##################################
# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AocI ApaI
AscI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BalI BanI
BbeI BcgI BciVI BfrI BglII
BlnI BsaBI BsaHI BseAI BsiEI
BsiWI BsoBI BspDI BspEI BspHI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I Bsu36I ClaI CpoI DraIII
DsaI EaeI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV FseI
FspI Hin1I HpaI KasI KpnI
KspI MamI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI Sse8387I
SwaI Tth111I XbaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 4316 GTCTAC GT^[AC][GT]AC
AccII 4030 CGCG CG^CG
AlwNI 1725 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 212 AGCGCT AGC^GCT
AseI 3116 ATTAAT AT^TAAT
AsnI 3116 ATTAAT AT^TAAT
BbrPI 3002 CACGTG CAC^GTG
BclI 809 TGATCA T^GATCA
BglI 1553 GCCAGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 91 ACCGGA [AT]^CCGG[AT]
BsmBI 3058 GAGACG CGTCTC(1/5)
BspMI 3814 ACCTGC ACCTGC(4/8)
BsrBI 426 GAGCGG CCGCTC(-3/-3)
BsrFI 161 ACCGGC [GA]^CCGG[CT]
BstEII 87 GGTAACC G^GT[ACGT]ACC
BstPI 87 GGTAACC G^GT[ACGT]ACC
BstUI 4030 CGCG CG^CG
CfoI 213 GCGC GCG^C
Cfr10I 161 ACCGGC [GA]^CCGG[CT]
DrdI 4307 GACAACTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 212 AGCGCT AGC^GCT
EcoNI 3927 CCTATAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 87 GGTAACC G^GT[ACGT]ACC
FauI 459 GCGGG CCCGC(4/6)
FbaI 809 TGATCA T^GATCA
HaeII 212 AGCGCT [GA]GCGC^[CT]
HgaI 950 GACGC GACGC(5/10)
HhaI 213 GCGC GCG^C
HinP1I 213 GCGC G^CGC
HincII 1900 GTTGAC GT[CT]^[GA]AC
HindII 1900 GTTGAC GT[CT]^[GA]AC
MfeI 2752 CAATTG C^AATTG
MunI 2752 CAATTG C^AATTG
MvnI 4030 CGCG CG^CG
NheI 3253 GCTAGC G^CTAGC
PmaCI 3002 CACGTG CAC^GTG
PmlI 3002 CACGTG CAC^GTG
PshBI 3116 ATTAAT AT^TAAT
SapI 1041 GAAGAGC GCTCTTC(1/4)
SnaBI 2139 TACGTA TAC^GTA
StuI 3924 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 935 GTGCAC G^TGCAC
2999 GTGCAC
ApaLI 935 GTGCAC G^TGCAC
2999 GTGCAC
BamHI 582 GGATCC G^GATCC
719 GGATCC
BbvCI 215 GCTGAGG CCTCAGC(-5/-2)
1088 GCTGAGG
BcnI 662 CCGGG CC^[GC]GG
820 CCCGG
BsaAI 2139 TACGTA [CT]AC^GT[GA]
3002 CACGTG
BsrDI 3140 CATTGC GCAATG(2/0)
4187 GCAATG
BstXI 232 CCAGGACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1736 CCAGCAGGATGG
EcoT14I 190 CCTTGG C^C[AT][AT]GG
4124 CCAAGG
NciI 662 CCGGG CC^[GC]GG
820 CCCGG
PflMI 1724 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3587 CCAGGATATGG
PvuII 836 CAGCTG CAG^CTG
1363 CAGCTG
SacI 120 GAGCTC GAGCT^C
1116 GAGCTC
SpeI 2320 ACTAGT A^CTAGT
3753 ACTAGT
StyI 190 CCTTGG C^C[AT][AT]GG
4124 CCAAGG
TaqI 1083 TCGA T^CGA
1283 TCGA
TthHB8I 1083 TCGA T^CGA
1283 TCGA
Van91I 1724 CCAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3587 CCAGGATATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspLU11I BsrGI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII Cfr13I
DdeI DpnII DraI DraII EarI
EcoO109I EcoRII EcoT22I Fnu4HI FokI
HaeIII HapII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SphI SspBI SspI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII