##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AgeI Alw44I ApaLI AscI AseI
AsnI Asp718I AspI AvrII BamHI
BbeI BbrPI BcnI BlnI BsaBI
BsaHI BsgI BsiEI BsiWI BsmBI
BspDI BsrBI BsrFI BssHII BstBI
BstEII BstPI BstUI BstXI Cfr10I
ClaI CpoI DraIII EagI EclXI
Eco52I EcoO65I EcoRV FseI HgaI
Hin1I HincII HindII HpaI KasI
KpnI KspI MamI MluI MvnI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 190 TCCGGA T^CCGGA
AflII 3814 CTTAAG C^TTAAG
AhdI 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1788 CCTCAGG CC^T[ACGT]AGG
ApaI 2170 GGGCCC GGGCC^C
AspEI 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 6 CCCGAG C^[CT]CG[GA]G
AviII 902 TGCGCA TGC^GCA
BalI 1021 TGGCCA TGG^CCA
BanI 2345 GGCACC G^G[CT][GA]CC
BbvCI 4400 GCTGAGG CCTCAGC(-5/-2)
BcgI 2259 CGACATATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3814 CTTAAG C^TTAAG
BglI 926 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2701 TACGTA [CT]AC^GT[GA]
BseAI 190 TCCGGA T^CCGGA
BsmI 1319 GAATGC GAATGC(1/-1)
BsoBI 6 CCCGAG C^[CT]CG[GA]G
BspEI 190 TCCGGA T^CCGGA
BspHI 4294 TCATGA T^CATGA
BspMI 1807 ACCTGC ACCTGC(4/8)
Bsu36I 1788 CCTCAGG CC^T[ACGT]AGG
EaeI 1021 TGGCCA [CT]^GGCC[GA]
Eam1105I 2932 GACTAATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1788 CCTCAGG CC^T[ACGT]AGG
EcoT22I 2978 ATGCAT ATGCA^T
FauI 16 GCGGG CCCGC(4/6)
FspI 902 TGCGCA TGC^GCA
MaeII 2702 ACGT A^CGT
MfeI 1861 CAATTG C^AATTG
MluNI 1021 TGGCCA TGG^CCA
MroI 190 TCCGGA T^CCGGA
MscI 1021 TGGCCA TGG^CCA
MunI 1861 CAATTG C^AATTG
NsiI 2978 ATGCAT ATGCA^T
PstI 3198 CTGCAG CTGCA^G
RcaI 4294 TCATGA T^CATGA
SacI 1682 GAGCTC GAGCT^C
SnaBI 2701 TACGTA TAC^GTA
TaqI 4069 TCGA T^CGA
TthHB8I 4069 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AlwNI 1791 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
3591 CAGGGGCTG
Aor51HI 10 AGCGCT AGC^GCT
3407 AGCGCT
BciVI 74 GGATAC GTATCC(6/5)
2146 GTATCC
BclI 2250 TGATCA T^GATCA
4610 TGATCA
BglII 1663 AGATCT A^GATCT
4057 AGATCT
BsrDI 2254 CATTGC GCAATG(2/0)
3531 GCAATG
BssSI 791 CACGAG CACGAG(-5/-1)
32 CTCGTG
DrdI 420 GACAGATGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3078 GACAGATGGGTC
DsaI 1573 CCATGG C^C[GA][CT]GG
2538 CCATGG
Eco47III 10 AGCGCT AGC^GCT
3407 AGCGCT
FbaI 2250 TGATCA T^GATCA
4610 TGATCA
NcoI 1573 CCATGG C^CATGG
2538 CCATGG
NdeI 2262 CATATG CA^TATG
3666 CATATG
PpuMI 1792 AGGTCCT [GA]G^G[AT]CC[CT]
3440 GGGTCCC
SapI 1130 GAAGAGC GCTCTTC(1/4)
3630 GCTCTTC
StuI 741 AGGCCT AGG^CCT
3148 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI ApoI Asp700I AspHI
AvaII BanII BbsI BbvI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspLU11I BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstYI
BstZ17I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraI DraII EarI
EcoNI EcoO109I EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII XmnI