##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AocI ApaI AscI Asp718I AviII
BbvCI BcgI BciVI BsaBI BsiWI
BspDI BspHI BspLU11I BstEII BstPI
Bsu36I ClaI CpoI DraIII Eco81I
EcoO65I EcoRV FseI FspI KpnI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NheI NotI NruI
PacI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SalI SbfI
ScaI SexAI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccI 1601 GTATAC GT^[AC][GT]AC
AccIII 1513 TCCGGA T^CCGGA
AflII 1334 CTTAAG C^TTAAG
AhdI 3038 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 460 AGCGCT AGC^GCT
AseI 2788 ATTAAT AT^TAAT
AsnI 2788 ATTAAT AT^TAAT
Asp700I 3130 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 3038 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 594 CTCGAG C^[CT]CG[GA]G
BamHI 1516 GGATCC G^GATCC
BbeI 248 GGCGCC GGCGC^C
BbrPI 426 CACGTG CAC^GTG
BfrI 1334 CTTAAG C^TTAAG
BsaAI 426 CACGTG [CT]AC^GT[GA]
BseAI 1513 TCCGGA T^CCGGA
BsmBI 2190 CGTCTC CGTCTC(1/5)
BsoBI 594 CTCGAG C^[CT]CG[GA]G
BspEI 1513 TCCGGA T^CCGGA
BsrDI 1637 CATTGC GCAATG(2/0)
BsrFI 2225 GCCGGT [GA]^CCGG[CT]
Bst1107I 1601 GTATAC GTA^TAC
BstAPI 2357 GCATTTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3137 TTCGAA TT^CGAA
BstXI 3875 CCAGATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1601 GTATAC GTA^TAC
Cfr10I 2225 GCCGGT [GA]^CCGG[CT]
DrdI 996 GACATTGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 254 CGGCCG C^GGCCG
Eam1105I 3038 GACTTGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 254 CGGCCG C^GGCCG
Eco47III 460 AGCGCT AGC^GCT
Eco52I 254 CGGCCG C^GGCCG
EcoRI 921 GAATTC G^AATTC
EcoT22I 4173 ATGCAT ATGCA^T
HpaI 3489 GTTAAC GTT^AAC
KasI 248 GGCGCC G^GCGCC
MroI 1513 TCCGGA T^CCGGA
NarI 248 GGCGCC GG^CGCC
NsiI 4173 ATGCAT ATGCA^T
NspV 3137 TTCGAA TT^CGAA
PaeR7I 594 CTCGAG C^TCGAG
PflFI 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2300 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 426 CACGTG CAC^GTG
PmlI 426 CACGTG CAC^GTG
PpuMI 393 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 2788 ATTAAT AT^TAAT
SapI 4145 GCTCTTC GCTCTTC(1/4)
SfoI 248 GGCGCC GGC^GCC
SfuI 3137 TTCGAA TT^CGAA
SphI 2469 GCATGC GCATG^C
SspI 2902 AATATT AAT^ATT
Tth111I 770 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2300 CCAGAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 594 CTCGAG C^TCGAG
XmnI 3130 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 248 GGCGCC G[GA]^CG[CT]C
530 GGCGTC
Alw44I 3605 GTGCAC G^TGCAC
4414 GTGCAC
ApaLI 3605 GTGCAC G^TGCAC
4414 GTGCAC
BbsI 1725 GTCTTC GAAGAC(2/6)
3952 GTCTTC
BglII 2531 AGATCT A^GATCT
3877 AGATCT
BlpI 47 GCTAAGC GC^T[ACGT]AGC
144 GCTGAGC
Bpu1102I 47 GCTAAGC GC^T[ACGT]AGC
144 GCTGAGC
BpuAI 1725 GTCTTC GAAGAC(2/6)
3952 GTCTTC
BsaHI 248 GGCGCC G[GA]^CG[CT]C
530 GGCGTC
BsgI 123 GTGCAG GTGCAG(16/14)
946 GTGCAG
BssHII 282 GCGCGC G^CGCGC
522 GCGCGC
BssSI 1434 CTCGTG CACGAG(-5/-1)
2123 CTCGTG
CelII 47 GCTAAGC GC^T[ACGT]AGC
144 GCTGAGC
Hin1I 248 GGCGCC G[GA]^CG[CT]C
530 GGCGTC
HindIII 1296 AAGCTT A^AGCTT
3173 AAGCTT
SacI 72 GAGCTC GAGCT^C
1549 GAGCTC
TaqI 595 TCGA T^CGA
3138 TCGA
TthHB8I 595 TCGA T^CGA
3138 TCGA
XbaI 1774 TCTAGA T^CTAGA
3047 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
AvrII BalI BanI BanII BbvI
BclI BcnI BfaI BglI BlnI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrBI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RsaI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XcmI XhoII