################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AspEI AviII BamHI BbvCI BcgI BglI BsaBI BseAI BsiWI BsmBI BspEI BssHII BssSI BstBI BstEII BstPI Bsu36I CpoI DraIII Eam1105I Eco47III Eco81I EcoO65I EcoRV EcoT22I FseI FspI HpaI KspI MamI MluI MroI NdeI NotI NsiI NspV PacI PaeR7I PinAI PmeI Psp1406I PvuI RsrII SacII SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### Acc65I 682 GGTACC G^GTACC AflII 1277 CTTAAG C^TTAAG ApaI 587 GGGCCC GGGCC^C Asp700I 2243 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 682 GGTACC G^GTACC AspI 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 1706 CACGTG CAC^GTG BciVI 793 GGATAC GTATCC(6/5) BclI 1894 TGATCA T^GATCA BfrI 1277 CTTAAG C^TTAAG BglII 483 AGATCT A^GATCT BsaAI 1706 CACGTG [CT]AC^GT[GA] BsaI 5014 GGTCTC GGTCTC(1/5) BsgI 738 CTGCAC GTGCAG(16/14) BsiEI 855 CGGCCG CG[GA][CT]^CG Bsp1407I 2294 TGTACA T^GTACA BspDI 1343 ATCGAT AT^CGAT BsrBI 505 CCGCTC CCGCTC(-3/-3) BsrDI 2298 CATTGC GCAATG(2/0) BsrGI 2294 TGTACA T^GTACA Bst1107I 3001 GTATAC GTA^TAC BstZ17I 3001 GTATAC GTA^TAC ClaI 1343 ATCGAT AT^CGAT DrdI 4041 GACTTAATAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 855 CGGCCG C^GGCCG EclXI 855 CGGCCG C^GGCCG Eco52I 855 CGGCCG C^GGCCG EcoNI 2028 CCTATGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2717 GAATTC G^AATTC FbaI 1894 TGATCA T^GATCA HgaI 863 GCGTC GACGC(5/10) KpnI 682 GGTACC GGTAC^C MaeII 1707 ACGT A^CGT MfeI 3675 CAATTG C^AATTG MunI 3675 CAATTG C^AATTG NaeI 1325 GCCGGC GCC^GGC NgoMIV 1325 GCCGGC G^CCGGC NruI 1006 TCGCGA TCG^CGA PflFI 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 571 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1706 CACGTG CAC^GTG PmlI 1706 CACGTG CAC^GTG PshAI 3755 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 75 GAGCTC GAGCT^C SalI 865 GTCGAC G^TCGAC SapI 908 GAAGAGC GCTCTTC(1/4) SpeI 3976 ACTAGT A^CTAGT SphI 2079 GCATGC GCATG^C SspBI 2294 TGTACA T^GTACA StuI 3282 AGGCCT AGG^CCT Tth111I 2962 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 571 CCAGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2243 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcyI 146 GGCGCC G[GA]^CG[CT]C 520 GGCGCC AvrII 307 CCTAGG C^CTAGG 3045 CCTAGG BalI 634 TGGCCA TGG^CCA 5132 TGGCCA BbeI 146 GGCGCC GGCGC^C 520 GGCGCC BbsI 1082 GAAGAC GAAGAC(2/6) 2234 GAAGAC BlnI 307 CCTAGG C^CTAGG 3045 CCTAGG BpuAI 1082 GAAGAC GAAGAC(2/6) 2234 GAAGAC BsaHI 146 GGCGCC G[GA]^CG[CT]C 520 GGCGCC BsaWI 85 TCCGGT [AT]^CCGG[AT] 1989 ACCGGA BspHI 2272 TCATGA T^CATGA 2440 TCATGA BspLU11I 4402 ACATGT A^CATGT 4930 ACATGT BspMI 779 ACCTGC ACCTGC(4/8) 602 GCAGGT BstAPI 360 GCATCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1329 GCAGCCGCTGC BstXI 873 CCACGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1465 CCATGCCAGTGG Hin1I 146 GGCGCC G[GA]^CG[CT]C 520 GGCGCC HindIII 3057 AAGCTT A^AGCTT 4678 AAGCTT KasI 146 GGCGCC G^GCGCC 520 GGCGCC MluNI 634 TGGCCA TGG^CCA 5132 TGGCCA MscI 634 TGGCCA TGG^CCA 5132 TGGCCA NarI 146 GGCGCC GG^CGCC 520 GGCGCC NcoI 531 CCATGG C^CATGG 4618 CCATGG RcaI 2272 TCATGA T^CATGA 2440 TCATGA ScaI 1436 AGTACT AGT^ACT 4414 AGTACT SfoI 146 GGCGCC GGC^GCC 520 GGCGCC SmaI 151 CCCGGG CCC^GGG 1152 CCCGGG TaqI 866 TCGA T^CGA 1344 TCGA TthHB8I 866 TCGA T^CGA 1344 TCGA XmaCI 151 CCCGGG C^CCGGG 1152 CCCGGG XmaI 151 CCCGGG C^CCGGG 1152 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BanI BanII BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII