################################## # NO CUT ENZYMES ( 103 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI AocI ApaI AscI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanI BbeI BcgI BciVI BfrI BglII BlnI BsaBI BsaHI BseAI BsiEI BsiWI BsoBI BspDI BspEI BspHI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I ClaI CpoI DraIII DsaI EaeI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MluNI MroI MscI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI Sse8387I SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 4316 GTCTAC GT^[AC][GT]AC AccII 4030 CGCG CG^CG AlwNI 1725 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 212 AGCGCT AGC^GCT AseI 3116 ATTAAT AT^TAAT AsnI 3116 ATTAAT AT^TAAT BbrPI 3002 CACGTG CAC^GTG BclI 809 TGATCA T^GATCA BglI 1553 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 91 ACCGGA [AT]^CCGG[AT] BsmBI 3058 GAGACG CGTCTC(1/5) BspMI 3814 ACCTGC ACCTGC(4/8) BsrBI 426 GAGCGG CCGCTC(-3/-3) BsrFI 161 ACCGGC [GA]^CCGG[CT] BstEII 87 GGTAACC G^GT[ACGT]ACC BstPI 87 GGTAACC G^GT[ACGT]ACC BstUI 4030 CGCG CG^CG CfoI 213 GCGC GCG^C Cfr10I 161 ACCGGC [GA]^CCGG[CT] DrdI 4307 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 212 AGCGCT AGC^GCT EcoNI 3927 CCTATAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 87 GGTAACC G^GT[ACGT]ACC FauI 459 GCGGG CCCGC(4/6) FbaI 809 TGATCA T^GATCA HaeII 212 AGCGCT [GA]GCGC^[CT] HgaI 950 GACGC GACGC(5/10) HhaI 213 GCGC GCG^C HinP1I 213 GCGC G^CGC HincII 1900 GTTGAC GT[CT]^[GA]AC HindII 1900 GTTGAC GT[CT]^[GA]AC MfeI 2752 CAATTG C^AATTG MunI 2752 CAATTG C^AATTG MvnI 4030 CGCG CG^CG NheI 3253 GCTAGC G^CTAGC PmaCI 3002 CACGTG CAC^GTG PmlI 3002 CACGTG CAC^GTG PshBI 3116 ATTAAT AT^TAAT SapI 1041 GAAGAGC GCTCTTC(1/4) SnaBI 2139 TACGTA TAC^GTA StuI 3924 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Alw44I 935 GTGCAC G^TGCAC 2999 GTGCAC ApaLI 935 GTGCAC G^TGCAC 2999 GTGCAC BamHI 582 GGATCC G^GATCC 719 GGATCC BbvCI 215 GCTGAGG CCTCAGC(-5/-2) 1088 GCTGAGG BcnI 662 CCGGG CC^[GC]GG 820 CCCGG BsaAI 2139 TACGTA [CT]AC^GT[GA] 3002 CACGTG BsrDI 3140 CATTGC GCAATG(2/0) 4187 GCAATG BstXI 232 CCAGGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1736 CCAGCAGGATGG EcoT14I 190 CCTTGG C^C[AT][AT]GG 4124 CCAAGG NciI 662 CCGGG CC^[GC]GG 820 CCCGG PflMI 1724 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3587 CCAGGATATGG PvuII 836 CAGCTG CAG^CTG 1363 CAGCTG SacI 120 GAGCTC GAGCT^C 1116 GAGCTC SpeI 2320 ACTAGT A^CTAGT 3753 ACTAGT StyI 190 CCTTGG C^C[AT][AT]GG 4124 CCAAGG TaqI 1083 TCGA T^CGA 1283 TCGA TthHB8I 1083 TCGA T^CGA 1283 TCGA Van91I 1724 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3587 CCAGGATATGG ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI ApoI AspHI AvaII BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BspLU11I BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT22I Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII