################################## # NO CUT ENZYMES ( 90 enzymes) # ################################## Acc65I AccII AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI BamHI BbeI BcgI BsaBI BsaWI BseAI BsiEI BsiWI BspDI BspEI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FseI HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AvaI 2495 CCCGAG C^[CT]CG[GA]G AviII 2211 TGCGCA TGC^GCA AvrII 393 CCTAGG C^CTAGG BalI 3971 TGGCCA TGG^CCA BciVI 567 GTATCC GTATCC(6/5) BlnI 393 CCTAGG C^CTAGG BlpI 4490 GCTCAGC GC^T[ACGT]AGC Bpu1102I 4490 GCTCAGC GC^T[ACGT]AGC BsmBI 1902 CGTCTC CGTCTC(1/5) BsoBI 2495 CCCGAG C^[CT]CG[GA]G BspHI 3930 TCATGA T^CATGA BspMI 3831 GCAGGT ACCTGC(4/8) BsrBI 2588 GAGCGG CCGCTC(-3/-3) BstEII 3787 GGTGACC G^GT[ACGT]ACC BstPI 3787 GGTGACC G^GT[ACGT]ACC CelII 4490 GCTCAGC GC^T[ACGT]AGC DraIII 4180 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 3787 GGTGACC G^GT[ACGT]ACC EcoT22I 3187 ATGCAT ATGCA^T FspI 2211 TGCGCA TGC^GCA HaeII 4006 GGCGCT [GA]GCGC^[CT] MluNI 3971 TGGCCA TGG^CCA MscI 3971 TGGCCA TGG^CCA NdeI 848 CATATG CA^TATG NsiI 3187 ATGCAT ATGCA^T PshAI 1283 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3930 TCATGA T^CATGA SacI 2114 GAGCTC GAGCT^C StuI 1661 AGGCCT AGG^CCT TaqI 2511 TCGA T^CGA TthHB8I 2511 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AatII 2773 GACGTC GACGT^C 4596 GACGTC AccI 2411 GTCTAC GT^[AC][GT]AC 3141 GTCTAC AcyI 2773 GACGTC G[GA]^CG[CT]C 4596 GACGTC AflII 334 CTTAAG C^TTAAG 4382 CTTAAG AocI 3577 CCTAAGG CC^T[ACGT]AGG 4467 CCTTAGG ApaI 2076 GGGCCC GGGCC^C 3334 GGGCCC Asp700I 1438 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2787 GAAGGCATTC BanI 4585 GGTGCC G^G[CT][GA]CC 4721 GGCACC BbrPI 1348 CACGTG CAC^GTG 4531 CACGTG BbsI 1492 GAAGAC GAAGAC(2/6) 170 GTCTTC BclI 2169 TGATCA T^GATCA 2849 TGATCA BcnI 571 CCCGG CC^[GC]GG 3462 CCGGG BfrI 334 CTTAAG C^TTAAG 4382 CTTAAG BglI 1388 GCCACTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4020 GCCTGAAGGGC BpuAI 1492 GAAGAC GAAGAC(2/6) 170 GTCTTC BsaHI 2773 GACGTC G[GA]^CG[CT]C 4596 GACGTC Bsp1407I 1035 TGTACA T^GTACA 2517 TGTACA BspLU11I 1038 ACATGT A^CATGT 3603 ACATGT BsrDI 3975 CATTGC GCAATG(2/0) 1952 GCAATG BsrGI 1035 TGTACA T^GTACA 2517 TGTACA BstXI 2539 CCACTTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4642 CCATTGTGCTGG Bsu36I 3577 CCTAAGG CC^T[ACGT]AGG 4467 CCTTAGG Eco81I 3577 CCTAAGG CC^T[ACGT]AGG 4467 CCTTAGG FbaI 2169 TGATCA T^GATCA 2849 TGATCA HgaI 2377 GACGC GACGC(5/10) 1998 GCGTC Hin1I 2773 GACGTC G[GA]^CG[CT]C 4596 GACGTC NciI 571 CCCGG CC^[GC]GG 3462 CCGGG PmaCI 1348 CACGTG CAC^GTG 4531 CACGTG PmlI 1348 CACGTG CAC^GTG 4531 CACGTG SphI 344 GCATGC GCATG^C 517 GCATGC SspBI 1035 TGTACA T^GTACA 2517 TGTACA XmnI 1438 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2787 GAAGGCATTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BanII BbvCI BbvI BfaI BglII BmyI BpmI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII