##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccII AccIII AclI AgeI
AhdI Aor51HI AscI AseI AsnI
Asp718I AspEI AspI BamHI BbeI
BcgI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV FseI HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AvaI 2495 CCCGAG C^[CT]CG[GA]G
AviII 2211 TGCGCA TGC^GCA
AvrII 393 CCTAGG C^CTAGG
BalI 3971 TGGCCA TGG^CCA
BciVI 567 GTATCC GTATCC(6/5)
BlnI 393 CCTAGG C^CTAGG
BlpI 4490 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4490 GCTCAGC GC^T[ACGT]AGC
BsmBI 1902 CGTCTC CGTCTC(1/5)
BsoBI 2495 CCCGAG C^[CT]CG[GA]G
BspHI 3930 TCATGA T^CATGA
BspMI 3831 GCAGGT ACCTGC(4/8)
BsrBI 2588 GAGCGG CCGCTC(-3/-3)
BstEII 3787 GGTGACC G^GT[ACGT]ACC
BstPI 3787 GGTGACC G^GT[ACGT]ACC
CelII 4490 GCTCAGC GC^T[ACGT]AGC
DraIII 4180 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 3787 GGTGACC G^GT[ACGT]ACC
EcoT22I 3187 ATGCAT ATGCA^T
FspI 2211 TGCGCA TGC^GCA
HaeII 4006 GGCGCT [GA]GCGC^[CT]
MluNI 3971 TGGCCA TGG^CCA
MscI 3971 TGGCCA TGG^CCA
NdeI 848 CATATG CA^TATG
NsiI 3187 ATGCAT ATGCA^T
PshAI 1283 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3930 TCATGA T^CATGA
SacI 2114 GAGCTC GAGCT^C
StuI 1661 AGGCCT AGG^CCT
TaqI 2511 TCGA T^CGA
TthHB8I 2511 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AatII 2773 GACGTC GACGT^C
4596 GACGTC
AccI 2411 GTCTAC GT^[AC][GT]AC
3141 GTCTAC
AcyI 2773 GACGTC G[GA]^CG[CT]C
4596 GACGTC
AflII 334 CTTAAG C^TTAAG
4382 CTTAAG
AocI 3577 CCTAAGG CC^T[ACGT]AGG
4467 CCTTAGG
ApaI 2076 GGGCCC GGGCC^C
3334 GGGCCC
Asp700I 1438 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2787 GAAGGCATTC
BanI 4585 GGTGCC G^G[CT][GA]CC
4721 GGCACC
BbrPI 1348 CACGTG CAC^GTG
4531 CACGTG
BbsI 1492 GAAGAC GAAGAC(2/6)
170 GTCTTC
BclI 2169 TGATCA T^GATCA
2849 TGATCA
BcnI 571 CCCGG CC^[GC]GG
3462 CCGGG
BfrI 334 CTTAAG C^TTAAG
4382 CTTAAG
BglI 1388 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4020 GCCTGAAGGGC
BpuAI 1492 GAAGAC GAAGAC(2/6)
170 GTCTTC
BsaHI 2773 GACGTC G[GA]^CG[CT]C
4596 GACGTC
Bsp1407I 1035 TGTACA T^GTACA
2517 TGTACA
BspLU11I 1038 ACATGT A^CATGT
3603 ACATGT
BsrDI 3975 CATTGC GCAATG(2/0)
1952 GCAATG
BsrGI 1035 TGTACA T^GTACA
2517 TGTACA
BstXI 2539 CCACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4642 CCATTGTGCTGG
Bsu36I 3577 CCTAAGG CC^T[ACGT]AGG
4467 CCTTAGG
Eco81I 3577 CCTAAGG CC^T[ACGT]AGG
4467 CCTTAGG
FbaI 2169 TGATCA T^GATCA
2849 TGATCA
HgaI 2377 GACGC GACGC(5/10)
1998 GCGTC
Hin1I 2773 GACGTC G[GA]^CG[CT]C
4596 GACGTC
NciI 571 CCCGG CC^[GC]GG
3462 CCGGG
PmaCI 1348 CACGTG CAC^GTG
4531 CACGTG
PmlI 1348 CACGTG CAC^GTG
4531 CACGTG
SphI 344 GCATGC GCATG^C
517 GCATGC
SspBI 1035 TGTACA T^GTACA
2517 TGTACA
XmnI 1438 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2787 GAAGGCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BanII BbvCI BbvI
BfaI BglII BmyI BpmI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstAPI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII