##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AhdI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BclI BfrI BlnI BsaBI
BsmBI BsmI BspDI BsrDI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I ClaI DraIII DrdI Eam1105I
EcoO65I EcoRV EcoT22I FbaI HincII
HindII HpaI KpnI MamI MluI
NdeI NheI NotI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshAI PshBI Psp1406I PvuI SalI
SfiI SfuI SgrAI SnaBI SspI
SwaI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 3120 GAATTC [GA]^AATT[CT]
Alw44I 3494 GTGCAC G^TGCAC
AlwI 2856 GGATC GGATC(4/5)
ApaLI 3494 GTGCAC G^TGCAC
ApoI 3120 GAATTC [GA]^AATT[CT]
BalI 2469 TGGCCA TGG^CCA
BbeI 2078 GGCGCC GGCGC^C
BciVI 1539 GGATAC GTATCC(6/5)
BglI 1409 GCCGCGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiWI 2142 CGTACG C^GTACG
Bsp1407I 1439 TGTACA T^GTACA
BspHI 41 TCATGA T^CATGA
BspLU11I 1281 ACATGT A^CATGT
BsrGI 1439 TGTACA T^GTACA
BssSI 619 CACGAG CACGAG(-5/-1)
CpoI 2190 CGGTCCG CG^G[AT]CCG
EcoRI 3120 GAATTC G^AATTC
FseI 2683 GGCCGGCC GGCCGG^CC
KasI 2078 GGCGCC G^GCGCC
KspI 2430 CCGCGG CCGC^GG
MfeI 2280 CAATTG C^AATTG
MluNI 2469 TGGCCA TGG^CCA
MscI 2469 TGGCCA TGG^CCA
MunI 2280 CAATTG C^AATTG
NarI 2078 GGCGCC GG^CGCC
NcoI 1967 CCATGG C^CATGG
RcaI 41 TCATGA T^CATGA
RsrII 2190 CGGTCCG CG^G[AT]CCG
SacII 2430 CCGCGG CCGC^GG
SbfI 1995 CCTGCAGG CCTGCA^GG
SexAI 2994 ACCTGGT A^CC[AT]GGT
SfoI 2078 GGCGCC GGC^GCC
SmlI 4 CTTGAG C^T[CT][GA]AG
SpeI 3580 ACTAGT A^CTAGT
SphI 1779 GCATGC GCATG^C
Sse8387I 1995 CCTGCAGG CCTGCA^GG
SspBI 1439 TGTACA T^GTACA
StuI 223 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccIII 1199 TCCGGA T^CCGGA
2193 TCCGGA
AcyI 2078 GGCGCC G[GA]^CG[CT]C
2200 GACGCC
AflIII 1281 ACATGT A^C[GA][CT]GT
1610 ACGTGT
Aor51HI 2454 AGCGCT AGC^GCT
2525 AGCGCT
BbrPI 1609 CACGTG CAC^GTG
2518 CACGTG
BglII 858 AGATCT A^GATCT
1026 AGATCT
BlpI 1296 GCTGAGC GC^T[ACGT]AGC
1374 GCTGAGC
Bpu1102I 1296 GCTGAGC GC^T[ACGT]AGC
1374 GCTGAGC
BsaAI 1609 CACGTG [CT]AC^GT[GA]
2518 CACGTG
BsaHI 2078 GGCGCC G[GA]^CG[CT]C
2200 GACGCC
BseAI 1199 TCCGGA T^CCGGA
2193 TCCGGA
BspEI 1199 TCCGGA T^CCGGA
2193 TCCGGA
BstAPI 759 GCAGAGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1882 GCAGCCAATGC
CelII 1296 GCTGAGC GC^T[ACGT]AGC
1374 GCTGAGC
DraI 3276 TTTAAA TTT^AAA
3713 TTTAAA
Eco47III 2454 AGCGCT AGC^GCT
2525 AGCGCT
EcoNI 1802 CCTCATACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3588 CCTTCCTCAGG
Hin1I 2078 GGCGCC G[GA]^CG[CT]C
2200 GACGCC
MroI 1199 TCCGGA T^CCGGA
2193 TCCGGA
MseI 3277 TTAA T^TAA
3714 TTAA
NruI 2259 TCGCGA TCG^CGA
2292 TCGCGA
NspI 1281 ACATGT [GA]CATG^[CT]
1779 GCATGC
PmaCI 1609 CACGTG CAC^GTG
2518 CACGTG
PmlI 1609 CACGTG CAC^GTG
2518 CACGTG
SacI 2564 GAGCTC GAGCT^C
2707 GAGCTC
SapI 697 GAAGAGC GCTCTTC(1/4)
657 GCTCTTC
ScaI 212 AGTACT AGT^ACT
1052 AGTACT
SmaI 3230 CCCGGG CCC^GGG
3301 CCCGGG
Tru9I 3277 TTAA T^TAA
3714 TTAA
XmaCI 3230 CCCGGG C^CCGGG
3301 CCCGGG
XmaI 3230 CCCGGG C^CCGGG
3301 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AfaI AluI AlwNI
AocI ApaI AspHI AvaI AvaII
AviII BanI BanII BbsI BbvCI
BbvI BcgI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsaWI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco52I Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII