##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AcyI AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AspI BbeI BbrPI BcgI BciVI
BclI BglI BsaAI BsaHI BseAI
BsiEI BsiWI BspDI BspEI BspHI
BsrDI BssSI BstXI ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FbaI FseI Hin1I
HpaI KasI MfeI MluI MroI
MunI NarI NcoI NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI PvuI
RcaI RsrII SalI SexAI SfiI
SfoI SgrAI SnaBI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 678 GTATAC GT^[AC][GT]AC
AclI 728 AACGTT AA^CGTT
ApaI 1284 GGGCCC GGGCC^C
AviII 36 TGCGCA TGC^GCA
AvrII 1280 CCTAGG C^CTAGG
BamHI 2602 GGATCC G^GATCC
BbvCI 2036 GCTGAGG CCTCAGC(-5/-2)
BglII 924 AGATCT A^GATCT
BlnI 1280 CCTAGG C^CTAGG
BlpI 8 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 8 GCTGAGC GC^T[ACGT]AGC
BsaBI 2362 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1365 GAGACC GGTCTC(1/5)
BsaWI 2475 ACCGGA [AT]^CCGG[AT]
BsmBI 2464 CGTCTC CGTCTC(1/5)
BsrBI 1549 CCGCTC CCGCTC(-3/-3)
BsrFI 2501 GCCGGC [GA]^CCGG[CT]
Bst1107I 678 GTATAC GTA^TAC
BstBI 1829 TTCGAA TT^CGAA
BstZ17I 678 GTATAC GTA^TAC
CelII 8 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2501 GCCGGC [GA]^CCGG[CT]
DraIII 1102 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1696 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3411 CCTTGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1453 ATGCAT ATGCA^T
FspI 36 TGCGCA TGC^GCA
HaeII 1581 AGCGCC [GA]GCGC^[CT]
HgaI 2463 GCGTC GACGC(5/10)
KspI 2608 CCGCGG CCGC^GG
MamI 2362 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2501 GCCGGC GCC^GGC
NgoMIV 2501 GCCGGC G^CCGGC
NsiI 1453 ATGCAT ATGCA^T
NspV 1829 TTCGAA TT^CGAA
PshAI 2689 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 728 AACGTT AA^CGTT
SacI 1591 GAGCTC GAGCT^C
SacII 2608 CCGCGG CCGC^GG
SbfI 94 CCTGCAGG CCTGCA^GG
ScaI 3333 AGTACT AGT^ACT
SfuI 1829 TTCGAA TT^CGAA
SmaI 1903 CCCGGG CCC^GGG
SpeI 3275 ACTAGT A^CTAGT
SphI 179 GCATGC GCATG^C
Sse8387I 94 CCTGCAGG CCTGCA^GG
StuI 2452 AGGCCT AGG^CCT
SwaI 311 ATTTAAAT ATTT^AAAT
XcmI 116 CCATCTCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1903 CCCGGG C^CCGGG
XmaI 1903 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 244 GGTACC G^GTACC
933 GGTACC
AflII 721 CTTAAG C^TTAAG
2659 CTTAAG
AocI 664 CCTGAGG CC^T[ACGT]AGG
1585 CCTGAGG
Asp700I 726 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3684 GAAGGAGTTC
Asp718I 244 GGTACC G^GTACC
933 GGTACC
AvaI 1903 CCCGGG C^[CT]CG[GA]G
2597 CCCGAG
BalI 1109 TGGCCA TGG^CCA
2403 TGGCCA
BfrI 721 CTTAAG C^TTAAG
2659 CTTAAG
BsgI 2269 CTGCAC GTGCAG(16/14)
1700 GTGCAG
BsoBI 1903 CCCGGG C^[CT]CG[GA]G
2597 CCCGAG
BssHII 851 GCGCGC G^CGCGC
1539 GCGCGC
BstAPI 1601 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2262 GCAACATCTGC
BstEII 547 GGTGACC G^GT[ACGT]ACC
2174 GGTCACC
BstPI 547 GGTGACC G^GT[ACGT]ACC
2174 GGTCACC
Bsu36I 664 CCTGAGG CC^T[ACGT]AGG
1585 CCTGAGG
DraI 312 TTTAAA TTT^AAA
2936 TTTAAA
Eco81I 664 CCTGAGG CC^T[ACGT]AGG
1585 CCTGAGG
EcoO65I 547 GGTGACC G^GT[ACGT]ACC
2174 GGTCACC
HindIII 507 AAGCTT A^AGCTT
1665 AAGCTT
KpnI 244 GGTACC GGTAC^C
933 GGTACC
MluNI 1109 TGGCCA TGG^CCA
2403 TGGCCA
MscI 1109 TGGCCA TGG^CCA
2403 TGGCCA
NdeI 209 CATATG CA^TATG
1450 CATATG
PflMI 1676 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3297 CCAACTATTGG
SapI 2389 GAAGAGC GCTCTTC(1/4)
600 GCTCTTC
TaqI 222 TCGA T^CGA
1830 TCGA
TthHB8I 222 TCGA T^CGA
1830 TCGA
Van91I 1676 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3297 CCAACTATTGG
XmnI 726 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3684 GAAGGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
ApoI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I Bsp1407I BspLU11I BspMI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII