##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
AocI Aor51HI ApaI AscI Asp700I
AspEI AviII AvrII BbrPI BbvCI
BclI BfrI BlnI BsaAI BsaBI
BsiEI BsiWI BspHI BssHII Bst1107I
BstBI BstZ17I Bsu36I CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRV FbaI FseI FspI
HindIII HpaI KspI MamI MfeI
MluI MunI NheI NotI NruI
NspV PacI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SfiI SfuI SmaI SnaBI
SpeI SwaI Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2518 TCCGGA T^CCGGA
Alw44I 1464 GTGCAC G^TGCAC
ApaLI 1464 GTGCAC G^TGCAC
AseI 1851 ATTAAT AT^TAAT
AsnI 1851 ATTAAT AT^TAAT
AspI 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2336 GGATCC G^GATCC
BcgI 202 CGAGGATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2683 GTATCC GTATCC(6/5)
BlpI 872 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 872 GCTGAGC GC^T[ACGT]AGC
BsaWI 2518 TCCGGA [AT]^CCGG[AT]
BseAI 2518 TCCGGA T^CCGGA
BsmBI 277 GAGACG CGTCTC(1/5)
BsmI 456 GCATTC GAATGC(1/-1)
BspDI 1203 ATCGAT AT^CGAT
BspEI 2518 TCCGGA T^CCGGA
BsrDI 2557 GCAATG GCAATG(2/0)
BstXI 2033 CCAGTGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 872 GCTGAGC GC^T[ACGT]AGC
ClaI 1203 ATCGAT AT^CGAT
DraII 605 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1355 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 605 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 3559 ATGCAT ATGCA^T
MroI 2518 TCCGGA T^CCGGA
NdeI 1633 CATATG CA^TATG
NsiI 3559 ATGCAT ATGCA^T
PaeR7I 2957 CTCGAG C^TCGAG
PflFI 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 1851 ATTAAT AT^TAAT
PvuII 3728 CAGCTG CAG^CTG
SbfI 746 CCTGCAGG CCTGCA^GG
ScaI 3395 AGTACT AGT^ACT
SexAI 1645 ACCTGGT A^CC[AT]GGT
SgrAI 105 CACCGGCG C[GA]^CCGG[CT]G
SphI 2743 GCATGC GCATG^C
Sse8387I 746 CCTGCAGG CCTGCA^GG
SspI 3406 AATATT AAT^ATT
Tth111I 381 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2957 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Acc65I 1230 GGTACC G^GTACC
4073 GGTACC
AccI 3764 GTAGAC GT^[AC][GT]AC
3811 GTCTAC
Asp718I 1230 GGTACC G^GTACC
4073 GGTACC
BbsI 3133 GAAGAC GAAGAC(2/6)
1482 GTCTTC
BglII 730 AGATCT A^GATCT
3196 AGATCT
BpuAI 3133 GAAGAC GAAGAC(2/6)
1482 GTCTTC
Bsp1407I 1615 TGTACA T^GTACA
4090 TGTACA
BspLU11I 2615 ACATGT A^CATGT
4087 ACATGT
BsrGI 1615 TGTACA T^GTACA
4090 TGTACA
BssSI 309 CTCGTG CACGAG(-5/-1)
1914 CTCGTG
BstAPI 2456 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3354 GCAGAGGCTGC
BstEII 908 GGTAACC G^GT[ACGT]ACC
1607 GGTTACC
BstPI 908 GGTAACC G^GT[ACGT]ACC
1607 GGTTACC
DraI 3519 TTTAAA TTT^AAA
4182 TTTAAA
EcoO65I 908 GGTAACC G^GT[ACGT]ACC
1607 GGTTACC
EcoRI 3905 GAATTC G^AATTC
4171 GAATTC
HgaI 13 GACGC GACGC(5/10)
123 GACGC
HincII 2619 GTCAAC GT[CT]^[GA]AC
2871 GTCAAC
HindII 2619 GTCAAC GT[CT]^[GA]AC
2871 GTCAAC
KpnI 1230 GGTACC GGTAC^C
4073 GGTACC
SspBI 1615 TGTACA T^GTACA
4090 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoNI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NarI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SfoI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII