##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspEI BamHI BbeI BbrPI
BcgI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
BstBI ClaI CpoI DraI Eam1105I
Eco47III EcoRI EcoRV HpaI KasI
KspI MfeI MluI MroI MunI
NarI NdeI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII ScaI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 4099 GGTACC G^GTACC
AcsI 3300 AAATTT [GA]^AATT[CT]
AcyI 103 GACGCC G[GA]^CG[CT]C
AflII 3092 CTTAAG C^TTAAG
AocI 1308 CCTCAGG CC^T[ACGT]AGG
ApoI 3300 AAATTT [GA]^AATT[CT]
Asp718I 4099 GGTACC G^GTACC
AspI 220 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 316 TGCGCA TGC^GCA
AvrII 3775 CCTAGG C^CTAGG
BclI 3910 TGATCA T^GATCA
BfrI 3092 CTTAAG C^TTAAG
BglI 1549 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3503 AGATCT A^GATCT
BlnI 3775 CCTAGG C^CTAGG
BsaAI 2562 CACGTA [CT]AC^GT[GA]
BsaBI 852 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 103 GACGCC G[GA]^CG[CT]C
BsaI 1517 GAGACC GGTCTC(1/5)
BsiEI 135 CGGCCG CG[GA][CT]^CG
BsrDI 2099 GCAATG GCAATG(2/0)
BsrFI 137 GCCGGC [GA]^CCGG[CT]
BssSI 364 CTCGTG CACGAG(-5/-1)
Bst1107I 4207 GTATAC GTA^TAC
BstEII 465 GGTGACC G^GT[ACGT]ACC
BstPI 465 GGTGACC G^GT[ACGT]ACC
BstZ17I 4207 GTATAC GTA^TAC
Bsu36I 1308 CCTCAGG CC^T[ACGT]AGG
Cfr10I 137 GCCGGC [GA]^CCGG[CT]
DrdI 1723 GACAGCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 135 CGGCCG C^GGCCG
EarI 1807 GAAGAG CTCTTC(1/4)
EclXI 135 CGGCCG C^GGCCG
Eco52I 135 CGGCCG C^GGCCG
Eco81I 1308 CCTCAGG CC^T[ACGT]AGG
EcoO65I 465 GGTGACC G^GT[ACGT]ACC
EcoT22I 747 ATGCAT ATGCA^T
FbaI 3910 TGATCA T^GATCA
FseI 136 GGCCGGCC GGCCGG^CC
FspI 316 TGCGCA TGC^GCA
Hin1I 103 GACGCC G[GA]^CG[CT]C
HindIII 2509 AAGCTT A^AGCTT
KpnI 4099 GGTACC GGTAC^C
Ksp632I 1807 GAAGAG CTCTTC(1/4)
MaeII 2563 ACGT A^CGT
MamI 852 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 137 GCCGGC GCC^GGC
NgoMIV 137 GCCGGC G^CCGGC
NsiI 747 ATGCAT ATGCA^T
PflFI 220 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2333 GACGGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 3468 GAGCTC GAGCT^C
SalI 1720 GTCGAC G^TCGAC
SapI 1807 GAAGAGC GCTCTTC(1/4)
SfiI 1548 GGCCATGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 731 CCCGGG CCC^GGG
Tsp509I 3301 AATT ^AATT
Tth111I 220 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 731 CCCGGG C^CCGGG
XmaI 731 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 1717 CGCG CG^CG
2752 CGCG
AflIII 1275 ACATGT A^C[GA][CT]GT
1480 ACATGT
ApaI 1283 GGGCCC GGGCC^C
1303 GGGCCC
Asp700I 2450 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2507 GAAAGCTTTC
BciVI 3148 GGATAC GTATCC(6/5)
2147 GTATCC
BsmBI 1264 CGTCTC CGTCTC(1/5)
2402 CGTCTC
BsmI 35 GCATTC GAATGC(1/-1)
2311 GCATTC
BspLU11I 1275 ACATGT A^CATGT
1480 ACATGT
BstUI 1717 CGCG CG^CG
2752 CGCG
DraIII 3228 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
3332 CACCTAGTG
HaeII 773 GGCGCT [GA]GCGC^[CT]
2787 GGCGCT
HincII 1720 GTCGAC GT[CT]^[GA]AC
2322 GTTGAC
HindII 1720 GTCGAC GT[CT]^[GA]AC
2322 GTTGAC
MvnI 1717 CGCG CG^CG
2752 CGCG
NcoI 1550 CCATGG C^CATGG
2613 CCATGG
SbfI 335 CCTGCAGG CCTGCA^GG
1749 CCTGCAGG
Sse8387I 335 CCTGCAGG CCTGCA^GG
1749 CCTGCAGG
XmnI 2450 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2507 GAAAGCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
TspRI TthHB8I Van91I XcmI XhoII