################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AatII AccI AclI AflII AseI AsnI AspI AviII BclI BfrI BglI BglII BsaBI BsiEI BsiWI Bsp1407I BspDI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRV FbaI FseI FspI KspI MamI MluI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1640 GGTACC G^GTACC AccIII 1717 TCCGGA T^CCGGA AgeI 1859 ACCGGT A^CCGGT Alw44I 3627 GTGCAC G^TGCAC Aor51HI 831 AGCGCT AGC^GCT ApaLI 3627 GTGCAC G^TGCAC AscI 436 GGCGCGCC GG^CGCGCC Asp718I 1640 GGTACC G^GTACC AvrII 2880 CCTAGG C^CTAGG BamHI 1426 GGATCC G^GATCC BbvCI 1782 GCTGAGG CCTCAGC(-5/-2) BlnI 2880 CCTAGG C^CTAGG BlpI 2313 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2313 GCTGAGC GC^T[ACGT]AGC BseAI 1717 TCCGGA T^CCGGA BsmI 1592 GCATTC GAATGC(1/-1) BspEI 1717 TCCGGA T^CCGGA BspHI 3160 TCATGA T^CATGA BspLU11I 819 ACATGT A^CATGT BstEII 2249 GGTTACC G^GT[ACGT]ACC BstPI 2249 GGTTACC G^GT[ACGT]ACC BstXI 1072 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 2313 GCTGAGC GC^T[ACGT]AGC DraIII 426 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 831 AGCGCT AGC^GCT EcoO65I 2249 GGTTACC G^GT[ACGT]ACC EcoT22I 2646 ATGCAT ATGCA^T KpnI 1640 GGTACC GGTAC^C MroI 1717 TCCGGA T^CCGGA NcoI 220 CCATGG C^CATGG NsiI 2646 ATGCAT ATGCA^T PflMI 2491 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1859 ACCGGT A^CCGGT RcaI 3160 TCATGA T^CATGA SmaI 206 CCCGGG CCC^GGG SphI 889 GCATGC GCATG^C Van91I 2491 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 206 CCCGGG C^CCGGG XmaI 206 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 36 enzymes) # ################################### AcyI 301 GGCGCC G[GA]^CG[CT]C 360 GGCGCC AflIII 819 ACATGT A^C[GA][CT]GT 3793 ACGTGT AhdI 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3164 GACCCTGTGTC AocI 2720 CCTGAGG CC^T[ACGT]AGG 3735 CCTGAGG ApaI 396 GGGCCC GGGCC^C 2214 GGGCCC AspEI 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3164 GACCCTGTGTC BalI 901 TGGCCA TGG^CCA 2581 TGGCCA BbeI 301 GGCGCC GGCGC^C 360 GGCGCC BcgI 965 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2527 GCACGTGGCTCG BsaHI 301 GGCGCC G[GA]^CG[CT]C 360 GGCGCC BsgI 3730 CTGCAC GTGCAG(16/14) 483 GTGCAG BsrBI 455 GAGCGG CCGCTC(-3/-3) 1461 GAGCGG BssHII 227 GCGCGC G^CGCGC 437 GCGCGC Bsu36I 2720 CCTGAGG CC^T[ACGT]AGG 3735 CCTGAGG DrdI 986 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3116 GACTCTTAGGTC DsaI 220 CCATGG C^C[GA][CT]GG 3511 CCACGG EaeI 901 TGGCCA [CT]^GGCC[GA] 2581 TGGCCA Eam1105I 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3164 GACCCTGTGTC Eco81I 2720 CCTGAGG CC^T[ACGT]AGG 3735 CCTGAGG EcoNI 2199 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2515 CCTTCTCAAGG EcoRI 1634 GAATTC G^AATTC 2172 GAATTC HgaI 3239 GACGC GACGC(5/10) 3054 GCGTC Hin1I 301 GGCGCC G[GA]^CG[CT]C 360 GGCGCC HindIII 1378 AAGCTT A^AGCTT 1976 AAGCTT HpaI 1863 GTTAAC GTT^AAC 3299 GTTAAC KasI 301 GGCGCC G^GCGCC 360 GGCGCC MfeI 3129 CAATTG C^AATTG 3759 CAATTG MluNI 901 TGGCCA TGG^CCA 2581 TGGCCA MscI 901 TGGCCA TGG^CCA 2581 TGGCCA MunI 3129 CAATTG C^AATTG 3759 CAATTG NarI 301 GGCGCC GG^CGCC 360 GGCGCC NdeI 765 CATATG CA^TATG 2765 CATATG PpuMI 1261 AGGACCT [GA]G^G[AT]CC[CT] 3044 GGGTCCT SfoI 301 GGCGCC GGC^GCC 360 GGCGCC SpeI 1965 ACTAGT A^CTAGT 3281 ACTAGT XcmI 178 CCAGCTCTAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2104 CCATGCCAGTGTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbrPI BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI