##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AflII AscI AseI AsnI
Asp700I AviII AvrII BamHI BbeI
BfrI BlnI BsaBI BsaI BsiWI
BspDI BspHI BspLU11I BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI DraIII EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KspI MamI MfeI MluI
MunI NarI NdeI NheI NotI
NruI NsiI PacI PmeI PshAI
PshBI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SnaBI SpeI Sse8387I
StuI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3184 GGTACC G^GTACC
AccIII 2804 TCCGGA T^CCGGA
AclI 1171 AACGTT AA^CGTT
AgeI 3795 ACCGGT A^CCGGT
AhdI 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2068 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1122 AGCGCT AGC^GCT
ApaI 588 GGGCCC GGGCC^C
Asp718I 3184 GGTACC G^GTACC
AspEI 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2139 TGGCCA TGG^CCA
BbvCI 1393 CCTCAGC CCTCAGC(-5/-2)
BcgI 2636 GCAGTATTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 893 TGATCA T^GATCA
BlpI 789 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 789 GCTCAGC GC^T[ACGT]AGC
BseAI 2804 TCCGGA T^CCGGA
BspEI 2804 TCCGGA T^CCGGA
BsrDI 1541 GCAATG GCAATG(2/0)
BstBI 1953 TTCGAA TT^CGAA
Bsu36I 2068 CCTGAGG CC^T[ACGT]AGG
CelII 789 GCTCAGC GC^T[ACGT]AGC
EagI 800 CGGCCG C^GGCCG
Eam1105I 3012 GACTGAACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 800 CGGCCG C^GGCCG
Eco47III 1122 AGCGCT AGC^GCT
Eco52I 800 CGGCCG C^GGCCG
Eco81I 2068 CCTGAGG CC^T[ACGT]AGG
EcoRI 2155 GAATTC G^AATTC
FbaI 893 TGATCA T^GATCA
HaeII 1122 AGCGCT [GA]GCGC^[CT]
KpnI 3184 GGTACC GGTAC^C
MluNI 2139 TGGCCA TGG^CCA
MroI 2804 TCCGGA T^CCGGA
MscI 2139 TGGCCA TGG^CCA
NaeI 1193 GCCGGC GCC^GGC
NgoMIV 1193 GCCGGC G^CCGGC
NspV 1953 TTCGAA TT^CGAA
PaeR7I 1746 CTCGAG C^TCGAG
PinAI 3795 ACCGGT A^CCGGT
Psp1406I 1171 AACGTT AA^CGTT
SacI 49 GAGCTC GAGCT^C
SfuI 1953 TTCGAA TT^CGAA
SgrAI 99 CACCGGCG C[GA]^CCGG[CT]G
SmlI 1746 CTCGAG C^T[CT][GA]AG
SspI 841 AATATT AAT^ATT
XhoI 1746 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 91 GACGTC GACGT^C
1282 GACGTC
AcsI 2155 GAATTC [GA]^AATT[CT]
3592 AAATTT
Alw44I 712 GTGCAC G^TGCAC
3288 GTGCAC
AlwNI 2722 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2976 CAGGCACTG
ApaLI 712 GTGCAC G^TGCAC
3288 GTGCAC
ApoI 2155 GAATTC [GA]^AATT[CT]
3592 AAATTT
BbrPI 3100 CACGTG CAC^GTG
3661 CACGTG
BglI 985 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3345 GCCTTGCTGGC
BglII 1200 AGATCT A^GATCT
3878 AGATCT
BsmI 444 GAATGC GAATGC(1/-1)
2084 GAATGC
BsrBI 394 GAGCGG CCGCTC(-3/-3)
1090 GAGCGG
DraI 1378 TTTAAA TTT^AAA
3820 TTTAAA
EcoNI 618 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2285 CCTCCAGGAGG
HgaI 107 GCGTC GACGC(5/10)
598 GCGTC
HincII 94 GTCAAC GT[CT]^[GA]AC
2889 GTTGAC
HindII 94 GTCAAC GT[CT]^[GA]AC
2889 GTTGAC
MslI 667 CACAGTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3208 CACTGACATG
NcoI 806 CCATGG C^CATGG
2573 CCATGG
PflMI 505 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3328 CCAAGAGTTGG
PmaCI 3100 CACGTG CAC^GTG
3661 CACGTG
PmlI 3100 CACGTG CAC^GTG
3661 CACGTG
PpuMI 500 GGGTCCC [GA]G^G[AT]CC[CT]
2044 GGGACCC
PstI 247 CTGCAG CTGCA^G
1063 CTGCAG
SmaI 481 CCCGGG CCC^GGG
1917 CCCGGG
SphI 1490 GCATGC GCATG^C
1553 GCATGC
Van91I 505 CCACCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3328 CCAAGAGTTGG
XcmI 572 CCAATGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3076 CCATACTTCCCTTGG
XmaCI 481 CCCGGG C^CCGGG
1917 CCCGGG
XmaI 481 CCCGGG C^CCGGG
1917 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AspHI AspI AvaI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I XhoII