##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AhdI AscI
Asp718I AspEI AspI AvrII BbeI
BcgI BciVI BglII BlnI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspHI BsrGI BssHII BssSI
BstBI ClaI CpoI EagI Eam1105I
EclXI Eco52I EcoRI EcoRV FseI
HindIII HpaI KasI KpnI KspI
MamI MluI MroI NarI NdeI
NotI NruI NspV PacI PaeR7I
PflFI PmeI PshAI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccII 648 CGCG CG^CG
AclI 1792 AACGTT AA^CGTT
AflII 4287 CTTAAG C^TTAAG
AgeI 3832 ACCGGT A^CCGGT
AocI 3257 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1628 AGCGCT AGC^GCT
Asp700I 3128 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3221 TGCGCA TGC^GCA
BamHI 4282 GGATCC G^GATCC
BbrPI 2110 CACGTG CAC^GTG
BbvCI 3137 CCTCAGC CCTCAGC(-5/-2)
BclI 758 TGATCA T^GATCA
BfrI 4287 CTTAAG C^TTAAG
BglI 3389 GCCATTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 3832 ACCGGT [AT]^CCGG[AT]
BsmBI 689 CGTCTC CGTCTC(1/5)
BspLU11I 4371 ACATGT A^CATGT
Bst1107I 2343 GTATAC GTA^TAC
BstUI 648 CGCG CG^CG
BstXI 1853 CCAAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 2343 GTATAC GTA^TAC
Bsu36I 3257 CCTCAGG CC^T[ACGT]AGG
DrdI 2243 GACGAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1628 AGCGCT AGC^GCT
Eco81I 3257 CCTCAGG CC^T[ACGT]AGG
EcoNI 3149 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 758 TGATCA T^GATCA
FspI 3221 TGCGCA TGC^GCA
MfeI 1734 CAATTG C^AATTG
MunI 1734 CAATTG C^AATTG
MvnI 648 CGCG CG^CG
NaeI 2655 GCCGGC GCC^GGC
NgoMIV 2655 GCCGGC G^CCGGC
NheI 1639 GCTAGC G^CTAGC
PinAI 3832 ACCGGT A^CCGGT
PmaCI 2110 CACGTG CAC^GTG
PmlI 2110 CACGTG CAC^GTG
Psp1406I 1792 AACGTT AA^CGTT
SapI 3962 GCTCTTC GCTCTTC(1/4)
SexAI 278 ACCTGGT A^CC[AT]GGT
SmaI 3209 CCCGGG CCC^GGG
SpeI 4609 ACTAGT A^CTAGT
StuI 1100 AGGCCT AGG^CCT
XmaCI 3209 CCCGGG C^CCGGG
XmaI 3209 CCCGGG C^CCGGG
XmnI 3128 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 1228 GTCTAC GT^[AC][GT]AC
2343 GTATAC
AflIII 2186 ACGTGT A^C[GA][CT]GT
4371 ACATGT
Alw44I 933 GTGCAC G^TGCAC
3092 GTGCAC
ApaLI 933 GTGCAC G^TGCAC
3092 GTGCAC
AseI 2412 ATTAAT AT^TAAT
4657 ATTAAT
AsnI 2412 ATTAAT AT^TAAT
4657 ATTAAT
AvaI 1193 CTCGGG C^[CT]CG[GA]G
3209 CCCGGG
BanI 1609 GGCACC G^G[CT][GA]CC
1718 GGCACC
BbsI 3850 GAAGAC GAAGAC(2/6)
4778 GTCTTC
BlpI 264 GCTCAGC GC^T[ACGT]AGC
1624 GCTGAGC
Bpu1102I 264 GCTCAGC GC^T[ACGT]AGC
1624 GCTGAGC
BpuAI 3850 GAAGAC GAAGAC(2/6)
4778 GTCTTC
BsaAI 2110 CACGTG [CT]AC^GT[GA]
3095 CACGTA
BsaI 275 GAGACC GGTCTC(1/5)
3835 GGTCTC
BsoBI 1193 CTCGGG C^[CT]CG[GA]G
3209 CCCGGG
BsrBI 1710 CCGCTC CCGCTC(-3/-3)
1804 GAGCGG
BsrDI 999 CATTGC GCAATG(2/0)
2808 GCAATG
BstEII 1479 GGTGACC G^GT[ACGT]ACC
4591 GGTTACC
BstPI 1479 GGTGACC G^GT[ACGT]ACC
4591 GGTTACC
BstYI 2566 GGATCT [GA]^GATC[CT]
4282 GGATCC
CelII 264 GCTCAGC GC^T[ACGT]AGC
1624 GCTGAGC
DraIII 2007 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
4544 CACAGGGTG
EcoO65I 1479 GGTGACC G^GT[ACGT]ACC
4591 GGTTACC
HincII 301 GTCAAC GT[CT]^[GA]AC
3415 GTTGAC
HindII 301 GTCAAC GT[CT]^[GA]AC
3415 GTTGAC
MflI 2566 GGATCT [GA]^GATC[CT]
4282 GGATCC
NcoI 1703 CCATGG C^CATGG
2016 CCATGG
PflMI 14 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
453 CCAGAAGGTGG
PpuMI 74 GGGTCCT [GA]G^G[AT]CC[CT]
3281 AGGACCT
PshBI 2412 ATTAAT AT^TAAT
4657 ATTAAT
SacI 2585 GAGCTC GAGCT^C
2666 GAGCTC
SmlI 4171 CTTGAG C^T[CT][GA]AG
4287 CTTAAG
SphI 921 GCATGC GCATG^C
4554 GCATGC
Van91I 14 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
453 CCAGAAGGTGG
XhoII 2566 GGATCT [GA]^GATC[CT]
4282 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BspMI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI