##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AscI AseI AsnI
Asp718I AspEI AspI AviII BbrPI
BciVI BclI BfrI BsaBI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrFI BssHII BssSI Bst1107I BstBI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MamI MluI MroI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SacII SalI
ScaI SfuI SgrAI SmaI SnaBI
SpeI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 616 GACGTC GACGT^C
AcsI 1392 AAATTT [GA]^AATT[CT]
Alw44I 625 GTGCAC G^TGCAC
Aor51HI 1055 AGCGCT AGC^GCT
ApaI 2966 GGGCCC GGGCC^C
ApaLI 625 GTGCAC G^TGCAC
ApoI 1392 AAATTT [GA]^AATT[CT]
Asp700I 1207 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 25 CTCGGG C^[CT]CG[GA]G
AvrII 155 CCTAGG C^CTAGG
BbeI 2117 GGCGCC GGCGC^C
BcgI 17 GCAAGACCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 155 CCTAGG C^CTAGG
BlpI 3443 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3443 GCTAAGC GC^T[ACGT]AGC
BsaAI 2279 CACGTA [CT]AC^GT[GA]
BsaWI 31 TCCGGT [AT]^CCGG[AT]
BsmI 1471 GCATTC GAATGC(1/-1)
BsoBI 25 CTCGGG C^[CT]CG[GA]G
BspHI 916 TCATGA T^CATGA
BspLU11I 1610 ACATGT A^CATGT
BsrBI 761 GAGCGG CCGCTC(-3/-3)
BstAPI 892 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1709 GGTGACC G^GT[ACGT]ACC
BstPI 1709 GGTGACC G^GT[ACGT]ACC
CelII 3443 GCTAAGC GC^T[ACGT]AGC
DraIII 2978 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 21 GACCCTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1055 AGCGCT AGC^GCT
EcoO65I 1709 GGTGACC G^GT[ACGT]ACC
KasI 2117 GGCGCC G^GCGCC
MfeI 3400 CAATTG C^AATTG
MunI 3400 CAATTG C^AATTG
NarI 2117 GGCGCC GG^CGCC
NcoI 367 CCATGG C^CATGG
PshAI 1637 GACTCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 497 CAGCTG CAG^CTG
RcaI 916 TCATGA T^CATGA
SacI 3453 GAGCTC GAGCT^C
SexAI 1355 ACCTGGT A^CC[AT]GGT
SfiI 365 GGCCATGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2117 GGCGCC GGC^GCC
SphI 660 GCATGC GCATG^C
XmnI 1207 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 56 CGCG CG^CG
2796 CGCG
AcyI 616 GACGTC G[GA]^CG[CT]C
2117 GGCGCC
AflIII 1610 ACATGT A^C[GA][CT]GT
2278 ACACGT
AocI 3204 CCTCAGG CC^T[ACGT]AGG
3363 CCTCAGG
BamHI 74 GGATCC G^GATCC
2470 GGATCC
BanI 1322 GGCACC G^G[CT][GA]CC
2117 GGCGCC
BbvCI 421 CCTCAGC CCTCAGC(-5/-2)
2779 CCTCAGC
BsaHI 616 GACGTC G[GA]^CG[CT]C
2117 GGCGCC
BsaI 435 GAGACC GGTCTC(1/5)
300 GGTCTC
BsrDI 1400 CATTGC GCAATG(2/0)
3119 GCAATG
BstUI 56 CGCG CG^CG
2796 CGCG
BstXI 666 CCATGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCAGCCCTGTGG
Bsu36I 3204 CCTCAGG CC^T[ACGT]AGG
3363 CCTCAGG
DsaI 367 CCATGG C^C[GA][CT]GG
3130 CCGTGG
Eco81I 3204 CCTCAGG CC^T[ACGT]AGG
3363 CCTCAGG
FauI 60 GCGGG CCCGC(4/6)
1103 GCGGG
HgaI 3417 GACGC GACGC(5/10)
3463 GACGC
Hin1I 616 GACGTC G[GA]^CG[CT]C
2117 GGCGCC
MseI 1676 TTAA T^TAA
1699 TTAA
MvnI 56 CGCG CG^CG
2796 CGCG
PpuMI 456 GGGACCT [GA]G^G[AT]CC[CT]
2665 AGGTCCT
SapI 794 GAAGAGC GCTCTTC(1/4)
2107 GAAGAGC
SbfI 898 CCTGCAGG CCTGCA^GG
1834 CCTGCAGG
Sse8387I 898 CCTGCAGG CCTGCA^GG
1834 CCTGCAGG
Tru9I 1676 TTAA T^TAA
1699 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BalI BanII BbsI
BbvI BcnI BfaI BglI BglII
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII