##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AccIII AclI AflII AgeI Aor51HI
AscI AviII AvrII BbeI BbrPI
BbvCI BciVI BclI BfrI BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspEI BsrFI BstAPI
Cfr10I CpoI EagI EclXI Eco47III
Eco52I EcoNI EcoT22I FbaI FseI
FspI HaeII KasI KspI MamI
MluI MroI NaeI NarI NgoMIV
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmlI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgrAI
SnaBI SphI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 70 GACGTC GACGT^C
Acc65I 2946 GGTACC G^GTACC
AcyI 70 GACGTC G[GA]^CG[CT]C
AhdI 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2903 GTGCAC G^TGCAC
ApaI 306 GGGCCC GGGCC^C
ApaLI 2903 GTGCAC G^TGCAC
AseI 578 ATTAAT AT^TAAT
AsnI 578 ATTAAT AT^TAAT
Asp700I 2469 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2946 GGTACC G^GTACC
AspEI 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1038 CCCGGG C^[CT]CG[GA]G
BalI 1416 TGGCCA TGG^CCA
BanII 306 GGGCCC G[GA]GC[CT]^C
BcgI 495 GCAGTGACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1302 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 70 GACGTC G[GA]^CG[CT]C
BsaI 1286 GAGACC GGTCTC(1/5)
BsoBI 1038 CCCGGG C^[CT]CG[GA]G
BspDI 2012 ATCGAT AT^CGAT
BspMI 2397 ACCTGC ACCTGC(4/8)
BsrBI 2306 GAGCGG CCGCTC(-3/-3)
BssHII 3630 GCGCGC G^CGCGC
BssSI 3695 CTCGTG CACGAG(-5/-1)
Bst1107I 1260 GTATAC GTA^TAC
BstBI 1380 TTCGAA TT^CGAA
BstEII 1880 GGTTACC G^GT[ACGT]ACC
BstPI 1880 GGTTACC G^GT[ACGT]ACC
BstXI 2775 CCACCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1260 GTATAC GTA^TAC
ClaI 2012 ATCGAT AT^CGAT
DrdI 2801 GACTATGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1195 CCATGG C^C[GA][CT]GG
EaeI 1416 TGGCCA [CT]^GGCC[GA]
Eam1105I 2140 GACTCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1880 GGTTACC G^GT[ACGT]ACC
Hin1I 70 GACGTC G[GA]^CG[CT]C
KpnI 2946 GGTACC GGTAC^C
MfeI 3300 CAATTG C^AATTG
MluNI 1416 TGGCCA TGG^CCA
MscI 1416 TGGCCA TGG^CCA
MunI 3300 CAATTG C^AATTG
NcoI 1195 CCATGG C^CATGG
NdeI 595 CATATG CA^TATG
NheI 1630 GCTAGC G^CTAGC
NspV 1380 TTCGAA TT^CGAA
PflFI 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2742 CCAATGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmeI 3293 GTTTAAAC GTTT^AAAC
PshBI 578 ATTAAT AT^TAAT
SapI 1268 GAAGAGC GCTCTTC(1/4)
ScaI 2508 AGTACT AGT^ACT
SfuI 1380 TTCGAA TT^CGAA
SmaI 1038 CCCGGG CCC^GGG
SpeI 1985 ACTAGT A^CTAGT
StuI 2072 AGGCCT AGG^CCT
Tth111I 1242 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2742 CCAATGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1038 CCCGGG C^CCGGG
XmaI 1038 CCCGGG C^CCGGG
XmnI 2469 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 112 CGCG CG^CG
3631 CGCG
BamHI 1131 GGATCC G^GATCC
1843 GGATCC
BanI 2343 GGCACC G^G[CT][GA]CC
2946 GGTACC
BglII 2001 AGATCT A^GATCT
4070 AGATCT
BlpI 1252 GCTGAGC GC^T[ACGT]AGC
3343 GCTTAGC
Bpu1102I 1252 GCTGAGC GC^T[ACGT]AGC
3343 GCTTAGC
BsmFI 1041 GGGAC GGGAC(10/14)
4020 GTCCC
BstUI 112 CGCG CG^CG
3631 CGCG
CelII 1252 GCTGAGC GC^T[ACGT]AGC
3343 GCTTAGC
DraIII 2530 CACTTCGTG CAC[ACGT][ACGT][ACGT]^GTG
3917 CACTTAGTG
EarI 1323 CTCTTC CTCTTC(1/4)
1268 GAAGAG
EcoRI 83 GAATTC G^AATTC
2132 GAATTC
EcoRV 869 GATATC GAT^ATC
1907 GATATC
FauI 311 CCCGC CCCGC(4/6)
1814 CCCGC
HapII 1039 CCGG C^CGG
2677 CCGG
HgaI 2325 GACGC GACGC(5/10)
3638 GACGC
HpaI 548 GTTAAC GTT^AAC
3262 GTTAAC
HpaII 1039 CCGG C^CGG
2677 CCGG
Ksp632I 1323 CTCTTC CTCTTC(1/4)
1268 GAAGAG
MspI 1039 CCGG C^CGG
2677 CCGG
MvnI 112 CGCG CG^CG
3631 CGCG
PpuMI 645 AGGTCCT [GA]G^G[AT]CC[CT]
1041 GGGACCT
XcmI 1135 CCAGGGGAATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3006 CCATGAGGGTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmI Bsp1286I Bsp1407I BspHI
BspLU11I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HincII HindII HindIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII