##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AcyI AhdI
Alw44I Aor51HI ApaLI AscI Asp718I
AspEI BamHI BbeI BbvCI BglII
BsaHI BspDI BsrBI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRV EcoT22I FseI Hin1I HincII
HindII HpaI KasI KpnI MluI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
Psp1406I PstI PvuI RsrII SacI
SalI SbfI ScaI SfiI SfoI
SfuI SgrAI Sse8387I SspI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccI 2860 GTAGAC GT^[AC][GT]AC
AccIII 1824 TCCGGA T^CCGGA
AflII 1489 CTTAAG C^TTAAG
AgeI 2615 ACCGGT A^CCGGT
AocI 1693 CCTGAGG CC^T[ACGT]AGG
ApaI 80 GGGCCC GGGCC^C
AseI 3163 ATTAAT AT^TAAT
AsnI 3163 ATTAAT AT^TAAT
Asp700I 1203 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2779 TGCGCA TGC^GCA
AvrII 2468 CCTAGG C^CTAGG
BbrPI 1195 CACGTG CAC^GTG
BciVI 2387 GTATCC GTATCC(6/5)
BclI 1464 TGATCA T^GATCA
BfrI 1489 CTTAAG C^TTAAG
BglI 335 GCCTGGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2468 CCTAGG C^CTAGG
BseAI 1824 TCCGGA T^CCGGA
BsiEI 1341 CGACCG CG[GA][CT]^CG
BsiWI 3352 CGTACG C^GTACG
BsmBI 616 GAGACG CGTCTC(1/5)
BsmI 440 GAATGC GAATGC(1/-1)
BspEI 1824 TCCGGA T^CCGGA
BspMI 2540 GCAGGT ACCTGC(4/8)
BssHII 3371 GCGCGC G^CGCGC
BstXI 1379 CCAGCTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1693 CCTGAGG CC^T[ACGT]AGG
Eco81I 1693 CCTGAGG CC^T[ACGT]AGG
EcoNI 1208 CCTTCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 991 GAATTC G^AATTC
FbaI 1464 TGATCA T^GATCA
FspI 2779 TGCGCA TGC^GCA
HindIII 2497 AAGCTT A^AGCTT
KspI 1178 CCGCGG CCGC^GG
MfeI 3550 CAATTG C^AATTG
MroI 1824 TCCGGA T^CCGGA
MunI 3550 CAATTG C^AATTG
NaeI 1405 GCCGGC GCC^GGC
NcoI 1282 CCATGG C^CATGG
NdeI 2334 CATATG CA^TATG
NgoMIV 1405 GCCGGC G^CCGGC
PinAI 2615 ACCGGT A^CCGGT
PmaCI 1195 CACGTG CAC^GTG
PmlI 1195 CACGTG CAC^GTG
PshBI 3163 ATTAAT AT^TAAT
SacII 1178 CCGCGG CCGC^GG
SfcI 3155 CTGTAG C^T[GA][CT]AG
SmaI 77 CCCGGG CCC^GGG
SnaBI 2857 TACGTA TAC^GTA
SpeI 2926 ACTAGT A^CTAGT
StuI 776 AGGCCT AGG^CCT
XcmI 1856 CCACATGAGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 77 CCCGGG C^CCGGG
XmaI 77 CCCGGG C^CCGGG
XmnI 1203 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 1179 CGCG CG^CG
3372 CGCG
BalI 212 TGGCCA TGG^CCA
663 TGGCCA
BanI 2618 GGTGCC G^G[CT][GA]CC
3412 GGCACC
BcgI 630 CGAGATGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1526 CGAGCGTCCTGC
BlpI 1734 GCTGAGC GC^T[ACGT]AGC
2585 GCTAAGC
Bpu1102I 1734 GCTGAGC GC^T[ACGT]AGC
2585 GCTAAGC
BsaAI 1195 CACGTG [CT]AC^GT[GA]
2857 TACGTA
BsaBI 1987 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2443 GATCGACATC
Bsp1407I 347 TGTACA T^GTACA
3461 TGTACA
BsrGI 347 TGTACA T^GTACA
3461 TGTACA
BssSI 922 CTCGTG CACGAG(-5/-1)
3342 CTCGTG
BstUI 1179 CGCG CG^CG
3372 CGCG
BstYI 482 GGATCT [GA]^GATC[CT]
886 GGATCT
CelII 1734 GCTGAGC GC^T[ACGT]AGC
2585 GCTAAGC
DsaI 1178 CCGCGG C^C[GA][CT]GG
1282 CCATGG
HgaI 2757 GACGC GACGC(5/10)
1529 GCGTC
MamI 1987 GATTGTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2443 GATCGACATC
MflI 482 GGATCT [GA]^GATC[CT]
886 GGATCT
MluNI 212 TGGCCA TGG^CCA
663 TGGCCA
MscI 212 TGGCCA TGG^CCA
663 TGGCCA
MvnI 1179 CGCG CG^CG
3372 CGCG
SapI 1248 GCTCTTC GCTCTTC(1/4)
1637 GCTCTTC
SexAI 2725 ACCAGGT A^CC[AT]GGT
3080 ACCAGGT
SspBI 347 TGTACA T^GTACA
3461 TGTACA
XhoII 482 GGATCT [GA]^GATC[CT]
886 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AspI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspHI BspLU11I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PpuMI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI SphI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I