##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
Asp700I Asp718I BamHI BbeI BbvCI
BcgI BsaHI BsaWI BseAI BsiWI
BspDI BspEI BspMI BssHII BstEII
BstPI ClaI EagI EclXI Eco47III
Eco52I EcoO65I EcoRV FseI HaeII
Hin1I HpaI KasI KpnI KspI
MluI MroI NarI NheI NotI
NruI PacI PaeR7I PinAI PmeI
Psp1406I SacII SalI ScaI SfiI
SfoI SgrAI SnaBI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AflII 3921 CTTAAG C^TTAAG
ApaI 407 GGGCCC GGGCC^C
AspI 1919 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1590 TGCGCA TGC^GCA
AvrII 3633 CCTAGG C^CTAGG
BanI 3597 GGTGCC G^G[CT][GA]CC
BciVI 174 GTATCC GTATCC(6/5)
BfrI 3921 CTTAAG C^TTAAG
BglI 1762 GCCAACAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 3633 CCTAGG C^CTAGG
BlpI 2960 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2960 GCTGAGC GC^T[ACGT]AGC
BsaBI 4776 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 436 CGATCG CG[GA][CT]^CG
BsmBI 609 GAGACG CGTCTC(1/5)
BspHI 1013 TCATGA T^CATGA
BspLU11I 1640 ACATGT A^CATGT
BsrBI 4335 CCGCTC CCGCTC(-3/-3)
BsrDI 198 GCAATG GCAATG(2/0)
BssSI 3855 CACGAG CACGAG(-5/-1)
Bst1107I 1223 GTATAC GTA^TAC
BstAPI 3926 GCAGATTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2203 TTCGAA TT^CGAA
BstXI 1220 CCAGTATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1223 GTATAC GTA^TAC
CelII 2960 GCTGAGC GC^T[ACGT]AGC
CpoI 4528 CGGACCG CG^G[AT]CCG
DraIII 61 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4530 GACCGATGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3069 CCTACTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 3123 ATGCAT ATGCA^T
FspI 1590 TGCGCA TGC^GCA
HindIII 2179 AAGCTT A^AGCTT
MamI 4776 GATATTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2529 CAATTG C^AATTG
MunI 2529 CAATTG C^AATTG
NaeI 4368 GCCGGC GCC^GGC
NcoI 739 CCATGG C^CATGG
NgoMIV 4368 GCCGGC G^CCGGC
NsiI 3123 ATGCAT ATGCA^T
NspV 2203 TTCGAA TT^CGAA
PflFI 1919 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1788 GACCATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 436 CGATCG CGAT^CG
RcaI 1013 TCATGA T^CATGA
RsrII 4528 CGGACCG CG^G[AT]CCG
SbfI 2672 CCTGCAGG CCTGCA^GG
SfuI 2203 TTCGAA TT^CGAA
SmaI 94 CCCGGG CCC^GGG
SpeI 4911 ACTAGT A^CTAGT
SphI 1587 GCATGC GCATG^C
Sse8387I 2672 CCTGCAGG CCTGCA^GG
Tth111I 1919 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 94 CCCGGG C^CCGGG
XmaI 94 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1223 GTATAC GT^[AC][GT]AC
2431 GTCTAC
AhdI 1315 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2766 GACCTGGAGTC
AspEI 1315 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2766 GACCTGGAGTC
AvaI 94 CCCGGG C^[CT]CG[GA]G
901 CCCGAG
BalI 234 TGGCCA TGG^CCA
4132 TGGCCA
BbrPI 3377 CACGTG CAC^GTG
3422 CACGTG
BsaI 1385 GAGACC GGTCTC(1/5)
1598 GAGACC
BsmI 911 GAATGC GAATGC(1/-1)
4683 GCATTC
BsoBI 94 CCCGGG C^[CT]CG[GA]G
901 CCCGAG
BsrFI 213 ACCGGC [GA]^CCGG[CT]
4368 GCCGGC
Cfr10I 213 ACCGGC [GA]^CCGG[CT]
4368 GCCGGC
DraI 3304 TTTAAA TTT^AAA
3759 TTTAAA
DsaI 739 CCATGG C^C[GA][CT]GG
3402 CCGTGG
Eam1105I 1315 GACGTTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2766 GACCTGGAGTC
EcoRI 3471 GAATTC G^AATTC
3643 GAATTC
HincII 1795 GTCAAC GT[CT]^[GA]AC
2661 GTCAAC
HindII 1795 GTCAAC GT[CT]^[GA]AC
2661 GTCAAC
MluNI 234 TGGCCA TGG^CCA
4132 TGGCCA
MscI 234 TGGCCA TGG^CCA
4132 TGGCCA
MslI 3658 CATCCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4041 CATCCTTGTG
NdeI 3662 CATATG CA^TATG
3875 CATATG
NspI 1587 GCATGC [GA]CATG^[CT]
1640 ACATGT
PmaCI 3377 CACGTG CAC^GTG
3422 CACGTG
PmlI 3377 CACGTG CAC^GTG
3422 CACGTG
SexAI 859 ACCAGGT A^CC[AT]GGT
1828 ACCAGGT
StuI 2979 AGGCCT AGG^CCT
3077 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaII BanII
BbsI BbvI BclI BcnI BfaI
BglII BmyI BpmI BpuAI BsaAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshBI
PstI PvuII RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII