##################################
# NO CUT ENZYMES ( 48 enzymes) #
##################################
AatII AclI AflII AhdI Aor51HI
AscI AspEI AviII BbvCI BfrI
BsaBI BspDI BssHII BssSI BstBI
ClaI CpoI Eam1105I Eco47III EcoRV
EcoT22I FseI FspI MamI MfeI
MluI MunI NdeI NheI NruI
NsiI NspV PacI PaeR7I PmeI
PshAI Psp1406I PvuI RsrII SalI
ScaI SfuI SgrAI SpeI SphI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1931 GGTACC G^GTACC
AccIII 3934 TCCGGA T^CCGGA
AgeI 3959 ACCGGT A^CCGGT
AocI 1714 CCTCAGG CC^T[ACGT]AGG
ApaI 2231 GGGCCC GGGCC^C
AseI 2002 ATTAAT AT^TAAT
AsnI 2002 ATTAAT AT^TAAT
Asp700I 736 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1931 GGTACC G^GTACC
AspI 1010 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4862 CCTAGG C^CTAGG
BbeI 515 GGCGCC GGCGC^C
BbrPI 3583 CACGTG CAC^GTG
BcgI 187 GCAGATGTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4573 TGATCA T^GATCA
BglII 2034 AGATCT A^GATCT
BlnI 4862 CCTAGG C^CTAGG
BseAI 3934 TCCGGA T^CCGGA
BsiWI 2491 CGTACG C^GTACG
BsmI 3219 GAATGC GAATGC(1/-1)
Bsp1407I 1872 TGTACA T^GTACA
BspEI 3934 TCCGGA T^CCGGA
BspHI 4930 TCATGA T^CATGA
BsrGI 1872 TGTACA T^GTACA
BstEII 820 GGTCACC G^GT[ACGT]ACC
BstPI 820 GGTCACC G^GT[ACGT]ACC
Bsu36I 1714 CCTCAGG CC^T[ACGT]AGG
DraIII 1202 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4851 GACGCCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1714 CCTCAGG CC^T[ACGT]AGG
EcoNI 3502 CCTTAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 820 GGTCACC G^GT[ACGT]ACC
EcoRI 3700 GAATTC G^AATTC
FbaI 4573 TGATCA T^GATCA
HpaI 1078 GTTAAC GTT^AAC
KasI 515 GGCGCC G^GCGCC
KpnI 1931 GGTACC GGTAC^C
MroI 3934 TCCGGA T^CCGGA
NaeI 476 GCCGGC GCC^GGC
NarI 515 GGCGCC GG^CGCC
NgoMIV 476 GCCGGC G^CCGGC
PflFI 1010 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3959 ACCGGT A^CCGGT
PmaCI 3583 CACGTG CAC^GTG
PmlI 3583 CACGTG CAC^GTG
PshBI 2002 ATTAAT AT^TAAT
RcaI 4930 TCATGA T^CATGA
SacI 1604 GAGCTC GAGCT^C
SapI 1046 GCTCTTC GCTCTTC(1/4)
SbfI 1966 CCTGCAGG CCTGCA^GG
SexAI 2330 ACCAGGT A^CC[AT]GGT
SfoI 515 GGCGCC GGC^GCC
SmaI 1822 CCCGGG CCC^GGG
SnaBI 3267 TACGTA TAC^GTA
Sse8387I 1966 CCTGCAGG CCTGCA^GG
SspBI 1872 TGTACA T^GTACA
StuI 987 AGGCCT AGG^CCT
Tth111I 1010 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1822 CCCGGG C^CCGGG
XmaI 1822 CCCGGG C^CCGGG
XmnI 736 GAACAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 3428 GGATCC G^GATCC
3815 GGATCC
BciVI 395 GGATAC GTATCC(6/5)
1225 GGATAC
BglI 283 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3884 GCCCCCAGGGC
BsaWI 3934 TCCGGA [AT]^CCGG[AT]
3959 ACCGGT
BspLU11I 4434 ACATGT A^CATGT
4840 ACATGT
Bst1107I 1678 GTATAC GTA^TAC
5046 GTATAC
BstXI 485 CCATGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1095 CCAGCATTGTGG
BstZ17I 1678 GTATAC GTA^TAC
5046 GTATAC
EagI 47 CGGCCG C^GGCCG
410 CGGCCG
EclXI 47 CGGCCG C^GGCCG
410 CGGCCG
Eco52I 47 CGGCCG C^GGCCG
410 CGGCCG
HaeII 515 GGCGCC [GA]GCGC^[CT]
3340 GGCGCT
HindIII 2988 AAGCTT A^AGCTT
4813 AAGCTT
NcoI 1291 CCATGG C^CATGG
2804 CCATGG
NotI 46 GCGGCCGC GC^GGCCGC
409 GCGGCCGC
SfiI 282 GGCCCGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3883 GGCCCCCAGGGCC
SmlI 1757 CTCAAG C^T[CT][GA]AG
4026 CTCAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI ApaLI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
KspI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII