##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AccI AclI AflII AseI
AsnI AspI AviII BclI BfrI
BglI BglII BsaBI BsiEI BsiWI
Bsp1407I BspDI BsrDI BsrGI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI EagI EclXI Eco52I EcoRV
FbaI FseI FspI KspI MamI
MluI NaeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI Sse8387I SspBI SspI StuI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1640 GGTACC G^GTACC
AccIII 1717 TCCGGA T^CCGGA
AgeI 1859 ACCGGT A^CCGGT
Alw44I 3627 GTGCAC G^TGCAC
Aor51HI 831 AGCGCT AGC^GCT
ApaLI 3627 GTGCAC G^TGCAC
AscI 436 GGCGCGCC GG^CGCGCC
Asp718I 1640 GGTACC G^GTACC
AvrII 2880 CCTAGG C^CTAGG
BamHI 1426 GGATCC G^GATCC
BbvCI 1782 GCTGAGG CCTCAGC(-5/-2)
BlnI 2880 CCTAGG C^CTAGG
BlpI 2313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2313 GCTGAGC GC^T[ACGT]AGC
BseAI 1717 TCCGGA T^CCGGA
BsmI 1592 GCATTC GAATGC(1/-1)
BspEI 1717 TCCGGA T^CCGGA
BspHI 3160 TCATGA T^CATGA
BspLU11I 819 ACATGT A^CATGT
BstEII 2249 GGTTACC G^GT[ACGT]ACC
BstPI 2249 GGTTACC G^GT[ACGT]ACC
BstXI 1072 CCATCGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2313 GCTGAGC GC^T[ACGT]AGC
DraIII 426 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 831 AGCGCT AGC^GCT
EcoO65I 2249 GGTTACC G^GT[ACGT]ACC
EcoT22I 2646 ATGCAT ATGCA^T
KpnI 1640 GGTACC GGTAC^C
MroI 1717 TCCGGA T^CCGGA
NcoI 220 CCATGG C^CATGG
NsiI 2646 ATGCAT ATGCA^T
PflMI 2491 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1859 ACCGGT A^CCGGT
RcaI 3160 TCATGA T^CATGA
SmaI 206 CCCGGG CCC^GGG
SphI 889 GCATGC GCATG^C
Van91I 2491 CCAGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 206 CCCGGG C^CCGGG
XmaI 206 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcyI 301 GGCGCC G[GA]^CG[CT]C
360 GGCGCC
AflIII 819 ACATGT A^C[GA][CT]GT
3793 ACGTGT
AhdI 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3164 GACCCTGTGTC
AocI 2720 CCTGAGG CC^T[ACGT]AGG
3735 CCTGAGG
ApaI 396 GGGCCC GGGCC^C
2214 GGGCCC
AspEI 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3164 GACCCTGTGTC
BalI 901 TGGCCA TGG^CCA
2581 TGGCCA
BbeI 301 GGCGCC GGCGC^C
360 GGCGCC
BcgI 965 CGACACAGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2527 GCACGTGGCTCG
BsaHI 301 GGCGCC G[GA]^CG[CT]C
360 GGCGCC
BsgI 3730 CTGCAC GTGCAG(16/14)
483 GTGCAG
BsrBI 455 GAGCGG CCGCTC(-3/-3)
1461 GAGCGG
BssHII 227 GCGCGC G^CGCGC
437 GCGCGC
Bsu36I 2720 CCTGAGG CC^T[ACGT]AGG
3735 CCTGAGG
DrdI 986 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3116 GACTCTTAGGTC
DsaI 220 CCATGG C^C[GA][CT]GG
3511 CCACGG
EaeI 901 TGGCCA [CT]^GGCC[GA]
2581 TGGCCA
Eam1105I 3080 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3164 GACCCTGTGTC
Eco81I 2720 CCTGAGG CC^T[ACGT]AGG
3735 CCTGAGG
EcoNI 2199 CCTGAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2515 CCTTCTCAAGG
EcoRI 1634 GAATTC G^AATTC
2172 GAATTC
HgaI 3239 GACGC GACGC(5/10)
3054 GCGTC
Hin1I 301 GGCGCC G[GA]^CG[CT]C
360 GGCGCC
HindIII 1378 AAGCTT A^AGCTT
1976 AAGCTT
HpaI 1863 GTTAAC GTT^AAC
3299 GTTAAC
KasI 301 GGCGCC G^GCGCC
360 GGCGCC
MfeI 3129 CAATTG C^AATTG
3759 CAATTG
MluNI 901 TGGCCA TGG^CCA
2581 TGGCCA
MscI 901 TGGCCA TGG^CCA
2581 TGGCCA
MunI 3129 CAATTG C^AATTG
3759 CAATTG
NarI 301 GGCGCC GG^CGCC
360 GGCGCC
NdeI 765 CATATG CA^TATG
2765 CATATG
PpuMI 1261 AGGACCT [GA]G^G[AT]CC[CT]
3044 GGGTCCT
SfoI 301 GGCGCC GGC^GCC
360 GGCGCC
SpeI 1965 ACTAGT A^CTAGT
3281 ACTAGT
XcmI 178 CCAGCTCTAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2104 CCATGCCAGTGTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BanI BanII BbrPI
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI