##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
BbeI BbrPI BcgI BfrI BglI
BsaAI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoRV FseI FspI
HindIII KasI KpnI KspI MfeI
MluI MroI MunI NarI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 3515 GACGTC GACGT^C
AccII 1736 CGCG CG^CG
AflIII 3650 ACGTGT A^C[GA][CT]GT
AgeI 782 ACCGGT A^CCGGT
Aor51HI 2690 AGCGCT AGC^GCT
ApaI 2590 GGGCCC GGGCC^C
BbvCI 2191 GCTGAGG CCTCAGC(-5/-2)
BciVI 13 GGATAC GTATCC(6/5)
BclI 3370 TGATCA T^GATCA
BglII 1994 AGATCT A^GATCT
BsaBI 567 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BstUI 1736 CGCG CG^CG
Eco47III 2690 AGCGCT AGC^GCT
EcoT22I 2212 ATGCAT ATGCA^T
FbaI 3370 TGATCA T^GATCA
HpaI 4009 GTTAAC GTT^AAC
MamI 567 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1736 CGCG CG^CG
NcoI 1608 CCATGG C^CATGG
NheI 2139 GCTAGC G^CTAGC
NsiI 2212 ATGCAT ATGCA^T
PflMI 2468 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 782 ACCGGT A^CCGGT
SexAI 3640 ACCTGGT A^CC[AT]GGT
SmaI 182 CCCGGG CCC^GGG
Van91I 2468 CCAAGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2833 TCTAGA T^CTAGA
XmaCI 182 CCCGGG C^CCGGG
XmaI 182 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 2656 GAATTC [GA]^AATT[CT]
2837 GAATTC
AcyI 2550 GACGCC G[GA]^CG[CT]C
3515 GACGTC
AocI 8 CCTGAGG CC^T[ACGT]AGG
3291 CCTCAGG
ApoI 2656 GAATTC [GA]^AATT[CT]
2837 GAATTC
Asp700I 477 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3211 GAAGTTTTTC
AvaI 182 CCCGGG C^[CT]CG[GA]G
1628 CCCGAG
AvrII 47 CCTAGG C^CTAGG
3117 CCTAGG
BalI 621 TGGCCA TGG^CCA
3925 TGGCCA
BamHI 1755 GGATCC G^GATCC
3430 GGATCC
BbsI 1525 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BlnI 47 CCTAGG C^CTAGG
3117 CCTAGG
BpuAI 1525 GAAGAC GAAGAC(2/6)
2107 GTCTTC
BsaHI 2550 GACGCC G[GA]^CG[CT]C
3515 GACGTC
BsaI 3637 GAGACC GGTCTC(1/5)
3199 GGTCTC
BsmI 3551 GAATGC GAATGC(1/-1)
152 GCATTC
BsoBI 182 CCCGGG C^[CT]CG[GA]G
1628 CCCGAG
BspMI 226 ACCTGC ACCTGC(4/8)
3544 ACCTGC
BsrBI 1317 CCGCTC CCGCTC(-3/-3)
1335 CCGCTC
BsrDI 2734 CATTGC GCAATG(2/0)
3179 GCAATG
BssSI 147 CACGAG CACGAG(-5/-1)
3306 CTCGTG
BstAPI 464 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3759 GCAGCTGGTGC
BstEII 501 GGTGACC G^GT[ACGT]ACC
2019 GGTGACC
BstPI 501 GGTGACC G^GT[ACGT]ACC
2019 GGTGACC
BstXI 588 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2905 CCACCTCAGTGG
Bsu36I 8 CCTGAGG CC^T[ACGT]AGG
3291 CCTCAGG
DraI 81 TTTAAA TTT^AAA
3455 TTTAAA
Eco81I 8 CCTGAGG CC^T[ACGT]AGG
3291 CCTCAGG
EcoNI 2082 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2516 CCTGCTTCAGG
EcoO65I 501 GGTGACC G^GT[ACGT]ACC
2019 GGTGACC
EcoRI 2656 GAATTC G^AATTC
2837 GAATTC
HaeII 2075 AGCGCC [GA]GCGC^[CT]
2690 AGCGCT
HgaI 2550 GACGC GACGC(5/10)
686 GCGTC
Hin1I 2550 GACGCC G[GA]^CG[CT]C
3515 GACGTC
HincII 1969 GTCAAC GT[CT]^[GA]AC
4009 GTTAAC
HindII 1969 GTCAAC GT[CT]^[GA]AC
4009 GTTAAC
MluNI 621 TGGCCA TGG^CCA
3925 TGGCCA
MscI 621 TGGCCA TGG^CCA
3925 TGGCCA
NaeI 1706 GCCGGC GCC^GGC
2553 GCCGGC
NgoMIV 1706 GCCGGC G^CCGGC
2553 GCCGGC
TaqI 2132 TCGA T^CGA
2340 TCGA
TthHB8I 2132 TCGA T^CGA
2340 TCGA
XmnI 477 GAAGCCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3211 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII