##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AhdI AscI AseI AsnI Asp718I
AspEI AspI AviII AvrII BcgI
BfrI BglI BlnI BseAI BsiWI
BsmBI BspEI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I DraII Eam1105I
EcoO109I EcoO65I EcoRV FseI FspI
HpaI KpnI KspI MfeI MluI
MroI MunI NdeI NotI NruI
NspV PacI PflFI PmeI PpuMI
PshAI PshBI Psp1406I SacII SalI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 799 GTAGAC GT^[AC][GT]AC
AcyI 129 GGCGCC G[GA]^CG[CT]C
AgeI 3065 ACCGGT A^CCGGT
AocI 2644 CCTCAGG CC^T[ACGT]AGG
Aor51HI 2780 AGCGCT AGC^GCT
ApaI 12 GGGCCC GGGCC^C
Asp700I 744 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 3679 GGATCC G^GATCC
BbeI 129 GGCGCC GGCGC^C
BglII 3509 AGATCT A^GATCT
BsaBI 1615 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 129 GGCGCC G[GA]^CG[CT]C
BsaWI 3065 ACCGGT [AT]^CCGG[AT]
BspDI 1513 ATCGAT AT^CGAT
BspHI 3335 TCATGA T^CATGA
BspLU11I 1675 ACATGT A^CATGT
BspMI 578 ACCTGC ACCTGC(4/8)
BsrBI 2740 GAGCGG CCGCTC(-3/-3)
BssHII 165 GCGCGC G^CGCGC
Bsu36I 2644 CCTCAGG CC^T[ACGT]AGG
ClaI 1513 ATCGAT AT^CGAT
CpoI 76 CGGACCG CG^G[AT]CCG
DraIII 1756 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 613 GACATGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 72 CGGCCG C^GGCCG
EclXI 72 CGGCCG C^GGCCG
Eco47III 2780 AGCGCT AGC^GCT
Eco52I 72 CGGCCG C^GGCCG
Eco81I 2644 CCTCAGG CC^T[ACGT]AGG
EcoT22I 2161 ATGCAT ATGCA^T
Hin1I 129 GGCGCC G[GA]^CG[CT]C
HincII 4175 GTTGAC GT[CT]^[GA]AC
HindII 4175 GTTGAC GT[CT]^[GA]AC
KasI 129 GGCGCC G^GCGCC
MamI 1615 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 129 GGCGCC GG^CGCC
NheI 3591 GCTAGC G^CTAGC
NsiI 2161 ATGCAT ATGCA^T
PaeR7I 2831 CTCGAG C^TCGAG
PflMI 3683 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 3065 ACCGGT A^CCGGT
PvuI 583 CGATCG CGAT^CG
RcaI 3335 TCATGA T^CATGA
RsrII 76 CGGACCG CG^G[AT]CCG
SacI 3298 GAGCTC GAGCT^C
SfoI 129 GGCGCC GGC^GCC
SmaI 2553 CCCGGG CCC^GGG
SmlI 2831 CTCGAG C^T[CT][GA]AG
SphI 1460 GCATGC GCATG^C
SspI 223 AATATT AAT^ATT
StuI 3099 AGGCCT AGG^CCT
Van91I 3683 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 658 CCACCAGAGGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2831 CTCGAG C^TCGAG
XmaCI 2553 CCCGGG C^CCGGG
XmaI 2553 CCCGGG C^CCGGG
XmnI 744 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AflIII 1675 ACATGT A^C[GA][CT]GT
2123 ACGTGT
Alw44I 595 GTGCAC G^TGCAC
1815 GTGCAC
ApaLI 595 GTGCAC G^TGCAC
1815 GTGCAC
BbrPI 547 CACGTG CAC^GTG
607 CACGTG
BciVI 1348 GGATAC GTATCC(6/5)
4004 GGATAC
BclI 792 TGATCA T^GATCA
3426 TGATCA
BlpI 2332 GCTCAGC GC^T[ACGT]AGC
2337 GCTCAGC
Bpu1102I 2332 GCTCAGC GC^T[ACGT]AGC
2337 GCTCAGC
BsaAI 547 CACGTG [CT]AC^GT[GA]
607 CACGTG
BsmI 1253 GAATGC GAATGC(1/-1)
3521 GCATTC
Bsp1407I 1061 TGTACA T^GTACA
1617 TGTACA
BsrGI 1061 TGTACA T^GTACA
1617 TGTACA
BstAPI 597 GCACTGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1455 GCAAGGCATGC
CelII 2332 GCTCAGC GC^T[ACGT]AGC
2337 GCTCAGC
DraI 918 TTTAAA TTT^AAA
3731 TTTAAA
FbaI 792 TGATCA T^GATCA
3426 TGATCA
HaeII 129 GGCGCC [GA]GCGC^[CT]
2780 AGCGCT
HgaI 1586 GACGC GACGC(5/10)
1737 GCGTC
PmaCI 547 CACGTG CAC^GTG
607 CACGTG
PmlI 547 CACGTG CAC^GTG
607 CACGTG
PvuII 317 CAGCTG CAG^CTG
3377 CAGCTG
SexAI 2463 ACCTGGT A^CC[AT]GGT
2537 ACCAGGT
SspBI 1061 TGTACA T^GTACA
1617 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DsaI
EaeI EarI EcoNI EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII