################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AclI AflII AhdI AocI AscI AseI AsnI AspEI AviII AvrII BciVI BfrI BglII BlnI BsaBI BsmI Bsp1407I BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I CpoI DraI Eam1105I Eco81I EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PmeI PshBI Psp1406I RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 24 enzymes) # ################################### AccI 1029 GTCTAC GT^[AC][GT]AC AflIII 3829 ACGTGT A^C[GA][CT]GT AgeI 832 ACCGGT A^CCGGT AspI 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 3199 CACGTG CAC^GTG BcgI 3787 CGATATGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2584 TGATCA T^GATCA BsiWI 850 CGTACG C^GTACG BspDI 1134 ATCGAT AT^CGAT BsrDI 2024 CATTGC GCAATG(2/0) BstAPI 2768 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 798 GGTAACC G^GT[ACGT]ACC BstPI 798 GGTAACC G^GT[ACGT]ACC ClaI 1134 ATCGAT AT^CGAT EcoO65I 798 GGTAACC G^GT[ACGT]ACC FbaI 2584 TGATCA T^GATCA PflFI 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 832 ACCGGT A^CCGGT PmaCI 3199 CACGTG CAC^GTG PmlI 3199 CACGTG CAC^GTG PvuI 765 CGATCG CGAT^CG SpeI 1899 ACTAGT A^CTAGT Tth111I 978 GACGCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2470 CCAGTTTGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Acc65I 770 GGTACC G^GTACC 835 GGTACC AccIII 1486 TCCGGA T^CCGGA 3349 TCCGGA ApaI 61 GGGCCC GGGCC^C 944 GGGCCC Asp700I 2720 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3332 GAACTCTTTC Asp718I 770 GGTACC G^GTACC 835 GGTACC BbvCI 367 CCTCAGC CCTCAGC(-5/-2) 56 GCTGAGG BlpI 2919 GCTCAGC GC^T[ACGT]AGC 3141 GCTCAGC Bpu1102I 2919 GCTCAGC GC^T[ACGT]AGC 3141 GCTCAGC BseAI 1486 TCCGGA T^CCGGA 3349 TCCGGA BspEI 1486 TCCGGA T^CCGGA 3349 TCCGGA BssHII 96 GCGCGC G^CGCGC 537 GCGCGC CelII 2919 GCTCAGC GC^T[ACGT]AGC 3141 GCTCAGC DrdI 2013 GACGCTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2295 GACGCATGGGTC EcoNI 1847 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3222 CCTCTTCCAGG FseI 204 GGCCGGCC GGCCGG^CC 271 GGCCGGCC KpnI 770 GGTACC GGTAC^C 835 GGTACC MroI 1486 TCCGGA T^CCGGA 3349 TCCGGA PflMI 870 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3839 CCAGCCCTTGG PshAI 324 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3938 GACTTCAGTC SacI 777 GAGCTC GAGCT^C 3139 GAGCTC SapI 2208 GAAGAGC GCTCTTC(1/4) 3089 GAAGAGC SmlI 1760 CTTGAG C^T[CT][GA]AG 3295 CTCAAG SphI 1618 GCATGC GCATG^C 1966 GCATGC StuI 1559 AGGCCT AGG^CCT 2998 AGGCCT Van91I 870 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3839 CCAGCCCTTGG XmnI 2720 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3332 GAACTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AatII AccII AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI Aor51HI ApaLI ApoI AspHI AvaI AvaII BalI BamHI BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco47III Eco52I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmaCI XmaI