################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI AspI AvaI AviII BamHI BbrPI BcgI BciVI BglI BsaAI BsaI BseAI BsiEI BsiWI BsoBI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII BstAPI BstBI BstEII BstPI BstXI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HpaI KpnI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SspBI StuI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 450 CGCG CG^CG AflII 3114 CTTAAG C^TTAAG AocI 2700 CCTGAGG CC^T[ACGT]AGG ApaI 395 GGGCCC GGGCC^C AseI 3885 ATTAAT AT^TAAT AsnI 3885 ATTAAT AT^TAAT AvrII 3875 CCTAGG C^CTAGG BbeI 2888 GGCGCC GGCGC^C BbvCI 958 GCTGAGG CCTCAGC(-5/-2) BclI 3314 TGATCA T^GATCA BfrI 3114 CTTAAG C^TTAAG BglII 3081 AGATCT A^GATCT BlnI 3875 CCTAGG C^CTAGG BsaBI 3905 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 22 TCCGGT [AT]^CCGG[AT] BsmI 579 GCATTC GAATGC(1/-1) BspLU11I 717 ACATGT A^CATGT BssSI 2875 CACGAG CACGAG(-5/-1) Bst1107I 267 GTATAC GTA^TAC BstUI 450 CGCG CG^CG BstZ17I 267 GTATAC GTA^TAC Bsu36I 2700 CCTGAGG CC^T[ACGT]AGG DraI 3796 TTTAAA TTT^AAA DraIII 2199 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 3003 GACGCTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2700 CCTGAGG CC^T[ACGT]AGG EcoRV 775 GATATC GAT^ATC EcoT22I 3662 ATGCAT ATGCA^T FbaI 3314 TGATCA T^GATCA HaeII 2888 GGCGCC [GA]GCGC^[CT] HincII 3949 GTCAAC GT[CT]^[GA]AC HindII 3949 GTCAAC GT[CT]^[GA]AC KasI 2888 GGCGCC G^GCGCC KspI 449 CCGCGG CCGC^GG MamI 3905 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2849 CAATTG C^AATTG MunI 2849 CAATTG C^AATTG MvnI 450 CGCG CG^CG NarI 2888 GGCGCC GG^CGCC NcoI 3849 CCATGG C^CATGG NsiI 3662 ATGCAT ATGCA^T PacI 3882 TTAATTAA TTAAT^TAA PshBI 3885 ATTAAT AT^TAAT SacII 449 CCGCGG CCGC^GG SbfI 2544 CCTGCAGG CCTGCA^GG SfoI 2888 GGCGCC GGC^GCC SpeI 3391 ACTAGT A^CTAGT Sse8387I 2544 CCTGCAGG CCTGCA^GG SspI 3789 AATATT AAT^ATT XbaI 505 TCTAGA T^CTAGA XcmI 201 CCAGGAAGAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 130 GTCTAC GT^[AC][GT]AC 267 GTATAC AcyI 2531 GACGCC G[GA]^CG[CT]C 2888 GGCGCC AflIII 717 ACATGT A^C[GA][CT]GT 2513 ACGTGT Asp700I 1162 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2706 GAACCAGTTC BalI 2264 TGGCCA TGG^CCA 2633 TGGCCA BanI 2888 GGCGCC G^G[CT][GA]CC 3340 GGCACC BcnI 361 CCCGG CC^[GC]GG 2750 CCGGG BsaHI 2531 GACGCC G[GA]^CG[CT]C 2888 GGCGCC BsmBI 1423 GAGACG CGTCTC(1/5) 2290 GAGACG BspHI 768 TCATGA T^CATGA 2894 TCATGA BspMI 1716 GCAGGT ACCTGC(4/8) 2742 GCAGGT DsaI 449 CCGCGG C^C[GA][CT]GG 3849 CCATGG Hin1I 2531 GACGCC G[GA]^CG[CT]C 2888 GGCGCC HindIII 2062 AAGCTT A^AGCTT 3129 AAGCTT MluNI 2264 TGGCCA TGG^CCA 2633 TGGCCA MscI 2264 TGGCCA TGG^CCA 2633 TGGCCA NciI 361 CCCGG CC^[GC]GG 2750 CCGGG PpuMI 1226 AGGTCCT [GA]G^G[AT]CC[CT] 2183 AGGACCT RcaI 768 TCATGA T^CATGA 2894 TCATGA SacI 1660 GAGCTC GAGCT^C 3468 GAGCTC SapI 550 GAAGAGC GCTCTTC(1/4) 1162 GAAGAGC SphI 877 GCATGC GCATG^C 2828 GCATGC XmnI 1162 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2706 GAACCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbsI BbvI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII