##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspEI AvaI AviII BamHI BcgI
BfrI BlpI Bpu1102I BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspEI BspLU11I BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I CelII CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
FseI FspI HpaI KpnI KspI
MfeI MluI MroI MunI NaeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 141 CGCG CG^CG
AcyI 129 GGCGCC G[GA]^CG[CT]C
AflIII 2485 ACACGT A^C[GA][CT]GT
AocI 1634 CCTCAGG CC^T[ACGT]AGG
ApaI 3563 GGGCCC GGGCC^C
AspI 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 478 GGACC G^G[AT]CC
AvrII 786 CCTAGG C^CTAGG
BalI 1384 TGGCCA TGG^CCA
BbeI 129 GGCGCC GGCGC^C
BbrPI 2486 CACGTG CAC^GTG
BciVI 2197 GTATCC GTATCC(6/5)
BcnI 136 CCGGG CC^[GC]GG
BglI 192 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 423 AGATCT A^GATCT
BlnI 786 CCTAGG C^CTAGG
BsaBI 835 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 129 GGCGCC G[GA]^CG[CT]C
BsaI 2209 GGTCTC GGTCTC(1/5)
BspDI 2008 ATCGAT AT^CGAT
BspHI 2705 TCATGA T^CATGA
BsrDI 2835 GCAATG GCAATG(2/0)
BsrFI 1767 ACCGGC [GA]^CCGG[CT]
BstAPI 492 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 141 CGCG CG^CG
Bsu36I 1634 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1767 ACCGGC [GA]^CCGG[CT]
ClaI 2008 ATCGAT AT^CGAT
DraIII 449 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 380 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1634 CCTCAGG CC^T[ACGT]AGG
EcoNI 662 CCTAGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1598 GACGC GACGC(5/10)
Hin1I 129 GGCGCC G[GA]^CG[CT]C
HincII 3209 GTTGAC GT[CT]^[GA]AC
HindII 3209 GTTGAC GT[CT]^[GA]AC
HindIII 1141 AAGCTT A^AGCTT
KasI 129 GGCGCC G^GCGCC
MamI 835 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1384 TGGCCA TGG^CCA
MscI 1384 TGGCCA TGG^CCA
MspA1I 3842 CAGCTG C[AC]G^C[GT]G
MvnI 141 CGCG CG^CG
NarI 129 GGCGCC GG^CGCC
NciI 136 CCGGG CC^[GC]GG
NdeI 3607 CATATG CA^TATG
PflFI 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2486 CACGTG CAC^GTG
PmlI 2486 CACGTG CAC^GTG
PpuMI 477 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 3682 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 3842 CAGCTG CAG^CTG
RcaI 2705 TCATGA T^CATGA
SapI 233 GCTCTTC GCTCTTC(1/4)
SfoI 129 GGCGCC GGC^GCC
Tth111I 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 1377 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1823 CAGCGACTG
BanI 129 GGCGCC G^G[CT][GA]CC
3473 GGCACC
BbvCI 974 GCTGAGG CCTCAGC(-5/-2)
1207 GCTGAGG
BclI 321 TGATCA T^GATCA
3582 TGATCA
BsaAI 2486 CACGTG [CT]AC^GT[GA]
3064 CACGTA
BseRI 285 CTCCTC GAGGAG(10/8)
1311 GAGGAG
DraI 1125 TTTAAA TTT^AAA
3426 TTTAAA
DraII 477 AGGACCT [GA]G^G[ACGT]CC[CT]
3563 GGGCCCC
EaeI 143 CGGCCA [CT]^GGCC[GA]
1384 TGGCCA
EcoO109I 477 AGGACCT [GA]G^G[ACGT]CC[CT]
3563 GGGCCCC
EcoRI 1109 GAATTC G^AATTC
2803 GAATTC
EcoT22I 1078 ATGCAT ATGCA^T
3090 ATGCAT
FauI 201 GCGGG CCCGC(4/6)
2248 GCGGG
FbaI 321 TGATCA T^GATCA
3582 TGATCA
NcoI 2117 CCATGG C^CATGG
3732 CCATGG
NsiI 1078 ATGCAT ATGCA^T
3090 ATGCAT
PflMI 448 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCAGAACCTGG
PstI 3447 CTGCAG CTGCA^G
3709 CTGCAG
SmlI 1934 CTCAAG C^T[CT][GA]AG
3692 CTCAAG
SspI 694 AATATT AAT^ATT
2028 AATATT
StuI 2506 AGGCCT AGG^CCT
3796 AGGCCT
Van91I 448 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1376 CCAGAACCTGG
XcmI 451 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3511 CCAGCACTCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BanII BbsI
BbvI BfaI BmyI BpmI BpuAI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI