################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI AspEI AvrII BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI Eam1105I Eco47III EcoO65I EcoRI EcoRV FbaI FseI HindIII HpaI KspI MfeI MluI MunI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI Tsp509I XbaI XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### Acc65I 2070 GGTACC G^GTACC AccI 2918 GTAGAC GT^[AC][GT]AC AccIII 1022 TCCGGA T^CCGGA Alw44I 2042 GTGCAC G^TGCAC AocI 1873 CCTGAGG CC^T[ACGT]AGG ApaLI 2042 GTGCAC G^TGCAC Asp700I 3690 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2070 GGTACC G^GTACC AspI 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2091 GGATCC G^GATCC BsaBI 1732 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 1022 TCCGGA T^CCGGA BsgI 1896 GTGCAG GTGCAG(16/14) BsmBI 2288 CGTCTC CGTCTC(1/5) BsmI 310 GCATTC GAATGC(1/-1) BspEI 1022 TCCGGA T^CCGGA Bsu36I 1873 CCTGAGG CC^T[ACGT]AGG DraI 3841 TTTAAA TTT^AAA DraIII 1403 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1873 CCTGAGG CC^T[ACGT]AGG EcoT22I 3865 ATGCAT ATGCA^T KpnI 2070 GGTACC GGTAC^C MamI 1732 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 1022 TCCGGA T^CCGGA NaeI 3793 GCCGGC GCC^GGC NdeI 1071 CATATG CA^TATG NgoMIV 3793 GCCGGC G^CCGGC NotI 1035 GCGGCCGC GC^GGCCGC NsiI 3865 ATGCAT ATGCA^T PflFI 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 1885 GAGCTC GAGCT^C SapI 1235 GAAGAGC GCTCTTC(1/4) SbfI 2686 CCTGCAGG CCTGCA^GG SexAI 961 ACCTGGT A^CC[AT]GGT SphI 3185 GCATGC GCATG^C Sse8387I 2686 CCTGCAGG CCTGCA^GG Tth111I 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 3690 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 612 ACGTGT A^C[GA][CT]GT 2764 ACACGT BbeI 393 GGCGCC GGCGC^C 1548 GGCGCC BbvCI 3155 CCTCAGC CCTCAGC(-5/-2) 1538 GCTGAGG BsaI 2456 GAGACC GGTCTC(1/5) 3070 GAGACC BsiEI 315 CGGCCG CG[GA][CT]^CG 1036 CGGCCG BspMI 1440 ACCTGC ACCTGC(4/8) 2226 ACCTGC BsrDI 2571 GCAATG GCAATG(2/0) 3498 GCAATG BssHII 214 GCGCGC G^CGCGC 2473 GCGCGC BstYI 259 AGATCC [GA]^GATC[CT] 2091 GGATCC DrdI 1667 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3167 GACTCCTGGGTC EagI 315 CGGCCG C^GGCCG 1036 CGGCCG EclXI 315 CGGCCG C^GGCCG 1036 CGGCCG Eco52I 315 CGGCCG C^GGCCG 1036 CGGCCG HincII 3002 GTTGAC GT[CT]^[GA]AC 3164 GTTGAC HindII 3002 GTTGAC GT[CT]^[GA]AC 3164 GTTGAC KasI 393 GGCGCC G^GCGCC 1548 GGCGCC MflI 259 AGATCC [GA]^GATC[CT] 2091 GGATCC MslI 845 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2591 CACCCCAGTG NarI 393 GGCGCC GG^CGCC 1548 GGCGCC NcoI 1008 CCATGG C^CATGG 2967 CCATGG NheI 24 GCTAGC G^CTAGC 2849 GCTAGC ScaI 2431 AGTACT AGT^ACT 3554 AGTACT SfiI 944 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 3105 GGCCCAGCAGGCC SfoI 393 GGCGCC GGC^GCC 1548 GGCGCC SmlI 3265 CTTGAG C^T[CT][GA]AG 3769 CTCAAG TfiI 1019 GAATC G^A[AT]TC 3682 GATTC XcmI 2544 CCATGACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2595 CCAGTGCCCACCTGG XhoII 259 AGATCC [GA]^GATC[CT] 2091 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII AviII BalI BanI BanII BbrPI BbsI BbvI BciVI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TaqI Tru9I TseI Tsp45I TspRI TthHB8I Van91I XmaCI XmaI