 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3328 CCAAGAGTTGG PmaCI 3100 CACGTG CAC^GTG 3661 CACGTG PmlI 3100 CACGTG CAC^GTG 3661 CACGTG PpuMI 500 GGGTCCC [GA]G^G[AT]CC[CT] 2044 GGGACCC PstI 247 CTGCAG CTGCA^G 1063 CTGCAG SmaI 481 CCCGGG CCC^GGG 1917 CCCGGG SphI 1490 GCATGC GCATG^C 1553 GCATGC Van91I 505 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3328 CCAAGAGTTGG XcmI 572 CCAATGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3076 CCATACTTCCCTTGG XmaCI 481 CCCGGG C^CCGGG 1917 CCCGGG XmaI 481 CCCGGG C^CCGGG 1917 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AspHI AspI AvaI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DrdI DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflFI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII