##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI Alw44I ApaI ApaLI
AscI AspEI AvaI AviII AvrII
BalI BbeI BbrPI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BsaBI BsaWI BseAI BsiEI BsmBI
BsoBI BspEI BsrFI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstZ17I Cfr10I CpoI DrdI EaeI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRV FseI FspI HaeIII KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PaeR7I PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
StuI SwaI XbaI XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 481 GGTACC G^GTACC
AccI 3515 GTCTAC GT^[AC][GT]AC
AcyI 150 GACGCC G[GA]^CG[CT]C
AflIII 34 ACATGT A^C[GA][CT]GT
Aor51HI 1825 AGCGCT AGC^GCT
Asp718I 481 GGTACC G^GTACC
AspI 651 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 203 GGATCC G^GATCC
BclI 1446 TGATCA T^GATCA
BcnI 1922 CCCGG CC^[GC]GG
BlpI 1472 GCTTAGC GC^T[ACGT]AGC
BpmI 54 CTGGAG CTGGAG(16/14)
Bpu1102I 1472 GCTTAGC GC^T[ACGT]AGC
BsaAI 3430 TACGTG [CT]AC^GT[GA]
BsaHI 150 GACGCC G[GA]^CG[CT]C
BsaI 274 GAGACC GGTCTC(1/5)
BseRI 213 CTCCTC GAGGAG(10/8)
BsgI 237 GTGCAG GTGCAG(16/14)
BsiWI 3428 CGTACG C^GTACG
BspDI 2115 ATCGAT AT^CGAT
BspHI 1685 TCATGA T^CATGA
BspLU11I 34 ACATGT A^CATGT
BspMI 256 ACCTGC ACCTGC(4/8)
BsrBI 332 GAGCGG CCGCTC(-3/-3)
BssSI 783 CTCGTG CACGAG(-5/-1)
BstAPI 280 GCATCCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 203 GGATCC [GA]^GATC[CT]
CelII 1472 GCTTAGC GC^T[ACGT]AGC
ClaI 2115 ATCGAT AT^CGAT
DraII 319 GGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 2515 GAAGAG CTCTTC(1/4)
Eco47III 1825 AGCGCT AGC^GCT
EcoO109I 319 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 3623 ATGCAT ATGCA^T
FauI 317 GCGGG CCCGC(4/6)
FbaI 1446 TGATCA T^GATCA
HaeII 1825 AGCGCT [GA]GCGC^[CT]
HapII 1923 CCGG C^CGG
HgaI 150 GACGC GACGC(5/10)
Hin1I 150 GACGCC G[GA]^CG[CT]C
HindIII 3283 AAGCTT A^AGCTT
HpaII 1923 CCGG C^CGG
KpnI 481 GGTACC GGTAC^C
Ksp632I 2515 GAAGAG CTCTTC(1/4)
MflI 203 GGATCC [GA]^GATC[CT]
MspA1I 2812 CAGCTG C[AC]G^C[GT]G
MspI 1923 CCGG C^CGG
NciI 1922 CCCGG CC^[GC]GG
NsiI 3623 ATGCAT ATGCA^T
PacI 3268 TTAATTAA TTAAT^TAA
PflFI 651 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 319 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 2812 CAGCTG CAG^CTG
RcaI 1685 TCATGA T^CATGA
SexAI 1947 ACCAGGT A^CC[AT]GGT
SmlI 596 CTCAAG C^T[CT][GA]AG
Tth111I 651 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 203 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AocI 488 CCTTAGG CC^T[ACGT]AGG
2669 CCTAAGG
AseI 2031 ATTAAT AT^TAAT
2994 ATTAAT
AsnI 2031 ATTAAT AT^TAAT
2994 ATTAAT
Asp700I 1019 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2264 GAAATCTTTC
AspHI 2622 GTGCTC G[AT]GC[AT]^C
2791 GAGCAC
AvaII 254 GGACC G^G[AT]CC
320 GGTCC
BanI 481 GGTACC G^G[CT][GA]CC
2566 GGCACC
BanII 90 GAGCCC G[GA]GC[CT]^C
408 GAGCCC
BbsI 705 GAAGAC GAAGAC(2/6)
3463 GAAGAC
BpuAI 705 GAAGAC GAAGAC(2/6)
3463 GAAGAC
BsiHKAI 2622 GTGCTC G[AT]GC[AT]^C
2791 GAGCAC
BsmFI 1349 GGGAC GGGAC(10/14)
2055 GTCCC
Bsp1407I 37 TGTACA T^GTACA
1537 TGTACA
BsrGI 37 TGTACA T^GTACA
1537 TGTACA
Bsu36I 488 CCTTAGG CC^T[ACGT]AGG
2669 CCTAAGG
CfoI 1096 GCGC GCG^C
1826 GCGC
Cfr13I 254 GGACC G^G[ACGT]CC
320 GGTCC
DsaI 250 CCATGG C^C[GA][CT]GG
262 CCATGG
Eco81I 488 CCTTAGG CC^T[ACGT]AGG
2669 CCTAAGG
EcoNI 95 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2289 CCTTGCGGAGG
EcoRI 1144 GAATTC G^AATTC
1268 GAATTC
HhaI 1096 GCGC GCG^C
1826 GCGC
HinP1I 1096 GCGC G^CGC
1826 GCGC
HincII 585 GTTAAC GT[CT]^[GA]AC
1609 GTTAAC
HindII 585 GTTAAC GT[CT]^[GA]AC
1609 GTTAAC
HpaI 585 GTTAAC GTT^AAC
1609 GTTAAC
MslI 245 CATTGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1593 CACATATATG
MwoI 280 GCATCCACTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
2320 GCCATTTAAGC
NcoI 250 CCATGG C^CATGG
262 CCATGG
NdeI 3626 CATATG CA^TATG
3753 CATATG
NspI 34 ACATGT [GA]CATG^[CT]
1100 ACATGC
PflMI 284 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2764 CCAAGCAGTGG
PshBI 2031 ATTAAT AT^TAAT
2994 ATTAAT
Sau96I 254 GGACC G^G[ACGT]CC
320 GGTCC
ScaI 84 AGTACT AGT^ACT
2204 AGTACT
SfcI 952 CTACAG C^T[GA][CT]AG
1846 CTGTAG
SspBI 37 TGTACA T^GTACA
1537 TGTACA
TaqI 1148 TCGA T^CGA
2116 TCGA
TthHB8I 1148 TCGA T^CGA
2116 TCGA
Van91I 284 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2764 CCAAGCAGTGG
XmnI 1019 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2264 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI BbvI BfaI BmyI
BsaJI BsiYI BslI BsmAI BsmI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI Cac8I DdeI DpnII DraI
DraIII EcoRII EcoT14I Fnu4HI FokI
HinfI HphI ItaI MaeI MaeII
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI ScrFI SfaNI SspI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI