##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI ApaLI AscI AseI
AsnI AspEI AvrII BbrPI BciVI
BclI BfrI BglII BlnI BsaAI
BseAI BsiEI BsiWI BsmI BspDI
BspEI BspHI BstBI BstEII BstPI
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI FbaI FseI HindIII HpaI
MfeI MluI MroI MunI NaeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI ScaI SexAI SfiI SfuI
SnaBI Sse8387I SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2998 GGTACC G^GTACC
AcsI 4260 AAATTT [GA]^AATT[CT]
Aor51HI 3232 AGCGCT AGC^GCT
ApoI 4260 AAATTT [GA]^AATT[CT]
Asp700I 48 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2998 GGTACC G^GTACC
AspI 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 697 GGCGCC GGCGC^C
BbvCI 1197 GCTGAGG CCTCAGC(-5/-2)
BcgI 884 CGAGGCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1830 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1471 ACCGGA [AT]^CCGG[AT]
BspLU11I 2789 ACATGT A^CATGT
BssHII 204 GCGCGC G^CGCGC
BssSI 3697 CACGAG CACGAG(-5/-1)
Bst1107I 3889 GTATAC GTA^TAC
BstZ17I 3889 GTATAC GTA^TAC
DraIII 3102 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3232 AGCGCT AGC^GCT
EcoRV 4059 GATATC GAT^ATC
HgaI 727 GCGTC GACGC(5/10)
HincII 2095 GTCAAC GT[CT]^[GA]AC
HindII 2095 GTCAAC GT[CT]^[GA]AC
KasI 697 GGCGCC G^GCGCC
KpnI 2998 GGTACC GGTAC^C
KspI 170 CCGCGG CCGC^GG
MamI 1830 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 279 CATGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 697 GGCGCC GG^CGCC
PaeR7I 2617 CTCGAG C^TCGAG
PflFI 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 3529 GAGCTC GAGCT^C
SacII 170 CCGCGG CCGC^GG
SfoI 697 GGCGCC GGC^GCC
SgrAI 3409 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3755 ACTAGT A^CTAGT
Tth111I 3386 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2617 CTCGAG C^TCGAG
XmnI 48 GAAGTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AatII 3238 GACGTC GACGT^C
3668 GACGTC
AccI 3889 GTATAC GT^[AC][GT]AC
4109 GTAGAC
AflIII 2789 ACATGT A^C[GA][CT]GT
3577 ACGTGT
ApaI 2486 GGGCCC GGGCC^C
3068 GGGCCC
AviII 3174 TGCGCA TGC^GCA
4317 TGCGCA
BamHI 334 GGATCC G^GATCC
1953 GGATCC
BsmBI 728 CGTCTC CGTCTC(1/5)
3666 GAGACG
BsrBI 78 CCGCTC CCGCTC(-3/-3)
1204 GAGCGG
BsrDI 1763 CATTGC GCAATG(2/0)
1114 GCAATG
DraI 4115 TTTAAA TTT^AAA
4255 TTTAAA
FspI 3174 TGCGCA TGC^GCA
4317 TGCGCA
NcoI 248 CCATGG C^CATGG
278 CCATGG
NdeI 2706 CATATG CA^TATG
4129 CATATG
NheI 2724 GCTAGC G^CTAGC
3217 GCTAGC
PshAI 31 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4127 GACATATGTC
SphI 200 GCATGC GCATG^C
4217 GCATGC
TaqI 1035 TCGA T^CGA
2618 TCGA
TthHB8I 1035 TCGA T^CGA
2618 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrFI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PpuMI PstI PvuII RsaI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StuI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII XmaCI
XmaI