##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
BamHI BbrPI BcgI BciVI BglI
BsaAI BsaI BseAI BsiEI BsiWI
BsoBI Bsp1407I BspDI BspEI BsrFI
BsrGI BssHII BstAPI BstBI BstEII
BstPI BstXI Cfr10I ClaI CpoI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FseI FspI HpaI
KpnI MluI MroI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SalI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SspBI StuI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 450 CGCG CG^CG
AflII 3114 CTTAAG C^TTAAG
AocI 2700 CCTGAGG CC^T[ACGT]AGG
ApaI 395 GGGCCC GGGCC^C
AseI 3885 ATTAAT AT^TAAT
AsnI 3885 ATTAAT AT^TAAT
AvrII 3875 CCTAGG C^CTAGG
BbeI 2888 GGCGCC GGCGC^C
BbvCI 958 GCTGAGG CCTCAGC(-5/-2)
BclI 3314 TGATCA T^GATCA
BfrI 3114 CTTAAG C^TTAAG
BglII 3081 AGATCT A^GATCT
BlnI 3875 CCTAGG C^CTAGG
BsaBI 3905 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 22 TCCGGT [AT]^CCGG[AT]
BsmI 579 GCATTC GAATGC(1/-1)
BspLU11I 717 ACATGT A^CATGT
BssSI 2875 CACGAG CACGAG(-5/-1)
Bst1107I 267 GTATAC GTA^TAC
BstUI 450 CGCG CG^CG
BstZ17I 267 GTATAC GTA^TAC
Bsu36I 2700 CCTGAGG CC^T[ACGT]AGG
DraI 3796 TTTAAA TTT^AAA
DraIII 2199 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3003 GACGCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2700 CCTGAGG CC^T[ACGT]AGG
EcoRV 775 GATATC GAT^ATC
EcoT22I 3662 ATGCAT ATGCA^T
FbaI 3314 TGATCA T^GATCA
HaeII 2888 GGCGCC [GA]GCGC^[CT]
HincII 3949 GTCAAC GT[CT]^[GA]AC
HindII 3949 GTCAAC GT[CT]^[GA]AC
KasI 2888 GGCGCC G^GCGCC
KspI 449 CCGCGG CCGC^GG
MamI 3905 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2849 CAATTG C^AATTG
MunI 2849 CAATTG C^AATTG
MvnI 450 CGCG CG^CG
NarI 2888 GGCGCC GG^CGCC
NcoI 3849 CCATGG C^CATGG
NsiI 3662 ATGCAT ATGCA^T
PacI 3882 TTAATTAA TTAAT^TAA
PshBI 3885 ATTAAT AT^TAAT
SacII 449 CCGCGG CCGC^GG
SbfI 2544 CCTGCAGG CCTGCA^GG
SfoI 2888 GGCGCC GGC^GCC
SpeI 3391 ACTAGT A^CTAGT
Sse8387I 2544 CCTGCAGG CCTGCA^GG
SspI 3789 AATATT AAT^ATT
XbaI 505 TCTAGA T^CTAGA
XcmI 201 CCAGGAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 130 GTCTAC GT^[AC][GT]AC
267 GTATAC
AcyI 2531 GACGCC G[GA]^CG[CT]C
2888 GGCGCC
AflIII 717 ACATGT A^C[GA][CT]GT
2513 ACGTGT
Asp700I 1162 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2706 GAACCAGTTC
BalI 2264 TGGCCA TGG^CCA
2633 TGGCCA
BanI 2888 GGCGCC G^G[CT][GA]CC
3340 GGCACC
BcnI 361 CCCGG CC^[GC]GG
2750 CCGGG
BsaHI 2531 GACGCC G[GA]^CG[CT]C
2888 GGCGCC
BsmBI 1423 GAGACG CGTCTC(1/5)
2290 GAGACG
BspHI 768 TCATGA T^CATGA
2894 TCATGA
BspMI 1716 GCAGGT ACCTGC(4/8)
2742 GCAGGT
DsaI 449 CCGCGG C^C[GA][CT]GG
3849 CCATGG
Hin1I 2531 GACGCC G[GA]^CG[CT]C
2888 GGCGCC
HindIII 2062 AAGCTT A^AGCTT
3129 AAGCTT
MluNI 2264 TGGCCA TGG^CCA
2633 TGGCCA
MscI 2264 TGGCCA TGG^CCA
2633 TGGCCA
NciI 361 CCCGG CC^[GC]GG
2750 CCGGG
PpuMI 1226 AGGTCCT [GA]G^G[AT]CC[CT]
2183 AGGACCT
RcaI 768 TCATGA T^CATGA
2894 TCATGA
SacI 1660 GAGCTC GAGCT^C
3468 GAGCTC
SapI 550 GAAGAGC GCTCTTC(1/4)
1162 GAAGAGC
SphI 877 GCATGC GCATG^C
2828 GCATGC
XmnI 1162 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2706 GAACCAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII