##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AgeI Aor51HI AscI Asp700I BbrPI
BbvCI BclI BfrI BsaBI BseAI
BsmBI BsmI BspDI BspEI BspHI
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI Eco47III EcoRV EcoT22I FbaI
MamI MluI MroI NruI NsiI
PacI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SgrAI SnaBI Sse8387I SspI StuI
SwaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 193 GGTACC G^GTACC
AhdI 4855 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1883 GTGCAC G^TGCAC
AocI 1117 CCTGAGG CC^T[ACGT]AGG
ApaLI 1883 GTGCAC G^TGCAC
AseI 4965 ATTAAT AT^TAAT
AsnI 4965 ATTAAT AT^TAAT
Asp718I 193 GGTACC G^GTACC
AspEI 4855 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3693 TGCGCA TGC^GCA
AvrII 3558 CCTAGG C^CTAGG
BbeI 317 GGCGCC GGCGC^C
BcgI 3362 CGACTCACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1748 GGATAC GTATCC(6/5)
BlnI 3558 CCTAGG C^CTAGG
BsaAI 1233 TACGTG [CT]AC^GT[GA]
BsaWI 583 TCCGGT [AT]^CCGG[AT]
BsiWI 1827 CGTACG C^GTACG
Bsp1407I 2702 TGTACA T^GTACA
BsrGI 2702 TGTACA T^GTACA
BstBI 1142 TTCGAA TT^CGAA
BstXI 3598 CCACTGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1117 CCTGAGG CC^T[ACGT]AGG
DraI 4299 TTTAAA TTT^AAA
DrdI 3027 GACCTGGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4855 GACACAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1117 CCTGAGG CC^T[ACGT]AGG
FseI 4392 GGCCGGCC GGCCGG^CC
FspI 3693 TGCGCA TGC^GCA
HpaI 2435 GTTAAC GTT^AAC
KasI 317 GGCGCC G^GCGCC
KpnI 193 GGTACC GGTAC^C
KspI 119 CCGCGG CCGC^GG
MfeI 2612 CAATTG C^AATTG
MunI 2612 CAATTG C^AATTG
NarI 317 GGCGCC GG^CGCC
NheI 3628 GCTAGC G^CTAGC
NotI 33 GCGGCCGC GC^GGCCGC
NspV 1142 TTCGAA TT^CGAA
PaeR7I 536 CTCGAG C^TCGAG
PshBI 4965 ATTAAT AT^TAAT
SacII 119 CCGCGG CCGC^GG
SfoI 317 GGCGCC GGC^GCC
SfuI 1142 TTCGAA TT^CGAA
SmaI 322 CCCGGG CCC^GGG
SpeI 3987 ACTAGT A^CTAGT
SspBI 2702 TGTACA T^GTACA
XbaI 4950 TCTAGA T^CTAGA
XhoI 536 CTCGAG C^TCGAG
XmaCI 322 CCCGGG C^CCGGG
XmaI 322 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 261 GACGCC G[GA]^CG[CT]C
317 GGCGCC
ApaI 334 GGGCCC GGGCC^C
3534 GGGCCC
AspI 2642 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4633 GACAGGGTC
BamHI 11 GGATCC G^GATCC
4511 GGATCC
BglII 415 AGATCT A^GATCT
4115 AGATCT
BsaHI 261 GACGCC G[GA]^CG[CT]C
317 GGCGCC
BsaI 1476 GAGACC GGTCTC(1/5)
2371 GGTCTC
BstEII 2768 GGTCACC G^GT[ACGT]ACC
3306 GGTGACC
BstPI 2768 GGTCACC G^GT[ACGT]ACC
3306 GGTGACC
DraIII 2378 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
4733 CACTGTGTG
EcoO65I 2768 GGTCACC G^GT[ACGT]ACC
3306 GGTGACC
EcoRI 4926 GAATTC G^AATTC
4981 GAATTC
Hin1I 261 GACGCC G[GA]^CG[CT]C
317 GGCGCC
HindIII 3387 AAGCTT A^AGCTT
3923 AAGCTT
NdeI 1274 CATATG CA^TATG
4901 CATATG
PflFI 2642 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4633 GACAGGGTC
SacI 686 GAGCTC GAGCT^C
2083 GAGCTC
SphI 1132 GCATGC GCATG^C
2292 GCATGC
Tth111I 2642 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4633 GACAGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BspMI BsrBI BsrDI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII