################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AccIII AclI AflII AgeI Aor51HI AscI AviII AvrII BbeI BbrPI BbvCI BciVI BclI BfrI BlnI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BspEI BsrFI BstAPI Cfr10I CpoI EagI EclXI Eco47III Eco52I EcoNI EcoT22I FbaI FseI FspI HaeII KasI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmlI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AatII 70 GACGTC GACGT^C Acc65I 2946 GGTACC G^GTACC AcyI 70 GACGTC G[GA]^CG[CT]C AhdI 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2903 GTGCAC G^TGCAC ApaI 306 GGGCCC GGGCC^C ApaLI 2903 GTGCAC G^TGCAC AseI 578 ATTAAT AT^TAAT AsnI 578 ATTAAT AT^TAAT Asp700I 2469 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2946 GGTACC G^GTACC AspEI 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1038 CCCGGG C^[CT]CG[GA]G BalI 1416 TGGCCA TGG^CCA BanII 306 GGGCCC G[GA]GC[CT]^C BcgI 495 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1302 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 70 GACGTC G[GA]^CG[CT]C BsaI 1286 GAGACC GGTCTC(1/5) BsoBI 1038 CCCGGG C^[CT]CG[GA]G BspDI 2012 ATCGAT AT^CGAT BspMI 2397 ACCTGC ACCTGC(4/8) BsrBI 2306 GAGCGG CCGCTC(-3/-3) BssHII 3630 GCGCGC G^CGCGC BssSI 3695 CTCGTG CACGAG(-5/-1) Bst1107I 1260 GTATAC GTA^TAC BstBI 1380 TTCGAA TT^CGAA BstEII 1880 GGTTACC G^GT[ACGT]ACC BstPI 1880 GGTTACC G^GT[ACGT]ACC BstXI 2775 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1260 GTATAC GTA^TAC ClaI 2012 ATCGAT AT^CGAT DrdI 2801 GACTATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1195 CCATGG C^C[GA][CT]GG EaeI 1416 TGGCCA [CT]^GGCC[GA] Eam1105I 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 1880 GGTTACC G^GT[ACGT]ACC Hin1I 70 GACGTC G[GA]^CG[CT]C KpnI 2946 GGTACC GGTAC^C MfeI 3300 CAATTG C^AATTG MluNI 1416 TGGCCA TGG^CCA MscI 1416 TGGCCA TGG^CCA MunI 3300 CAATTG C^AATTG NcoI 1195 CCATGG C^CATGG NdeI 595 CATATG CA^TATG NheI 1630 GCTAGC G^CTAGC NspV 1380 TTCGAA TT^CGAA PflFI 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2742 CCAATGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmeI 3293 GTTTAAAC GTTT^AAAC PshBI 578 ATTAAT AT^TAAT SapI 1268 GAAGAGC GCTCTTC(1/4) ScaI 2508 AGTACT AGT^ACT SfuI 1380 TTCGAA TT^CGAA SmaI 1038 CCCGGG CCC^GGG SpeI 1985 ACTAGT A^CTAGT StuI 2072 AGGCCT AGG^CCT Tth111I 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2742 CCAATGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 1038 CCCGGG C^CCGGG XmaI 1038 CCCGGG C^CCGGG XmnI 2469 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccII 112 CGCG CG^CG 3631 CGCG BamHI 1131 GGATCC G^GATCC 1843 GGATCC BanI 2343 GGCACC G^G[CT][GA]CC 2946 GGTACC BglII 2001 AGATCT A^GATCT 4070 AGATCT BlpI 1252 GCTGAGC GC^T[ACGT]AGC 3343 GCTTAGC Bpu1102I 1252 GCTGAGC GC^T[ACGT]AGC 3343 GCTTAGC BsmFI 1041 GGGAC GGGAC(10/14) 4020 GTCCC BstUI 112 CGCG CG^CG 3631 CGCG CelII 1252 GCTGAGC GC^T[ACGT]AGC 3343 GCTTAGC DraIII 2530 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG 3917 CACTTAGTG EarI 1323 CTCTTC CTCTTC(1/4) 1268 GAAGAG EcoRI 83 GAATTC G^AATTC 2132 GAATTC EcoRV 869 GATATC GAT^ATC 1907 GATATC FauI 311 CCCGC CCCGC(4/6) 1814 CCCGC HapII 1039 CCGG C^CGG 2677 CCGG HgaI 2325 GACGC GACGC(5/10) 3638 GACGC HpaI 548 GTTAAC GTT^AAC 3262 GTTAAC HpaII 1039 CCGG C^CGG 2677 CCGG Ksp632I 1323 CTCTTC CTCTTC(1/4) 1268 GAAGAG MspI 1039 CCGG C^CGG 2677 CCGG MvnI 112 CGCG CG^CG 3631 CGCG PpuMI 645 AGGTCCT [GA]G^G[AT]CC[CT] 1041 GGGACCT XcmI 1135 CCAGGGGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3006 CCATGAGGGTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AccI AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI Bsp1286I Bsp1407I BspHI BspLU11I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII Eco81I EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII