##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
Acc65I AccI AccIII AclI AflIII
AgeI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BcgI BclI BlnI BsaWI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstEII BstPI BstZ17I ClaI CpoI
DrdI EcoO65I EcoRV FauI FbaI
FseI FspI HpaI KasI KpnI
KspI MluI MroI NarI NcoI
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
RsrII SacII SalI SbfI ScaI
SfiI SfoI SgrAI SnaBI Sse8387I
SspBI SwaI Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AhdI 4160 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 853 GTGCAC G^TGCAC
AocI 828 CCTGAGG CC^T[ACGT]AGG
ApaLI 853 GTGCAC G^TGCAC
Asp700I 2358 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4160 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 3602 GGATCC G^GATCC
BanII 418 GAGCTC G[GA]GC[CT]^C
BbrPI 4170 CACGTG CAC^GTG
BglI 2139 GCCTTGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2834 AGATCT A^GATCT
BsaI 4670 GAGACC GGTCTC(1/5)
BspHI 155 TCATGA T^CATGA
BspMI 839 ACCTGC ACCTGC(4/8)
BsrFI 3699 GCCGGC [GA]^CCGG[CT]
BstBI 2365 TTCGAA TT^CGAA
BstXI 3634 CCACAGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 828 CCTGAGG CC^T[ACGT]AGG
Cfr10I 3699 GCCGGC [GA]^CCGG[CT]
DraI 1911 TTTAAA TTT^AAA
DraII 3570 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 3971 CGGCCG C^GGCCG
Eam1105I 4160 GACTCCGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3971 CGGCCG C^GGCCG
Eco52I 3971 CGGCCG C^GGCCG
Eco81I 828 CCTGAGG CC^T[ACGT]AGG
EcoNI 3899 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 3570 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 1683 ATGCAT ATGCA^T
MfeI 3174 CAATTG C^AATTG
MunI 3174 CAATTG C^AATTG
NaeI 3699 GCCGGC GCC^GGC
NgoMIV 3699 GCCGGC G^CCGGC
NsiI 1683 ATGCAT ATGCA^T
NspV 2365 TTCGAA TT^CGAA
PflMI 1665 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4170 CACGTG CAC^GTG
PmlI 4170 CACGTG CAC^GTG
PpuMI 3570 AGGTCCT [GA]G^G[AT]CC[CT]
PvuI 948 CGATCG CGAT^CG
RcaI 155 TCATGA T^CATGA
SacI 418 GAGCTC GAGCT^C
SfuI 2365 TTCGAA TT^CGAA
SpeI 977 ACTAGT A^CTAGT
SphI 2429 GCATGC GCATG^C
StuI 4255 AGGCCT AGG^CCT
Van91I 1665 CCAGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1397 TCTAGA T^CTAGA
XmnI 2358 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 46 enzymes) #
###################################
AatII 658 GACGTC GACGT^C
875 GACGTC
AccII 1788 CGCG CG^CG
4070 CGCG
AflII 413 CTTAAG C^TTAAG
992 CTTAAG
AlwNI 748 CAGTAACTG CAG[ACGT][ACGT][ACGT]^CTG
2671 CAGAGCCTG
Aor51HI 142 AGCGCT AGC^GCT
2291 AGCGCT
AspHI 418 GAGCTC G[AT]GC[AT]^C
853 GTGCAC
BalI 3499 TGGCCA TGG^CCA
4132 TGGCCA
BanI 394 GGCACC G^G[CT][GA]CC
3684 GGCACC
BciVI 2578 GTATCC GTATCC(6/5)
3670 GTATCC
BfrI 413 CTTAAG C^TTAAG
992 CTTAAG
BlpI 2304 GCTCAGC GC^T[ACGT]AGC
4781 GCTGAGC
BmyI 418 GAGCTC G[AGT]GC[ACT]^C
853 GTGCAC
Bpu1102I 2304 GCTCAGC GC^T[ACGT]AGC
4781 GCTGAGC
BsaAI 3532 TACGTG [CT]AC^GT[GA]
4170 CACGTG
BsaBI 2738 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3483 GATGAGAATC
BsiEI 948 CGATCG CG[GA][CT]^CG
3971 CGGCCG
BsiHKAI 418 GAGCTC G[AT]GC[AT]^C
853 GTGCAC
BsmI 2362 GCATTC GAATGC(1/-1)
3837 GCATTC
Bsp1286I 418 GAGCTC G[AGT]GC[ACT]^C
853 GTGCAC
BsrDI 2494 GCAATG GCAATG(2/0)
3067 GCAATG
BstAPI 747 GCAGTAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3097 GCAATAACTGC
BstUI 1788 CGCG CG^CG
4070 CGCG
BstYI 2834 AGATCT [GA]^GATC[CT]
3602 GGATCC
CelII 2304 GCTCAGC GC^T[ACGT]AGC
4781 GCTGAGC
CfoI 143 GCGC GCG^C
2292 GCGC
DsaI 13 CCGTGG C^C[GA][CT]GG
3680 CCACGG
Eco47III 142 AGCGCT AGC^GCT
2291 AGCGCT
HaeII 142 AGCGCT [GA]GCGC^[CT]
2291 AGCGCT
HgaI 3696 GACGC GACGC(5/10)
1613 GCGTC
HhaI 143 GCGC GCG^C
2292 GCGC
HinP1I 143 GCGC G^CGC
2292 GCGC
MamI 2738 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3483 GATGAGAATC
MflI 2834 AGATCT [GA]^GATC[CT]
3602 GGATCC
MluNI 3499 TGGCCA TGG^CCA
4132 TGGCCA
MscI 3499 TGGCCA TGG^CCA
4132 TGGCCA
MvnI 1788 CGCG CG^CG
4070 CGCG
NdeI 1025 CATATG CA^TATG
3657 CATATG
NheI 1385 GCTAGC G^CTAGC
3852 GCTAGC
NspI 1681 ACATGC [GA]CATG^[CT]
2429 GCATGC
PvuII 3619 CAGCTG CAG^CTG
4005 CAGCTG
SexAI 740 ACCTGGT A^CC[AT]GGT
3952 ACCAGGT
SmaI 2254 CCCGGG CCC^GGG
4215 CCCGGG
SspI 2927 AATATT AAT^ATT
4482 AATATT
XhoII 2834 AGATCT [GA]^GATC[CT]
3602 GGATCC
XmaCI 2254 CCCGGG C^CCGGG
4215 CCCGGG
XmaI 2254 CCCGGG C^CCGGG
4215 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaI AvaII BbsI
BbvCI BbvI BcnI BfaI BpmI
BpuAI BsaHI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsoBI
BsrI BssKI BssSI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraIII
EaeI EarI EcoRI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII Hin1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I