##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AclI AflII AhdI AocI AscI
AseI AsnI AspEI AviII AvrII
BciVI BfrI BglII BlnI BsaBI
BsmI Bsp1407I BspHI BspLU11I BsrGI
BssSI Bst1107I BstBI BstZ17I Bsu36I
CpoI DraI Eam1105I Eco81I EcoRI
EcoRV EcoT22I FspI HincII HindII
HindIII HpaI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PshBI Psp1406I RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SnaBI Sse8387I SspBI SspI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 24 enzymes) #
###################################
AccI 1029 GTCTAC GT^[AC][GT]AC
AflIII 3829 ACGTGT A^C[GA][CT]GT
AgeI 832 ACCGGT A^CCGGT
AspI 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 3199 CACGTG CAC^GTG
BcgI 3787 CGATATGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2584 TGATCA T^GATCA
BsiWI 850 CGTACG C^GTACG
BspDI 1134 ATCGAT AT^CGAT
BsrDI 2024 CATTGC GCAATG(2/0)
BstAPI 2768 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 798 GGTAACC G^GT[ACGT]ACC
BstPI 798 GGTAACC G^GT[ACGT]ACC
ClaI 1134 ATCGAT AT^CGAT
EcoO65I 798 GGTAACC G^GT[ACGT]ACC
FbaI 2584 TGATCA T^GATCA
PflFI 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 832 ACCGGT A^CCGGT
PmaCI 3199 CACGTG CAC^GTG
PmlI 3199 CACGTG CAC^GTG
PvuI 765 CGATCG CGAT^CG
SpeI 1899 ACTAGT A^CTAGT
Tth111I 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2470 CCAGTTTGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
Acc65I 770 GGTACC G^GTACC
835 GGTACC
AccIII 1486 TCCGGA T^CCGGA
3349 TCCGGA
ApaI 61 GGGCCC GGGCC^C
944 GGGCCC
Asp700I 2720 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3332 GAACTCTTTC
Asp718I 770 GGTACC G^GTACC
835 GGTACC
BbvCI 367 CCTCAGC CCTCAGC(-5/-2)
56 GCTGAGG
BlpI 2919 GCTCAGC GC^T[ACGT]AGC
3141 GCTCAGC
Bpu1102I 2919 GCTCAGC GC^T[ACGT]AGC
3141 GCTCAGC
BseAI 1486 TCCGGA T^CCGGA
3349 TCCGGA
BspEI 1486 TCCGGA T^CCGGA
3349 TCCGGA
BssHII 96 GCGCGC G^CGCGC
537 GCGCGC
CelII 2919 GCTCAGC GC^T[ACGT]AGC
3141 GCTCAGC
DrdI 2013 GACGCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2295 GACGCATGGGTC
EcoNI 1847 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3222 CCTCTTCCAGG
FseI 204 GGCCGGCC GGCCGG^CC
271 GGCCGGCC
KpnI 770 GGTACC GGTAC^C
835 GGTACC
MroI 1486 TCCGGA T^CCGGA
3349 TCCGGA
PflMI 870 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3839 CCAGCCCTTGG
PshAI 324 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3938 GACTTCAGTC
SacI 777 GAGCTC GAGCT^C
3139 GAGCTC
SapI 2208 GAAGAGC GCTCTTC(1/4)
3089 GAAGAGC
SmlI 1760 CTTGAG C^T[CT][GA]AG
3295 CTCAAG
SphI 1618 GCATGC GCATG^C
1966 GCATGC
StuI 1559 AGGCCT AGG^CCT
2998 AGGCCT
Van91I 870 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3839 CCAGCCCTTGG
XmnI 2720 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3332 GAACTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 140 enzymes) #
#########################################
AatII AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
Aor51HI ApaLI ApoI AspHI AvaI
AvaII BalI BamHI BanI BanII
BbeI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EagI EarI EclXI Eco47III Eco52I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NcoI
NdeII NgoMIV NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmaCI XmaI