##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AgeI Aor51HI ApaI AscI
AseI AsnI Asp718I AvaI AviII
AvrII BamHI BbeI BlnI BlpI
Bpu1102I BsiEI BsiWI BsmBI BsoBI
BspDI BspHI BssHII BssSI Bst1107I
BstEII BstPI BstZ17I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI HindIII
KasI KpnI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SalI SapI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 274 GACGTC GACGT^C
AccI 3722 GTAGAC GT^[AC][GT]AC
AccIII 1081 TCCGGA T^CCGGA
AclI 2710 AACGTT AA^CGTT
AflII 3254 CTTAAG C^TTAAG
AhdI 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 4617 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1663 CCTGAGG CC^T[ACGT]AGG
AspEI 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 4357 TGGCCA TGG^CCA
BbvCI 663 CCTCAGC CCTCAGC(-5/-2)
BfrI 3254 CTTAAG C^TTAAG
BglII 727 AGATCT A^GATCT
BseAI 1081 TCCGGA T^CCGGA
BspEI 1081 TCCGGA T^CCGGA
BstAPI 2406 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3002 TTCGAA TT^CGAA
BstXI 2160 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 727 AGATCT [GA]^GATC[CT]
Bsu36I 1663 CCTGAGG CC^T[ACGT]AGG
DraIII 1789 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1663 CCTGAGG CC^T[ACGT]AGG
EcoNI 2212 CCTCCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1431 ATGCAT ATGCA^T
HpaI 1274 GTTAAC GTT^AAC
KspI 2129 CCGCGG CCGC^GG
MflI 727 AGATCT [GA]^GATC[CT]
MluNI 4357 TGGCCA TGG^CCA
MroI 1081 TCCGGA T^CCGGA
MscI 4357 TGGCCA TGG^CCA
NsiI 1431 ATGCAT ATGCA^T
NspV 3002 TTCGAA TT^CGAA
PflMI 1224 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 2710 AACGTT AA^CGTT
SacI 1985 GAGCTC GAGCT^C
SacII 2129 CCGCGG CCGC^GG
SbfI 3914 CCTGCAGG CCTGCA^GG
SfuI 3002 TTCGAA TT^CGAA
SmlI 3254 CTTAAG C^T[CT][GA]AG
SphI 1783 GCATGC GCATG^C
Sse8387I 3914 CCTGCAGG CCTGCA^GG
Van91I 1224 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 158 CCACTGTATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 727 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspI 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACAACGTC
BbrPI 786 CACGTG CAC^GTG
2769 CACGTG
BcgI 333 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1760 CGAGGAAGGTGC
BsaBI 3562 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4680 GATTTAAATC
BsaWI 1081 TCCGGA [AT]^CCGG[AT]
2346 ACCGGA
BsgI 1991 CTGCAC GTGCAG(16/14)
4623 CTGCAC
Bsp1407I 517 TGTACA T^GTACA
3817 TGTACA
BspLU11I 1137 ACATGT A^CATGT
1820 ACATGT
BsrBI 2562 CCGCTC CCGCTC(-3/-3)
107 GAGCGG
BsrFI 2054 ACCGGC [GA]^CCGG[CT]
2177 GCCGGT
BsrGI 517 TGTACA T^GTACA
3817 TGTACA
Cfr10I 2054 ACCGGC [GA]^CCGG[CT]
2177 GCCGGT
DrdI 3923 GACAAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3991 GACAATGAAGTC
EcoRI 289 GAATTC G^AATTC
4065 GAATTC
HaeII 2479 AGCGCC [GA]GCGC^[CT]
4334 AGCGCC
HincII 277 GTCAAC GT[CT]^[GA]AC
1274 GTTAAC
HindII 277 GTCAAC GT[CT]^[GA]AC
1274 GTTAAC
MamI 3562 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4680 GATTTAAATC
PflFI 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACAACGTC
PmaCI 786 CACGTG CAC^GTG
2769 CACGTG
PmlI 786 CACGTG CAC^GTG
2769 CACGTG
ScaI 1506 AGTACT AGT^ACT
3762 AGTACT
SspBI 517 TGTACA T^GTACA
3817 TGTACA
SspI 686 AATATT AAT^ATT
1699 AATATT
StuI 2220 AGGCCT AGG^CCT
4674 AGGCCT
SwaI 3689 ATTTAAAT ATTT^AAAT
4681 ATTTAAAT
Tth111I 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3377 GACAACGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI ApaLI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BciVI BclI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI