################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AatII AclI AflII AgeI AhdI AocI Aor51HI ApaI AscI Asp700I AspEI AviII AvrII BbrPI BbvCI BclI BfrI BlnI BsaAI BsaBI BsiEI BsiWI BspHI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MunI NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SapI SfiI SfuI SmaI SnaBI SpeI SwaI Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 2518 TCCGGA T^CCGGA Alw44I 1464 GTGCAC G^TGCAC ApaLI 1464 GTGCAC G^TGCAC AseI 1851 ATTAAT AT^TAAT AsnI 1851 ATTAAT AT^TAAT AspI 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2336 GGATCC G^GATCC BcgI 202 CGAGGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2683 GTATCC GTATCC(6/5) BlpI 872 GCTGAGC GC^T[ACGT]AGC Bpu1102I 872 GCTGAGC GC^T[ACGT]AGC BsaWI 2518 TCCGGA [AT]^CCGG[AT] BseAI 2518 TCCGGA T^CCGGA BsmBI 277 GAGACG CGTCTC(1/5) BsmI 456 GCATTC GAATGC(1/-1) BspDI 1203 ATCGAT AT^CGAT BspEI 2518 TCCGGA T^CCGGA BsrDI 2557 GCAATG GCAATG(2/0) BstXI 2033 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 872 GCTGAGC GC^T[ACGT]AGC ClaI 1203 ATCGAT AT^CGAT DraII 605 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 1355 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 605 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoT22I 3559 ATGCAT ATGCA^T MroI 2518 TCCGGA T^CCGGA NdeI 1633 CATATG CA^TATG NsiI 3559 ATGCAT ATGCA^T PaeR7I 2957 CTCGAG C^TCGAG PflFI 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 1851 ATTAAT AT^TAAT PvuII 3728 CAGCTG CAG^CTG SbfI 746 CCTGCAGG CCTGCA^GG ScaI 3395 AGTACT AGT^ACT SexAI 1645 ACCTGGT A^CC[AT]GGT SgrAI 105 CACCGGCG C[GA]^CCGG[CT]G SphI 2743 GCATGC GCATG^C Sse8387I 746 CCTGCAGG CCTGCA^GG SspI 3406 AATATT AAT^ATT Tth111I 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2957 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Acc65I 1230 GGTACC G^GTACC 4073 GGTACC AccI 3764 GTAGAC GT^[AC][GT]AC 3811 GTCTAC Asp718I 1230 GGTACC G^GTACC 4073 GGTACC BbsI 3133 GAAGAC GAAGAC(2/6) 1482 GTCTTC BglII 730 AGATCT A^GATCT 3196 AGATCT BpuAI 3133 GAAGAC GAAGAC(2/6) 1482 GTCTTC Bsp1407I 1615 TGTACA T^GTACA 4090 TGTACA BspLU11I 2615 ACATGT A^CATGT 4087 ACATGT BsrGI 1615 TGTACA T^GTACA 4090 TGTACA BssSI 309 CTCGTG CACGAG(-5/-1) 1914 CTCGTG BstAPI 2456 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3354 GCAGAGGCTGC BstEII 908 GGTAACC G^GT[ACGT]ACC 1607 GGTTACC BstPI 908 GGTAACC G^GT[ACGT]ACC 1607 GGTTACC DraI 3519 TTTAAA TTT^AAA 4182 TTTAAA EcoO65I 908 GGTAACC G^GT[ACGT]ACC 1607 GGTTACC EcoRI 3905 GAATTC G^AATTC 4171 GAATTC HgaI 13 GACGC GACGC(5/10) 123 GACGC HincII 2619 GTCAAC GT[CT]^[GA]AC 2871 GTCAAC HindII 2619 GTCAAC GT[CT]^[GA]AC 2871 GTCAAC KpnI 1230 GGTACC GGTAC^C 4073 GGTACC SspBI 1615 TGTACA T^GTACA 4090 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbeI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII