##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccIII AclI AgeI Aor51HI AscI
AseI AsnI AspI BbrPI BciVI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssSI ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI HpaI
KspI MfeI MluI MroI MunI
NotI NruI NsiI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI ScaI SfiI
SgrAI SnaBI SphI Sse8387I SspBI
SspI SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 679 GACGTC GACGT^C
Acc65I 5259 GGTACC G^GTACC
AccI 1181 GTATAC GT^[AC][GT]AC
AcsI 5064 AAATTT [GA]^AATT[CT]
Alw44I 1734 GTGCAC G^TGCAC
ApaLI 1734 GTGCAC G^TGCAC
ApoI 5064 AAATTT [GA]^AATT[CT]
Asp700I 464 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 5259 GGTACC G^GTACC
AviII 3807 TGCGCA TGC^GCA
BbeI 3018 GGCGCC GGCGC^C
BclI 2714 TGATCA T^GATCA
BglII 2436 AGATCT A^GATCT
BsaBI 361 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4872 ACCGGA [AT]^CCGG[AT]
BspHI 3693 TCATGA T^CATGA
BsrFI 1313 GCCGGC [GA]^CCGG[CT]
BssHII 1962 GCGCGC G^CGCGC
Bst1107I 1181 GTATAC GTA^TAC
BstAPI 1075 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1181 GTATAC GTA^TAC
Cfr10I 1313 GCCGGC [GA]^CCGG[CT]
DraI 85 TTTAAA TTT^AAA
DraIII 2748 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2537 GACAGCTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2714 TGATCA T^GATCA
FspI 3807 TGCGCA TGC^GCA
HgaI 3972 GACGC GACGC(5/10)
HincII 1950 GTTGAC GT[CT]^[GA]AC
HindII 1950 GTTGAC GT[CT]^[GA]AC
KasI 3018 GGCGCC G^GCGCC
KpnI 5259 GGTACC GGTAC^C
MamI 361 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1313 GCCGGC GCC^GGC
NarI 3018 GGCGCC GG^CGCC
NgoMIV 1313 GCCGGC G^CCGGC
NheI 6 GCTAGC G^CTAGC
PacI 5357 TTAATTAA TTAAT^TAA
RcaI 3693 TCATGA T^CATGA
SacI 3770 GAGCTC GAGCT^C
SfoI 3018 GGCGCC GGC^GCC
SmaI 2576 CCCGGG CCC^GGG
SpeI 934 ACTAGT A^CTAGT
XbaI 3641 TCTAGA T^CTAGA
XcmI 1796 CCAGCCTCTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2576 CCCGGG C^CCGGG
XmaI 2576 CCCGGG C^CCGGG
XmnI 464 GAACTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 679 GACGTC G[GA]^CG[CT]C
3018 GGCGCC
AflII 4660 CTTAAG C^TTAAG
4722 CTTAAG
AflIII 2496 ACACGT A^C[GA][CT]GT
4596 ACACGT
AvrII 3817 CCTAGG C^CTAGG
5286 CCTAGG
BamHI 1335 GGATCC G^GATCC
4018 GGATCC
BfrI 4660 CTTAAG C^TTAAG
4722 CTTAAG
BlnI 3817 CCTAGG C^CTAGG
5286 CCTAGG
BsaAI 3894 TACGTG [CT]AC^GT[GA]
4597 CACGTA
BsaHI 679 GACGTC G[GA]^CG[CT]C
3018 GGCGCC
BsmBI 1923 CGTCTC CGTCTC(1/5)
2502 CGTCTC
BsrBI 698 CCGCTC CCGCTC(-3/-3)
5263 CCGCTC
BstBI 1859 TTCGAA TT^CGAA
4168 TTCGAA
BstEII 1000 GGTAACC G^GT[ACGT]ACC
4361 GGTTACC
BstPI 1000 GGTAACC G^GT[ACGT]ACC
4361 GGTTACC
EcoO65I 1000 GGTAACC G^GT[ACGT]ACC
4361 GGTTACC
Hin1I 679 GACGTC G[GA]^CG[CT]C
3018 GGCGCC
NspV 1859 TTCGAA TT^CGAA
4168 TTCGAA
SexAI 507 ACCAGGT A^CC[AT]GGT
1703 ACCTGGT
SfuI 1859 TTCGAA TT^CGAA
4168 TTCGAA
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AfaI AhdI AluI
AlwI AlwNI AocI ApaI AspEI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcgI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI Eam1105I
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII