##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AclI AcsI AflII AgeI
AhdI Aor51HI ApoI AscI AseI
AsnI AspEI AvrII BcgI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrGI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CelII ClaI CpoI
Eam1105I Eco47III EcoO65I EcoRI EcoRV
FbaI FseI HindIII HpaI KspI
MfeI MluI MunI NruI NspV
PacI PaeR7I PinAI PmeI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SfuI SgrAI SnaBI SpeI
SspBI SspI StuI SwaI Tsp509I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 2070 GGTACC G^GTACC
AccI 2918 GTAGAC GT^[AC][GT]AC
AccIII 1022 TCCGGA T^CCGGA
Alw44I 2042 GTGCAC G^TGCAC
AocI 1873 CCTGAGG CC^T[ACGT]AGG
ApaLI 2042 GTGCAC G^TGCAC
Asp700I 3690 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2070 GGTACC G^GTACC
AspI 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2091 GGATCC G^GATCC
BsaBI 1732 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1022 TCCGGA T^CCGGA
BsgI 1896 GTGCAG GTGCAG(16/14)
BsmBI 2288 CGTCTC CGTCTC(1/5)
BsmI 310 GCATTC GAATGC(1/-1)
BspEI 1022 TCCGGA T^CCGGA
Bsu36I 1873 CCTGAGG CC^T[ACGT]AGG
DraI 3841 TTTAAA TTT^AAA
DraIII 1403 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1873 CCTGAGG CC^T[ACGT]AGG
EcoT22I 3865 ATGCAT ATGCA^T
KpnI 2070 GGTACC GGTAC^C
MamI 1732 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1022 TCCGGA T^CCGGA
NaeI 3793 GCCGGC GCC^GGC
NdeI 1071 CATATG CA^TATG
NgoMIV 3793 GCCGGC G^CCGGC
NotI 1035 GCGGCCGC GC^GGCCGC
NsiI 3865 ATGCAT ATGCA^T
PflFI 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 1885 GAGCTC GAGCT^C
SapI 1235 GAAGAGC GCTCTTC(1/4)
SbfI 2686 CCTGCAGG CCTGCA^GG
SexAI 961 ACCTGGT A^CC[AT]GGT
SphI 3185 GCATGC GCATG^C
Sse8387I 2686 CCTGCAGG CCTGCA^GG
Tth111I 2827 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 3690 GAACACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 612 ACGTGT A^C[GA][CT]GT
2764 ACACGT
BbeI 393 GGCGCC GGCGC^C
1548 GGCGCC
BbvCI 3155 CCTCAGC CCTCAGC(-5/-2)
1538 GCTGAGG
BsaI 2456 GAGACC GGTCTC(1/5)
3070 GAGACC
BsiEI 315 CGGCCG CG[GA][CT]^CG
1036 CGGCCG
BspMI 1440 ACCTGC ACCTGC(4/8)
2226 ACCTGC
BsrDI 2571 GCAATG GCAATG(2/0)
3498 GCAATG
BssHII 214 GCGCGC G^CGCGC
2473 GCGCGC
BstYI 259 AGATCC [GA]^GATC[CT]
2091 GGATCC
DrdI 1667 GACAATGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3167 GACTCCTGGGTC
EagI 315 CGGCCG C^GGCCG
1036 CGGCCG
EclXI 315 CGGCCG C^GGCCG
1036 CGGCCG
Eco52I 315 CGGCCG C^GGCCG
1036 CGGCCG
HincII 3002 GTTGAC GT[CT]^[GA]AC
3164 GTTGAC
HindII 3002 GTTGAC GT[CT]^[GA]AC
3164 GTTGAC
KasI 393 GGCGCC G^GCGCC
1548 GGCGCC
MflI 259 AGATCC [GA]^GATC[CT]
2091 GGATCC
MslI 845 CACAGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2591 CACCCCAGTG
NarI 393 GGCGCC GG^CGCC
1548 GGCGCC
NcoI 1008 CCATGG C^CATGG
2967 CCATGG
NheI 24 GCTAGC G^CTAGC
2849 GCTAGC
ScaI 2431 AGTACT AGT^ACT
3554 AGTACT
SfiI 944 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3105 GGCCCAGCAGGCC
SfoI 393 GGCGCC GGC^GCC
1548 GGCGCC
SmlI 3265 CTTGAG C^T[CT][GA]AG
3769 CTCAAG
TfiI 1019 GAATC G^A[AT]TC
3682 GATTC
XcmI 2544 CCATGACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2595 CCAGTGCCCACCTGG
XhoII 259 AGATCC [GA]^GATC[CT]
2091 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbrPI BbsI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XmaCI XmaI