################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII AccIII AcyI AgeI AhdI Aor51HI AscI AseI AsnI AspEI AspI BbeI BbrPI BcgI BciVI BclI BglI BsaAI BsaHI BseAI BsiEI BsiWI BspDI BspEI BspHI BsrDI BssSI BstXI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FbaI FseI Hin1I HpaI KasI MfeI MluI MroI MunI NarI NcoI NheI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI PvuI RcaI RsrII SalI SexAI SfiI SfoI SgrAI SnaBI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 678 GTATAC GT^[AC][GT]AC AclI 728 AACGTT AA^CGTT ApaI 1284 GGGCCC GGGCC^C AviII 36 TGCGCA TGC^GCA AvrII 1280 CCTAGG C^CTAGG BamHI 2602 GGATCC G^GATCC BbvCI 2036 GCTGAGG CCTCAGC(-5/-2) BglII 924 AGATCT A^GATCT BlnI 1280 CCTAGG C^CTAGG BlpI 8 GCTGAGC GC^T[ACGT]AGC Bpu1102I 8 GCTGAGC GC^T[ACGT]AGC BsaBI 2362 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1365 GAGACC GGTCTC(1/5) BsaWI 2475 ACCGGA [AT]^CCGG[AT] BsmBI 2464 CGTCTC CGTCTC(1/5) BsrBI 1549 CCGCTC CCGCTC(-3/-3) BsrFI 2501 GCCGGC [GA]^CCGG[CT] Bst1107I 678 GTATAC GTA^TAC BstBI 1829 TTCGAA TT^CGAA BstZ17I 678 GTATAC GTA^TAC CelII 8 GCTGAGC GC^T[ACGT]AGC Cfr10I 2501 GCCGGC [GA]^CCGG[CT] DraIII 1102 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1696 GACAGTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3411 CCTTGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1453 ATGCAT ATGCA^T FspI 36 TGCGCA TGC^GCA HaeII 1581 AGCGCC [GA]GCGC^[CT] HgaI 2463 GCGTC GACGC(5/10) KspI 2608 CCGCGG CCGC^GG MamI 2362 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2501 GCCGGC GCC^GGC NgoMIV 2501 GCCGGC G^CCGGC NsiI 1453 ATGCAT ATGCA^T NspV 1829 TTCGAA TT^CGAA PshAI 2689 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 728 AACGTT AA^CGTT SacI 1591 GAGCTC GAGCT^C SacII 2608 CCGCGG CCGC^GG SbfI 94 CCTGCAGG CCTGCA^GG ScaI 3333 AGTACT AGT^ACT SfuI 1829 TTCGAA TT^CGAA SmaI 1903 CCCGGG CCC^GGG SpeI 3275 ACTAGT A^CTAGT SphI 179 GCATGC GCATG^C Sse8387I 94 CCTGCAGG CCTGCA^GG StuI 2452 AGGCCT AGG^CCT SwaI 311 ATTTAAAT ATTT^AAAT XcmI 116 CCATCTCCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1903 CCCGGG C^CCGGG XmaI 1903 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 244 GGTACC G^GTACC 933 GGTACC AflII 721 CTTAAG C^TTAAG 2659 CTTAAG AocI 664 CCTGAGG CC^T[ACGT]AGG 1585 CCTGAGG Asp700I 726 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3684 GAAGGAGTTC Asp718I 244 GGTACC G^GTACC 933 GGTACC AvaI 1903 CCCGGG C^[CT]CG[GA]G 2597 CCCGAG BalI 1109 TGGCCA TGG^CCA 2403 TGGCCA BfrI 721 CTTAAG C^TTAAG 2659 CTTAAG BsgI 2269 CTGCAC GTGCAG(16/14) 1700 GTGCAG BsoBI 1903 CCCGGG C^[CT]CG[GA]G 2597 CCCGAG BssHII 851 GCGCGC G^CGCGC 1539 GCGCGC BstAPI 1601 GCAGCCGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2262 GCAACATCTGC BstEII 547 GGTGACC G^GT[ACGT]ACC 2174 GGTCACC BstPI 547 GGTGACC G^GT[ACGT]ACC 2174 GGTCACC Bsu36I 664 CCTGAGG CC^T[ACGT]AGG 1585 CCTGAGG DraI 312 TTTAAA TTT^AAA 2936 TTTAAA Eco81I 664 CCTGAGG CC^T[ACGT]AGG 1585 CCTGAGG EcoO65I 547 GGTGACC G^GT[ACGT]ACC 2174 GGTCACC HindIII 507 AAGCTT A^AGCTT 1665 AAGCTT KpnI 244 GGTACC GGTAC^C 933 GGTACC MluNI 1109 TGGCCA TGG^CCA 2403 TGGCCA MscI 1109 TGGCCA TGG^CCA 2403 TGGCCA NdeI 209 CATATG CA^TATG 1450 CATATG PflMI 1676 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3297 CCAACTATTGG SapI 2389 GAAGAGC GCTCTTC(1/4) 600 GCTCTTC TaqI 222 TCGA T^CGA 1830 TCGA TthHB8I 222 TCGA T^CGA 1830 TCGA Van91I 1676 CCAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3297 CCAACTATTGG XmnI 726 GAAACGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3684 GAAGGAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BspMI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII