##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AclI        AflII       AhdI        AocI        AscI        
AseI        AsnI        AspEI       AviII       AvrII       
BciVI       BfrI        BglII       BlnI        BsaBI       
BsmI        Bsp1407I    BspHI       BspLU11I    BsrGI       
BssSI       Bst1107I    BstBI       BstZ17I     Bsu36I      
CpoI        DraI        Eam1105I    Eco81I      EcoRI       
EcoRV       EcoT22I     FspI        HincII      HindII      
HindIII     HpaI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PmeI        
PshBI       Psp1406I    RcaI        RsrII       SalI        
SbfI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       Sse8387I    SspBI       SspI        
SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   24 enzymes) #
###################################

AccI         1029  GTCTAC               GT^[AC][GT]AC
AflIII       3829  ACGTGT               A^C[GA][CT]GT
AgeI          832  ACCGGT               A^CCGGT
AspI          978  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI        3199  CACGTG               CAC^GTG
BcgI         3787  CGATATGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2584  TGATCA               T^GATCA
BsiWI         850  CGTACG               C^GTACG
BspDI        1134  ATCGAT               AT^CGAT
BsrDI        2024  CATTGC               GCAATG(2/0)
BstAPI       2768  GCAGCTGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        798  GGTAACC              G^GT[ACGT]ACC
BstPI         798  GGTAACC              G^GT[ACGT]ACC
ClaI         1134  ATCGAT               AT^CGAT
EcoO65I       798  GGTAACC              G^GT[ACGT]ACC
FbaI         2584  TGATCA               T^GATCA
PflFI         978  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PinAI         832  ACCGGT               A^CCGGT
PmaCI        3199  CACGTG               CAC^GTG
PmlI         3199  CACGTG               CAC^GTG
PvuI          765  CGATCG               CGAT^CG
SpeI         1899  ACTAGT               A^CTAGT
Tth111I       978  GACGCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         2470  CCAGTTTGAACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

Acc65I        770  GGTACC               G^GTACC
              835  GGTACC              
AccIII       1486  TCCGGA               T^CCGGA
             3349  TCCGGA              
ApaI           61  GGGCCC               GGGCC^C
              944  GGGCCC              
Asp700I      2720  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3332  GAACTCTTTC          
Asp718I       770  GGTACC               G^GTACC
              835  GGTACC              
BbvCI         367  CCTCAGC              CCTCAGC(-5/-2)
               56  GCTGAGG             
BlpI         2919  GCTCAGC              GC^T[ACGT]AGC
             3141  GCTCAGC             
Bpu1102I     2919  GCTCAGC              GC^T[ACGT]AGC
             3141  GCTCAGC             
BseAI        1486  TCCGGA               T^CCGGA
             3349  TCCGGA              
BspEI        1486  TCCGGA               T^CCGGA
             3349  TCCGGA              
BssHII         96  GCGCGC               G^CGCGC
              537  GCGCGC              
CelII        2919  GCTCAGC              GC^T[ACGT]AGC
             3141  GCTCAGC             
DrdI         2013  GACGCTACAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2295  GACGCATGGGTC        
EcoNI        1847  CCTGGCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3222  CCTCTTCCAGG         
FseI          204  GGCCGGCC             GGCCGG^CC
              271  GGCCGGCC            
KpnI          770  GGTACC               GGTAC^C
              835  GGTACC              
MroI         1486  TCCGGA               T^CCGGA
             3349  TCCGGA              
PflMI         870  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3839  CCAGCCCTTGG         
PshAI         324  GACGCTGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             3938  GACTTCAGTC          
SacI          777  GAGCTC               GAGCT^C
             3139  GAGCTC              
SapI         2208  GAAGAGC              GCTCTTC(1/4)
             3089  GAAGAGC             
SmlI         1760  CTTGAG               C^T[CT][GA]AG
             3295  CTCAAG              
SphI         1618  GCATGC               GCATG^C
             1966  GCATGC              
StuI         1559  AGGCCT               AGG^CCT
             2998  AGGCCT              
Van91I        870  CCAGAAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3839  CCAGCCCTTGG         
XmnI         2720  GAAACTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3332  GAACTCTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  140 enzymes) #
#########################################

AatII       AccII       AciI        AcsI        AcyI        
AfaI        AluI        Alw44I      AlwI        AlwNI       
Aor51HI     ApaLI       ApoI        AspHI       AvaI        
AvaII       BalI        BamHI       BanI        BanII       
BbeI        BbsI        BbvI        BcnI        BfaI        
BglI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaI        BsaJI       BsaWI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsoBI       Bsp1286I    
BspMI       BsrBI       BsrFI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EaeI        
EagI        EarI        EclXI       Eco47III    Eco52I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KasI        Ksp632I     KspI        
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeII       NgoMIV      NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SacII       Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        SmaI        StyI        TaqI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     XhoII       XmaCI       XmaI