################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflIII AgeI AhdI AocI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BbrPI BclI BsaAI BsaI BsiEI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FbaI FseI FspI HindIII HpaI KpnI KspI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AflII 3088 CTTAAG C^TTAAG Alw44I 1440 GTGCAC G^TGCAC Aor51HI 1617 AGCGCT AGC^GCT ApaLI 1440 GTGCAC G^TGCAC AvrII 2423 CCTAGG C^CTAGG BamHI 2103 GGATCC G^GATCC BbvCI 656 GCTGAGG CCTCAGC(-5/-2) BciVI 2927 GTATCC GTATCC(6/5) BfrI 3088 CTTAAG C^TTAAG BlnI 2423 CCTAGG C^CTAGG BsaBI 850 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmI 1376 GAATGC GAATGC(1/-1) BspHI 1956 TCATGA T^CATGA BsrDI 1933 CATTGC GCAATG(2/0) BsrFI 1121 GCCGGC [GA]^CCGG[CT] Cfr10I 1121 GCCGGC [GA]^CCGG[CT] DraI 154 TTTAAA TTT^AAA Eco47III 1617 AGCGCT AGC^GCT EcoRV 1754 GATATC GAT^ATC HincII 2363 GTTGAC GT[CT]^[GA]AC HindII 2363 GTTGAC GT[CT]^[GA]AC MamI 850 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 1121 GCCGGC GCC^GGC NgoMIV 1121 GCCGGC G^CCGGC PshAI 1783 GACCCGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1956 TCATGA T^CATGA SacI 2578 GAGCTC GAGCT^C SfiI 2729 GGCCCCAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 63 AGGCCT AGG^CCT Tsp509I 2331 AATT ^AATT XcmI 2923 CCATGTATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccIII 1971 TCCGGA T^CCGGA 2100 TCCGGA BglI 1048 GCCCTCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2730 GCCCCAAGGGC BglII 697 AGATCT A^GATCT 828 AGATCT BseAI 1971 TCCGGA T^CCGGA 2100 TCCGGA BsmBI 255 CGTCTC CGTCTC(1/5) 346 CGTCTC BspEI 1971 TCCGGA T^CCGGA 2100 TCCGGA BssHII 1859 GCGCGC G^CGCGC 1861 GCGCGC BstXI 753 CCATCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2464 CCATGGAGATGG MroI 1971 TCCGGA T^CCGGA 2100 TCCGGA MseI 155 TTAA T^TAA 3089 TTAA NspI 1113 ACATGC [GA]CATG^[CT] 2053 GCATGT PaeR7I 67 CTCGAG C^TCGAG 1381 CTCGAG PflMI 506 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCAGCAAATGG PstI 1574 CTGCAG CTGCA^G 1805 CTGCAG SapI 1613 GAAGAGC GCTCTTC(1/4) 2429 GAAGAGC TfiI 3113 GATTC G^A[AT]TC 3183 GAATC Tru9I 155 TTAA T^TAA 3089 TTAA Van91I 506 CCACAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2993 CCAGCAAATGG XhoI 67 CTCGAG C^TCGAG 1381 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbeI BbsI BbvI BcgI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NcoI NdeII NlaIII NlaIV PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmaI SmlI StyI TaqI TseI Tsp45I TspRI TthHB8I XhoII XmaCI XmaI