################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp718I AspEI AvaI AviII BamHI BcgI BfrI BlpI Bpu1102I BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspEI BspLU11I BsrBI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccII 141 CGCG CG^CG AcyI 129 GGCGCC G[GA]^CG[CT]C AflIII 2485 ACACGT A^C[GA][CT]GT AocI 1634 CCTCAGG CC^T[ACGT]AGG ApaI 3563 GGGCCC GGGCC^C AspI 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 478 GGACC G^G[AT]CC AvrII 786 CCTAGG C^CTAGG BalI 1384 TGGCCA TGG^CCA BbeI 129 GGCGCC GGCGC^C BbrPI 2486 CACGTG CAC^GTG BciVI 2197 GTATCC GTATCC(6/5) BcnI 136 CCGGG CC^[GC]GG BglI 192 GCCCAGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 423 AGATCT A^GATCT BlnI 786 CCTAGG C^CTAGG BsaBI 835 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 129 GGCGCC G[GA]^CG[CT]C BsaI 2209 GGTCTC GGTCTC(1/5) BspDI 2008 ATCGAT AT^CGAT BspHI 2705 TCATGA T^CATGA BsrDI 2835 GCAATG GCAATG(2/0) BsrFI 1767 ACCGGC [GA]^CCGG[CT] BstAPI 492 GCAGCCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 141 CGCG CG^CG Bsu36I 1634 CCTCAGG CC^T[ACGT]AGG Cfr10I 1767 ACCGGC [GA]^CCGG[CT] ClaI 2008 ATCGAT AT^CGAT DraIII 449 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 380 GACCACACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1634 CCTCAGG CC^T[ACGT]AGG EcoNI 662 CCTAGATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HgaI 1598 GACGC GACGC(5/10) Hin1I 129 GGCGCC G[GA]^CG[CT]C HincII 3209 GTTGAC GT[CT]^[GA]AC HindII 3209 GTTGAC GT[CT]^[GA]AC HindIII 1141 AAGCTT A^AGCTT KasI 129 GGCGCC G^GCGCC MamI 835 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1384 TGGCCA TGG^CCA MscI 1384 TGGCCA TGG^CCA MspA1I 3842 CAGCTG C[AC]G^C[GT]G MvnI 141 CGCG CG^CG NarI 129 GGCGCC GG^CGCC NciI 136 CCGGG CC^[GC]GG NdeI 3607 CATATG CA^TATG PflFI 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2486 CACGTG CAC^GTG PmlI 2486 CACGTG CAC^GTG PpuMI 477 AGGACCT [GA]G^G[AT]CC[CT] PshAI 3682 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 3842 CAGCTG CAG^CTG RcaI 2705 TCATGA T^CATGA SapI 233 GCTCTTC GCTCTTC(1/4) SfoI 129 GGCGCC GGC^GCC Tth111I 672 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwNI 1377 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG 1823 CAGCGACTG BanI 129 GGCGCC G^G[CT][GA]CC 3473 GGCACC BbvCI 974 GCTGAGG CCTCAGC(-5/-2) 1207 GCTGAGG BclI 321 TGATCA T^GATCA 3582 TGATCA BsaAI 2486 CACGTG [CT]AC^GT[GA] 3064 CACGTA BseRI 285 CTCCTC GAGGAG(10/8) 1311 GAGGAG DraI 1125 TTTAAA TTT^AAA 3426 TTTAAA DraII 477 AGGACCT [GA]G^G[ACGT]CC[CT] 3563 GGGCCCC EaeI 143 CGGCCA [CT]^GGCC[GA] 1384 TGGCCA EcoO109I 477 AGGACCT [GA]G^G[ACGT]CC[CT] 3563 GGGCCCC EcoRI 1109 GAATTC G^AATTC 2803 GAATTC EcoT22I 1078 ATGCAT ATGCA^T 3090 ATGCAT FauI 201 GCGGG CCCGC(4/6) 2248 GCGGG FbaI 321 TGATCA T^GATCA 3582 TGATCA NcoI 2117 CCATGG C^CATGG 3732 CCATGG NsiI 1078 ATGCAT ATGCA^T 3090 ATGCAT PflMI 448 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1376 CCAGAACCTGG PstI 3447 CTGCAG CTGCA^G 3709 CTGCAG SmlI 1934 CTCAAG C^T[CT][GA]AG 3692 CTCAAG SspI 694 AATATT AAT^ATT 2028 AATATT StuI 2506 AGGCCT AGG^CCT 3796 AGGCCT Van91I 448 CCACCATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1376 CCAGAACCTGG XcmI 451 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3511 CCAGCACTCACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 88 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DsaI EarI EcoRII EcoRV EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV NspI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI