################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## Acc65I AgeI Aor51HI ApaI AscI AseI AsnI Asp718I AvaI AviII AvrII BamHI BbeI BlnI BlpI Bpu1102I BsiEI BsiWI BsmBI BsoBI BspDI BspHI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI HindIII KasI KpnI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RcaI RsrII SalI SapI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 274 GACGTC GACGT^C AccI 3722 GTAGAC GT^[AC][GT]AC AccIII 1081 TCCGGA T^CCGGA AclI 2710 AACGTT AA^CGTT AflII 3254 CTTAAG C^TTAAG AhdI 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwNI 4617 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1663 CCTGAGG CC^T[ACGT]AGG AspEI 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 4357 TGGCCA TGG^CCA BbvCI 663 CCTCAGC CCTCAGC(-5/-2) BfrI 3254 CTTAAG C^TTAAG BglII 727 AGATCT A^GATCT BseAI 1081 TCCGGA T^CCGGA BspEI 1081 TCCGGA T^CCGGA BstAPI 2406 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3002 TTCGAA TT^CGAA BstXI 2160 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 727 AGATCT [GA]^GATC[CT] Bsu36I 1663 CCTGAGG CC^T[ACGT]AGG DraIII 1789 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 2173 GACTGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 1663 CCTGAGG CC^T[ACGT]AGG EcoNI 2212 CCTCCGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1431 ATGCAT ATGCA^T HpaI 1274 GTTAAC GTT^AAC KspI 2129 CCGCGG CCGC^GG MflI 727 AGATCT [GA]^GATC[CT] MluNI 4357 TGGCCA TGG^CCA MroI 1081 TCCGGA T^CCGGA MscI 4357 TGGCCA TGG^CCA NsiI 1431 ATGCAT ATGCA^T NspV 3002 TTCGAA TT^CGAA PflMI 1224 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 2710 AACGTT AA^CGTT SacI 1985 GAGCTC GAGCT^C SacII 2129 CCGCGG CCGC^GG SbfI 3914 CCTGCAGG CCTGCA^GG SfuI 3002 TTCGAA TT^CGAA SmlI 3254 CTTAAG C^T[CT][GA]AG SphI 1783 GCATGC GCATG^C Sse8387I 3914 CCTGCAGG CCTGCA^GG Van91I 1224 CCACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 158 CCACTGTATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 727 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AspI 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3377 GACAACGTC BbrPI 786 CACGTG CAC^GTG 2769 CACGTG BcgI 333 CGACAAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1760 CGAGGAAGGTGC BsaBI 3562 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4680 GATTTAAATC BsaWI 1081 TCCGGA [AT]^CCGG[AT] 2346 ACCGGA BsgI 1991 CTGCAC GTGCAG(16/14) 4623 CTGCAC Bsp1407I 517 TGTACA T^GTACA 3817 TGTACA BspLU11I 1137 ACATGT A^CATGT 1820 ACATGT BsrBI 2562 CCGCTC CCGCTC(-3/-3) 107 GAGCGG BsrFI 2054 ACCGGC [GA]^CCGG[CT] 2177 GCCGGT BsrGI 517 TGTACA T^GTACA 3817 TGTACA Cfr10I 2054 ACCGGC [GA]^CCGG[CT] 2177 GCCGGT DrdI 3923 GACAAATGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3991 GACAATGAAGTC EcoRI 289 GAATTC G^AATTC 4065 GAATTC HaeII 2479 AGCGCC [GA]GCGC^[CT] 4334 AGCGCC HincII 277 GTCAAC GT[CT]^[GA]AC 1274 GTTAAC HindII 277 GTCAAC GT[CT]^[GA]AC 1274 GTTAAC MamI 3562 GATGCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4680 GATTTAAATC PflFI 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3377 GACAACGTC PmaCI 786 CACGTG CAC^GTG 2769 CACGTG PmlI 786 CACGTG CAC^GTG 2769 CACGTG ScaI 1506 AGTACT AGT^ACT 3762 AGTACT SspBI 517 TGTACA T^GTACA 3817 TGTACA SspI 686 AATATT AAT^ATT 1699 AATATT StuI 2220 AGGCCT AGG^CCT 4674 AGGCCT SwaI 3689 ATTTAAAT ATTT^AAAT 4681 ATTTAAAT Tth111I 922 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3377 GACAACGTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI AvaII BanI BanII BbsI BbvI BciVI BclI BcnI BfaI BglI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI