################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AccIII AclI AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AvrII BbrPI BciVI BclI BfrI BglII BlnI BsaAI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BstBI BstEII BstPI Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI FbaI FseI HindIII HpaI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI ScaI SexAI SfiI SfuI SnaBI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 2998 GGTACC G^GTACC AcsI 4260 AAATTT [GA]^AATT[CT] Aor51HI 3232 AGCGCT AGC^GCT ApoI 4260 AAATTT [GA]^AATT[CT] Asp700I 48 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2998 GGTACC G^GTACC AspI 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 697 GGCGCC GGCGC^C BbvCI 1197 GCTGAGG CCTCAGC(-5/-2) BcgI 884 CGAGGCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 1830 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1471 ACCGGA [AT]^CCGG[AT] BspLU11I 2789 ACATGT A^CATGT BssHII 204 GCGCGC G^CGCGC BssSI 3697 CACGAG CACGAG(-5/-1) Bst1107I 3889 GTATAC GTA^TAC BstZ17I 3889 GTATAC GTA^TAC DraIII 3102 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3232 AGCGCT AGC^GCT EcoRV 4059 GATATC GAT^ATC HgaI 727 GCGTC GACGC(5/10) HincII 2095 GTCAAC GT[CT]^[GA]AC HindII 2095 GTCAAC GT[CT]^[GA]AC KasI 697 GGCGCC G^GCGCC KpnI 2998 GGTACC GGTAC^C KspI 170 CCGCGG CCGC^GG MamI 1830 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MslI 279 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 697 GGCGCC GG^CGCC PaeR7I 2617 CTCGAG C^TCGAG PflFI 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 3529 GAGCTC GAGCT^C SacII 170 CCGCGG CCGC^GG SfoI 697 GGCGCC GGC^GCC SgrAI 3409 CACCGGCG C[GA]^CCGG[CT]G SpeI 3755 ACTAGT A^CTAGT Tth111I 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 2617 CTCGAG C^TCGAG XmnI 48 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AatII 3238 GACGTC GACGT^C 3668 GACGTC AccI 3889 GTATAC GT^[AC][GT]AC 4109 GTAGAC AflIII 2789 ACATGT A^C[GA][CT]GT 3577 ACGTGT ApaI 2486 GGGCCC GGGCC^C 3068 GGGCCC AviII 3174 TGCGCA TGC^GCA 4317 TGCGCA BamHI 334 GGATCC G^GATCC 1953 GGATCC BsmBI 728 CGTCTC CGTCTC(1/5) 3666 GAGACG BsrBI 78 CCGCTC CCGCTC(-3/-3) 1204 GAGCGG BsrDI 1763 CATTGC GCAATG(2/0) 1114 GCAATG DraI 4115 TTTAAA TTT^AAA 4255 TTTAAA FspI 3174 TGCGCA TGC^GCA 4317 TGCGCA NcoI 248 CCATGG C^CATGG 278 CCATGG NdeI 2706 CATATG CA^TATG 4129 CATATG NheI 2724 GCTAGC G^CTAGC 3217 GCTAGC PshAI 31 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4127 GACATATGTC SphI 200 GCATGC GCATG^C 4217 GCATGC TaqI 1035 TCGA T^CGA 2618 TCGA TthHB8I 1035 TCGA T^CGA 2618 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 126 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII XmaCI XmaI