##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccI AccII AccIII AgeI
Aor51HI ApaI AscI Asp700I AspI
BbeI BglI BsaBI BsaWI BseAI
BsiEI BsiWI BspDI BspEI BspLU11I
BsrBI BsrFI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstZ17I Cfr10I
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRI EcoRV
FseI KasI KspI MamI MfeI
MluI MroI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PaeR7I PflFI PinAI
PmeI PvuI PvuII RsrII SacII
SalI ScaI SfiI SfoI SgrAI
SnaBI SphI Tth111I XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AclI 1258 AACGTT AA^CGTT
AcyI 3565 GACGCC G[GA]^CG[CT]C
AocI 4388 CCTTAGG CC^T[ACGT]AGG
AviII 924 TGCGCA TGC^GCA
AvrII 2936 CCTAGG C^CTAGG
BamHI 100 GGATCC G^GATCC
BbrPI 2655 CACGTG CAC^GTG
BclI 1349 TGATCA T^GATCA
BlnI 2936 CCTAGG C^CTAGG
BlpI 3699 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3699 GCTCAGC GC^T[ACGT]AGC
BsaHI 3565 GACGCC G[GA]^CG[CT]C
BsmBI 3563 GAGACG CGTCTC(1/5)
Bsp1407I 3501 TGTACA T^GTACA
BsrDI 6 GCAATG GCAATG(2/0)
BsrGI 3501 TGTACA T^GTACA
BstBI 1568 TTCGAA TT^CGAA
Bsu36I 4388 CCTTAGG CC^T[ACGT]AGG
CelII 3699 GCTCAGC GC^T[ACGT]AGC
Eco81I 4388 CCTTAGG CC^T[ACGT]AGG
EcoNI 184 CCTGCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 336 ATGCAT ATGCA^T
FauI 1816 GCGGG CCCGC(4/6)
FbaI 1349 TGATCA T^GATCA
FspI 924 TGCGCA TGC^GCA
HgaI 3565 GACGC GACGC(5/10)
Hin1I 3565 GACGCC G[GA]^CG[CT]C
HpaI 4318 GTTAAC GTT^AAC
NsiI 336 ATGCAT ATGCA^T
NspV 1568 TTCGAA TT^CGAA
PacI 3111 TTAATTAA TTAAT^TAA
PmaCI 2655 CACGTG CAC^GTG
PmlI 2655 CACGTG CAC^GTG
PshAI 3988 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1258 AACGTT AA^CGTT
SacI 2760 GAGCTC GAGCT^C
SbfI 1910 CCTGCAGG CCTGCA^GG
SfuI 1568 TTCGAA TT^CGAA
SmaI 1951 CCCGGG CCC^GGG
SpeI 2110 ACTAGT A^CTAGT
Sse8387I 1910 CCTGCAGG CCTGCA^GG
SspBI 3501 TGTACA T^GTACA
StuI 710 AGGCCT AGG^CCT
SwaI 3524 ATTTAAAT ATTT^AAAT
XmaCI 1951 CCCGGG C^CCGGG
XmaI 1951 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AflII 2593 CTTAAG C^TTAAG
4098 CTTAAG
AflIII 3965 ACGTGT A^C[GA][CT]GT
4314 ACGTGT
AhdI 2161 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3623 GACCCCAAGTC
AseI 3517 ATTAAT AT^TAAT
4237 ATTAAT
AsnI 3517 ATTAAT AT^TAAT
4237 ATTAAT
AspEI 2161 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3623 GACCCCAAGTC
BbvCI 2354 GCTGAGG CCTCAGC(-5/-2)
2572 GCTGAGG
BcgI 1806 CGAACACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2381 GCAATCAAGTCG
BfrI 2593 CTTAAG C^TTAAG
4098 CTTAAG
BsaAI 2655 CACGTG [CT]AC^GT[GA]
4313 TACGTG
BsgI 3598 CTGCAC GTGCAG(16/14)
1987 GTGCAG
BspHI 512 TCATGA T^CATGA
1581 TCATGA
BspMI 183 ACCTGC ACCTGC(4/8)
4437 ACCTGC
BstAPI 1127 GCAATTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3714 GCACTGGCTGC
DraIII 2896 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4123 CACAATGTG
Eam1105I 2161 GACTCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3623 GACCCCAAGTC
HaeII 2415 GGCGCT [GA]GCGC^[CT]
4248 AGCGCC
HincII 602 GTCAAC GT[CT]^[GA]AC
4318 GTTAAC
HindII 602 GTCAAC GT[CT]^[GA]AC
4318 GTTAAC
NspI 334 ACATGC [GA]CATG^[CT]
452 GCATGT
PflMI 897 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2895 CCACACAGTGG
PshBI 3517 ATTAAT AT^TAAT
4237 ATTAAT
RcaI 512 TCATGA T^CATGA
1581 TCATGA
SapI 3322 GCTCTTC GCTCTTC(1/4)
3723 GCTCTTC
SexAI 86 ACCTGGT A^CC[AT]GGT
3952 ACCAGGT
Van91I 897 CCAGTTGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2895 CCACACAGTGG
XbaI 3241 TCTAGA T^CTAGA
3728 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII