##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflIII AgeI AhdI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII BbrPI BclI
BsaAI BsaI BsiEI BsiWI Bsp1407I
BspDI BspLU11I BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI KspI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AflII 3088 CTTAAG C^TTAAG
Alw44I 1440 GTGCAC G^TGCAC
Aor51HI 1617 AGCGCT AGC^GCT
ApaLI 1440 GTGCAC G^TGCAC
AvrII 2423 CCTAGG C^CTAGG
BamHI 2103 GGATCC G^GATCC
BbvCI 656 GCTGAGG CCTCAGC(-5/-2)
BciVI 2927 GTATCC GTATCC(6/5)
BfrI 3088 CTTAAG C^TTAAG
BlnI 2423 CCTAGG C^CTAGG
BsaBI 850 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1376 GAATGC GAATGC(1/-1)
BspHI 1956 TCATGA T^CATGA
BsrDI 1933 CATTGC GCAATG(2/0)
BsrFI 1121 GCCGGC [GA]^CCGG[CT]
Cfr10I 1121 GCCGGC [GA]^CCGG[CT]
DraI 154 TTTAAA TTT^AAA
Eco47III 1617 AGCGCT AGC^GCT
EcoRV 1754 GATATC GAT^ATC
HincII 2363 GTTGAC GT[CT]^[GA]AC
HindII 2363 GTTGAC GT[CT]^[GA]AC
MamI 850 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 1121 GCCGGC GCC^GGC
NgoMIV 1121 GCCGGC G^CCGGC
PshAI 1783 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1956 TCATGA T^CATGA
SacI 2578 GAGCTC GAGCT^C
SfiI 2729 GGCCCCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 63 AGGCCT AGG^CCT
Tsp509I 2331 AATT ^AATT
XcmI 2923 CCATGTATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccIII 1971 TCCGGA T^CCGGA
2100 TCCGGA
BglI 1048 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2730 GCCCCAAGGGC
BglII 697 AGATCT A^GATCT
828 AGATCT
BseAI 1971 TCCGGA T^CCGGA
2100 TCCGGA
BsmBI 255 CGTCTC CGTCTC(1/5)
346 CGTCTC
BspEI 1971 TCCGGA T^CCGGA
2100 TCCGGA
BssHII 1859 GCGCGC G^CGCGC
1861 GCGCGC
BstXI 753 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2464 CCATGGAGATGG
MroI 1971 TCCGGA T^CCGGA
2100 TCCGGA
MseI 155 TTAA T^TAA
3089 TTAA
NspI 1113 ACATGC [GA]CATG^[CT]
2053 GCATGT
PaeR7I 67 CTCGAG C^TCGAG
1381 CTCGAG
PflMI 506 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCAGCAAATGG
PstI 1574 CTGCAG CTGCA^G
1805 CTGCAG
SapI 1613 GAAGAGC GCTCTTC(1/4)
2429 GAAGAGC
TfiI 3113 GATTC G^A[AT]TC
3183 GAATC
Tru9I 155 TTAA T^TAA
3089 TTAA
Van91I 506 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2993 CCAGCAAATGG
XhoI 67 CTCGAG C^TCGAG
1381 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BalI BanI BanII BbeI
BbsI BbvI BcgI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
StyI TaqI TseI Tsp45I TspRI
TthHB8I XhoII XmaCI XmaI