##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AspEI AviII BamHI BbvCI BcgI
BglI BsaBI BseAI BsiWI BsmBI
BspEI BssHII BssSI BstBI BstEII
BstPI Bsu36I CpoI DraIII Eam1105I
Eco47III Eco81I EcoO65I EcoRV EcoT22I
FseI FspI HpaI KspI MamI
MluI MroI NdeI NotI NsiI
NspV PacI PaeR7I PinAI PmeI
Psp1406I PvuI RsrII SacII SbfI
SexAI SfiI SfuI SgrAI SnaBI
Sse8387I SwaI XbaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 682 GGTACC G^GTACC
AflII 1277 CTTAAG C^TTAAG
ApaI 587 GGGCCC GGGCC^C
Asp700I 2243 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 682 GGTACC G^GTACC
AspI 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1706 CACGTG CAC^GTG
BciVI 793 GGATAC GTATCC(6/5)
BclI 1894 TGATCA T^GATCA
BfrI 1277 CTTAAG C^TTAAG
BglII 483 AGATCT A^GATCT
BsaAI 1706 CACGTG [CT]AC^GT[GA]
BsaI 5014 GGTCTC GGTCTC(1/5)
BsgI 738 CTGCAC GTGCAG(16/14)
BsiEI 855 CGGCCG CG[GA][CT]^CG
Bsp1407I 2294 TGTACA T^GTACA
BspDI 1343 ATCGAT AT^CGAT
BsrBI 505 CCGCTC CCGCTC(-3/-3)
BsrDI 2298 CATTGC GCAATG(2/0)
BsrGI 2294 TGTACA T^GTACA
Bst1107I 3001 GTATAC GTA^TAC
BstZ17I 3001 GTATAC GTA^TAC
ClaI 1343 ATCGAT AT^CGAT
DrdI 4041 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 855 CGGCCG C^GGCCG
EclXI 855 CGGCCG C^GGCCG
Eco52I 855 CGGCCG C^GGCCG
EcoNI 2028 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2717 GAATTC G^AATTC
FbaI 1894 TGATCA T^GATCA
HgaI 863 GCGTC GACGC(5/10)
KpnI 682 GGTACC GGTAC^C
MaeII 1707 ACGT A^CGT
MfeI 3675 CAATTG C^AATTG
MunI 3675 CAATTG C^AATTG
NaeI 1325 GCCGGC GCC^GGC
NgoMIV 1325 GCCGGC G^CCGGC
NruI 1006 TCGCGA TCG^CGA
PflFI 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 571 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1706 CACGTG CAC^GTG
PmlI 1706 CACGTG CAC^GTG
PshAI 3755 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 75 GAGCTC GAGCT^C
SalI 865 GTCGAC G^TCGAC
SapI 908 GAAGAGC GCTCTTC(1/4)
SpeI 3976 ACTAGT A^CTAGT
SphI 2079 GCATGC GCATG^C
SspBI 2294 TGTACA T^GTACA
StuI 3282 AGGCCT AGG^CCT
Tth111I 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 571 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 2243 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 146 GGCGCC G[GA]^CG[CT]C
520 GGCGCC
AvrII 307 CCTAGG C^CTAGG
3045 CCTAGG
BalI 634 TGGCCA TGG^CCA
5132 TGGCCA
BbeI 146 GGCGCC GGCGC^C
520 GGCGCC
BbsI 1082 GAAGAC GAAGAC(2/6)
2234 GAAGAC
BlnI 307 CCTAGG C^CTAGG
3045 CCTAGG
BpuAI 1082 GAAGAC GAAGAC(2/6)
2234 GAAGAC
BsaHI 146 GGCGCC G[GA]^CG[CT]C
520 GGCGCC
BsaWI 85 TCCGGT [AT]^CCGG[AT]
1989 ACCGGA
BspHI 2272 TCATGA T^CATGA
2440 TCATGA
BspLU11I 4402 ACATGT A^CATGT
4930 ACATGT
BspMI 779 ACCTGC ACCTGC(4/8)
602 GCAGGT
BstAPI 360 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1329 GCAGCCGCTGC
BstXI 873 CCACGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1465 CCATGCCAGTGG
Hin1I 146 GGCGCC G[GA]^CG[CT]C
520 GGCGCC
HindIII 3057 AAGCTT A^AGCTT
4678 AAGCTT
KasI 146 GGCGCC G^GCGCC
520 GGCGCC
MluNI 634 TGGCCA TGG^CCA
5132 TGGCCA
MscI 634 TGGCCA TGG^CCA
5132 TGGCCA
NarI 146 GGCGCC GG^CGCC
520 GGCGCC
NcoI 531 CCATGG C^CATGG
4618 CCATGG
RcaI 2272 TCATGA T^CATGA
2440 TCATGA
ScaI 1436 AGTACT AGT^ACT
4414 AGTACT
SfoI 146 GGCGCC GGC^GCC
520 GGCGCC
SmaI 151 CCCGGG CCC^GGG
1152 CCCGGG
TaqI 866 TCGA T^CGA
1344 TCGA
TthHB8I 866 TCGA T^CGA
1344 TCGA
XmaCI 151 CCCGGG C^CCGGG
1152 CCCGGG
XmaI 151 CCCGGG C^CCGGG
1152 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI ApoI
AseI AsnI AspHI AvaI AvaII
BanI BanII BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NheI NlaIII NlaIV NspI
PleI PpuMI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII