##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AscI Asp718I AspI
AviII AvrII BbeI BcgI BlnI
BsaHI BsaI BseAI BsiWI BsmBI
BspDI BspEI BsrBI BsrDI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DrdI
EcoO65I EcoRI EcoT22I FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MroI MunI NarI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AhdI 1965 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 867 CCTGAGG CC^T[ACGT]AGG
Aor51HI 820 AGCGCT AGC^GCT
Asp700I 395 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1965 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 51 CCCGGG C^[CT]CG[GA]G
BalI 1339 TGGCCA TGG^CCA
BamHI 1387 GGATCC G^GATCC
BciVI 3116 GTATCC GTATCC(6/5)
BclI 2715 TGATCA T^GATCA
BglI 2461 GCCTTCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1601 AGATCT A^GATCT
BlpI 816 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 816 GCTGAGC GC^T[ACGT]AGC
BsaBI 2837 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 424 GCATTC GAATGC(1/-1)
BsoBI 51 CCCGGG C^[CT]CG[GA]G
BspHI 2229 TCATGA T^CATGA
BspLU11I 1333 ACATGT A^CATGT
Bsu36I 867 CCTGAGG CC^T[ACGT]AGG
CelII 816 GCTGAGC GC^T[ACGT]AGC
EagI 42 CGGCCG C^GGCCG
Eam1105I 1965 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 42 CGGCCG C^GGCCG
Eco47III 820 AGCGCT AGC^GCT
Eco52I 42 CGGCCG C^GGCCG
Eco81I 867 CCTGAGG CC^T[ACGT]AGG
EcoRV 3598 GATATC GAT^ATC
FbaI 2715 TGATCA T^GATCA
HindIII 2738 AAGCTT A^AGCTT
MamI 2837 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1339 TGGCCA TGG^CCA
MscI 1339 TGGCCA TGG^CCA
PflMI 2117 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 2229 TCATGA T^CATGA
SapI 228 GAAGAGC GCTCTTC(1/4)
SexAI 1582 ACCTGGT A^CC[AT]GGT
SmaI 51 CCCGGG CCC^GGG
SphI 2457 GCATGC GCATG^C
TaqI 667 TCGA T^CGA
TthHB8I 667 TCGA T^CGA
Van91I 2117 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1692 TCTAGA T^CTAGA
XcmI 2328 CCACCTGTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
XmnI 395 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflII 617 CTTAAG C^TTAAG
2622 CTTAAG
AlwNI 480 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1578 CAGCACCTG
AseI 3528 ATTAAT AT^TAAT
3589 ATTAAT
AsnI 3528 ATTAAT AT^TAAT
3589 ATTAAT
BanI 1444 GGTGCC G^G[CT][GA]CC
2886 GGCACC
BbrPI 2410 CACGTG CAC^GTG
2546 CACGTG
BfrI 617 CTTAAG C^TTAAG
2622 CTTAAG
BsaWI 77 TCCGGT [AT]^CCGG[AT]
1249 ACCGGA
BsiEI 42 CGGCCG CG[GA][CT]^CG
668 CGACCG
Bsp1407I 487 TGTACA T^GTACA
3517 TGTACA
BsrGI 487 TGTACA T^GTACA
3517 TGTACA
BstXI 1537 CCACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1924 CCATGCACTTGG
EarI 228 GAAGAG CTCTTC(1/4)
1984 GAAGAG
EcoNI 605 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
863 CCTTCCTGAGG
HaeII 820 AGCGCT [GA]GCGC^[CT]
1024 GGCGCT
Ksp632I 228 GAAGAG CTCTTC(1/4)
1984 GAAGAG
NaeI 133 GCCGGC GCC^GGC
148 GCCGGC
NcoI 385 CCATGG C^CATGG
2930 CCATGG
NdeI 2471 CATATG CA^TATG
3521 CATATG
NgoMIV 133 GCCGGC G^CCGGC
148 GCCGGC
PmaCI 2410 CACGTG CAC^GTG
2546 CACGTG
PmlI 2410 CACGTG CAC^GTG
2546 CACGTG
PshAI 1505 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1563 GACCCTTGTC
PshBI 3528 ATTAAT AT^TAAT
3589 ATTAAT
PstI 453 CTGCAG CTGCA^G
3336 CTGCAG
SspBI 487 TGTACA T^GTACA
3517 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI ApaI ApaLI
ApoI AspHI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII