################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AgeI Alw44I ApaLI AscI AseI AsnI Asp718I AspI AvrII BamHI BbeI BbrPI BcnI BlnI BsaBI BsaHI BsgI BsiEI BsiWI BsmBI BspDI BsrBI BsrFI BssHII BstBI BstEII BstPI BstUI BstXI Cfr10I ClaI CpoI DraIII EagI EclXI Eco52I EcoO65I EcoRV FseI HgaI Hin1I HincII HindII HpaI KasI KpnI KspI MamI MluI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SpeI SphI Sse8387I SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 190 TCCGGA T^CCGGA AflII 3814 CTTAAG C^TTAAG AhdI 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1788 CCTCAGG CC^T[ACGT]AGG ApaI 2170 GGGCCC GGGCC^C AspEI 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaI 6 CCCGAG C^[CT]CG[GA]G AviII 902 TGCGCA TGC^GCA BalI 1021 TGGCCA TGG^CCA BanI 2345 GGCACC G^G[CT][GA]CC BbvCI 4400 GCTGAGG CCTCAGC(-5/-2) BcgI 2259 CGACATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3814 CTTAAG C^TTAAG BglI 926 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 2701 TACGTA [CT]AC^GT[GA] BseAI 190 TCCGGA T^CCGGA BsmI 1319 GAATGC GAATGC(1/-1) BsoBI 6 CCCGAG C^[CT]CG[GA]G BspEI 190 TCCGGA T^CCGGA BspHI 4294 TCATGA T^CATGA BspMI 1807 ACCTGC ACCTGC(4/8) Bsu36I 1788 CCTCAGG CC^T[ACGT]AGG EaeI 1021 TGGCCA [CT]^GGCC[GA] Eam1105I 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1788 CCTCAGG CC^T[ACGT]AGG EcoT22I 2978 ATGCAT ATGCA^T FauI 16 GCGGG CCCGC(4/6) FspI 902 TGCGCA TGC^GCA MaeII 2702 ACGT A^CGT MfeI 1861 CAATTG C^AATTG MluNI 1021 TGGCCA TGG^CCA MroI 190 TCCGGA T^CCGGA MscI 1021 TGGCCA TGG^CCA MunI 1861 CAATTG C^AATTG NsiI 2978 ATGCAT ATGCA^T PstI 3198 CTGCAG CTGCA^G RcaI 4294 TCATGA T^CATGA SacI 1682 GAGCTC GAGCT^C SnaBI 2701 TACGTA TAC^GTA TaqI 4069 TCGA T^CGA TthHB8I 4069 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwNI 1791 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG 3591 CAGGGGCTG Aor51HI 10 AGCGCT AGC^GCT 3407 AGCGCT BciVI 74 GGATAC GTATCC(6/5) 2146 GTATCC BclI 2250 TGATCA T^GATCA 4610 TGATCA BglII 1663 AGATCT A^GATCT 4057 AGATCT BsrDI 2254 CATTGC GCAATG(2/0) 3531 GCAATG BssSI 791 CACGAG CACGAG(-5/-1) 32 CTCGTG DrdI 420 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3078 GACAGATGGGTC DsaI 1573 CCATGG C^C[GA][CT]GG 2538 CCATGG Eco47III 10 AGCGCT AGC^GCT 3407 AGCGCT FbaI 2250 TGATCA T^GATCA 4610 TGATCA NcoI 1573 CCATGG C^CATGG 2538 CCATGG NdeI 2262 CATATG CA^TATG 3666 CATATG PpuMI 1792 AGGTCCT [GA]G^G[AT]CC[CT] 3440 GGGTCCC SapI 1130 GAAGAGC GCTCTTC(1/4) 3630 GCTCTTC StuI 741 AGGCCT AGG^CCT 3148 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI Bst1107I BstAPI BstF5I BstNI BstYI BstZ17I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmnI