################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AhdI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BcgI BfrI BglI BlnI BseAI BsiWI BsmBI BspEI BssSI Bst1107I BstBI BstEII BstPI BstZ17I DraII Eam1105I EcoO109I EcoO65I EcoRV FseI FspI HpaI KpnI KspI MfeI MluI MroI MunI NdeI NotI NruI NspV PacI PflFI PmeI PpuMI PshAI PshBI Psp1406I SacII SalI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 799 GTAGAC GT^[AC][GT]AC AcyI 129 GGCGCC G[GA]^CG[CT]C AgeI 3065 ACCGGT A^CCGGT AocI 2644 CCTCAGG CC^T[ACGT]AGG Aor51HI 2780 AGCGCT AGC^GCT ApaI 12 GGGCCC GGGCC^C Asp700I 744 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BamHI 3679 GGATCC G^GATCC BbeI 129 GGCGCC GGCGC^C BglII 3509 AGATCT A^GATCT BsaBI 1615 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 129 GGCGCC G[GA]^CG[CT]C BsaWI 3065 ACCGGT [AT]^CCGG[AT] BspDI 1513 ATCGAT AT^CGAT BspHI 3335 TCATGA T^CATGA BspLU11I 1675 ACATGT A^CATGT BspMI 578 ACCTGC ACCTGC(4/8) BsrBI 2740 GAGCGG CCGCTC(-3/-3) BssHII 165 GCGCGC G^CGCGC Bsu36I 2644 CCTCAGG CC^T[ACGT]AGG ClaI 1513 ATCGAT AT^CGAT CpoI 76 CGGACCG CG^G[AT]CCG DraIII 1756 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 613 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 72 CGGCCG C^GGCCG EclXI 72 CGGCCG C^GGCCG Eco47III 2780 AGCGCT AGC^GCT Eco52I 72 CGGCCG C^GGCCG Eco81I 2644 CCTCAGG CC^T[ACGT]AGG EcoT22I 2161 ATGCAT ATGCA^T Hin1I 129 GGCGCC G[GA]^CG[CT]C HincII 4175 GTTGAC GT[CT]^[GA]AC HindII 4175 GTTGAC GT[CT]^[GA]AC KasI 129 GGCGCC G^GCGCC MamI 1615 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 129 GGCGCC GG^CGCC NheI 3591 GCTAGC G^CTAGC NsiI 2161 ATGCAT ATGCA^T PaeR7I 2831 CTCGAG C^TCGAG PflMI 3683 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 3065 ACCGGT A^CCGGT PvuI 583 CGATCG CGAT^CG RcaI 3335 TCATGA T^CATGA RsrII 76 CGGACCG CG^G[AT]CCG SacI 3298 GAGCTC GAGCT^C SfoI 129 GGCGCC GGC^GCC SmaI 2553 CCCGGG CCC^GGG SmlI 2831 CTCGAG C^T[CT][GA]AG SphI 1460 GCATGC GCATG^C SspI 223 AATATT AAT^ATT StuI 3099 AGGCCT AGG^CCT Van91I 3683 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 658 CCACCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 2831 CTCGAG C^TCGAG XmaCI 2553 CCCGGG C^CCGGG XmaI 2553 CCCGGG C^CCGGG XmnI 744 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 1675 ACATGT A^C[GA][CT]GT 2123 ACGTGT Alw44I 595 GTGCAC G^TGCAC 1815 GTGCAC ApaLI 595 GTGCAC G^TGCAC 1815 GTGCAC BbrPI 547 CACGTG CAC^GTG 607 CACGTG BciVI 1348 GGATAC GTATCC(6/5) 4004 GGATAC BclI 792 TGATCA T^GATCA 3426 TGATCA BlpI 2332 GCTCAGC GC^T[ACGT]AGC 2337 GCTCAGC Bpu1102I 2332 GCTCAGC GC^T[ACGT]AGC 2337 GCTCAGC BsaAI 547 CACGTG [CT]AC^GT[GA] 607 CACGTG BsmI 1253 GAATGC GAATGC(1/-1) 3521 GCATTC Bsp1407I 1061 TGTACA T^GTACA 1617 TGTACA BsrGI 1061 TGTACA T^GTACA 1617 TGTACA BstAPI 597 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1455 GCAAGGCATGC CelII 2332 GCTCAGC GC^T[ACGT]AGC 2337 GCTCAGC DraI 918 TTTAAA TTT^AAA 3731 TTTAAA FbaI 792 TGATCA T^GATCA 3426 TGATCA HaeII 129 GGCGCC [GA]GCGC^[CT] 2780 AGCGCT HgaI 1586 GACGC GACGC(5/10) 1737 GCGTC PmaCI 547 CACGTG CAC^GTG 607 CACGTG PmlI 547 CACGTG CAC^GTG 607 CACGTG PvuII 317 CAGCTG CAG^CTG 3377 CAGCTG SexAI 2463 ACCTGGT A^CC[AT]GGT 2537 ACCAGGT SspBI 1061 TGTACA T^GTACA 1617 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI RsaI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII