##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AviII BamHI
BbrPI BglII BsaBI BseAI BsmI
BspDI BspEI BspLU11I BssHII Bst1107I
BstBI BstZ17I ClaI CpoI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FseI FspI HindIII
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I RsrII SacII ScaI SexAI
SfiI SfuI SgrAI SnaBI SphI
SspI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 2988 GACGTC GACGT^C
AcsI 3869 AAATTC [GA]^AATT[CT]
AflII 2554 CTTAAG C^TTAAG
ApoI 3869 AAATTC [GA]^AATT[CT]
Asp700I 3727 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BcgI 3540 GCAAATGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1231 TGATCA T^GATCA
BfrI 2554 CTTAAG C^TTAAG
BlpI 2788 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2788 GCTCAGC GC^T[ACGT]AGC
BsaAI 2118 TACGTG [CT]AC^GT[GA]
BsaWI 1834 ACCGGA [AT]^CCGG[AT]
BsiEI 117 CGATCG CG[GA][CT]^CG
BsiWI 1614 CGTACG C^GTACG
BsmBI 3835 GAGACG CGTCTC(1/5)
Bsp1407I 679 TGTACA T^GTACA
BspHI 2521 TCATGA T^CATGA
BsrBI 758 GAGCGG CCGCTC(-3/-3)
BsrDI 1292 GCAATG GCAATG(2/0)
BsrGI 679 TGTACA T^GTACA
BssSI 3164 CACGAG CACGAG(-5/-1)
BstEII 391 GGTGACC G^GT[ACGT]ACC
BstPI 391 GGTGACC G^GT[ACGT]ACC
CelII 2788 GCTCAGC GC^T[ACGT]AGC
DraI 3400 TTTAAA TTT^AAA
DrdI 3101 GACCACGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 391 GGTGACC G^GT[ACGT]ACC
FbaI 1231 TGATCA T^GATCA
HgaI 3837 GACGC GACGC(5/10)
HincII 3407 GTCGAC GT[CT]^[GA]AC
HindII 3407 GTCGAC GT[CT]^[GA]AC
NaeI 1977 GCCGGC GCC^GGC
NgoMIV 1977 GCCGGC G^CCGGC
NheI 1307 GCTAGC G^CTAGC
PvuI 117 CGATCG CGAT^CG
RcaI 2521 TCATGA T^CATGA
SalI 3407 GTCGAC G^TCGAC
SpeI 584 ACTAGT A^CTAGT
SspBI 679 TGTACA T^GTACA
XmnI 3727 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 3407 GTCGAC GT^[AC][GT]AC
3947 GTCTAC
ApaI 35 GGGCCC GGGCC^C
82 GGGCCC
AseI 3413 ATTAAT AT^TAAT
4190 ATTAAT
AsnI 3413 ATTAAT AT^TAAT
4190 ATTAAT
AvrII 1046 CCTAGG C^CTAGG
1372 CCTAGG
BbeI 826 GGCGCC GGCGC^C
3651 GGCGCC
BbvCI 662 CCTCAGC CCTCAGC(-5/-2)
2904 CCTCAGC
BlnI 1046 CCTAGG C^CTAGG
1372 CCTAGG
BspMI 3209 GCAGGT ACCTGC(4/8)
3943 GCAGGT
KasI 826 GGCGCC G^GCGCC
3651 GGCGCC
NarI 826 GGCGCC GG^CGCC
3651 GGCGCC
NcoI 1998 CCATGG C^CATGG
2794 CCATGG
PshAI 2313 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3563 GACATCAGTC
PshBI 3413 ATTAAT AT^TAAT
4190 ATTAAT
PvuII 361 CAGCTG CAG^CTG
385 CAGCTG
SacI 1746 GAGCTC GAGCT^C
2643 GAGCTC
SapI 2448 GCTCTTC GCTCTTC(1/4)
4070 GCTCTTC
SfoI 826 GGCGCC GGC^GCC
3651 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BciVI BcnI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmaI SmlI Sse8387I
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI