################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AhdI AscI Asp718I AspEI AspI AvrII BbeI BcgI BciVI BglII BlnI BsaBI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII BssSI BstBI ClaI CpoI EagI Eam1105I EclXI Eco52I EcoRI EcoRV FseI HindIII HpaI KasI KpnI KspI MamI MluI MroI NarI NdeI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 648 CGCG CG^CG AclI 1792 AACGTT AA^CGTT AflII 4287 CTTAAG C^TTAAG AgeI 3832 ACCGGT A^CCGGT AocI 3257 CCTCAGG CC^T[ACGT]AGG Aor51HI 1628 AGCGCT AGC^GCT Asp700I 3128 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 3221 TGCGCA TGC^GCA BamHI 4282 GGATCC G^GATCC BbrPI 2110 CACGTG CAC^GTG BbvCI 3137 CCTCAGC CCTCAGC(-5/-2) BclI 758 TGATCA T^GATCA BfrI 4287 CTTAAG C^TTAAG BglI 3389 GCCATTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaWI 3832 ACCGGT [AT]^CCGG[AT] BsmBI 689 CGTCTC CGTCTC(1/5) BspLU11I 4371 ACATGT A^CATGT Bst1107I 2343 GTATAC GTA^TAC BstUI 648 CGCG CG^CG BstXI 1853 CCAAAATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 2343 GTATAC GTA^TAC Bsu36I 3257 CCTCAGG CC^T[ACGT]AGG DrdI 2243 GACGAAGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1628 AGCGCT AGC^GCT Eco81I 3257 CCTCAGG CC^T[ACGT]AGG EcoNI 3149 CCTCAAATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 758 TGATCA T^GATCA FspI 3221 TGCGCA TGC^GCA MfeI 1734 CAATTG C^AATTG MunI 1734 CAATTG C^AATTG MvnI 648 CGCG CG^CG NaeI 2655 GCCGGC GCC^GGC NgoMIV 2655 GCCGGC G^CCGGC NheI 1639 GCTAGC G^CTAGC PinAI 3832 ACCGGT A^CCGGT PmaCI 2110 CACGTG CAC^GTG PmlI 2110 CACGTG CAC^GTG Psp1406I 1792 AACGTT AA^CGTT SapI 3962 GCTCTTC GCTCTTC(1/4) SexAI 278 ACCTGGT A^CC[AT]GGT SmaI 3209 CCCGGG CCC^GGG SpeI 4609 ACTAGT A^CTAGT StuI 1100 AGGCCT AGG^CCT XmaCI 3209 CCCGGG C^CCGGG XmaI 3209 CCCGGG C^CCGGG XmnI 3128 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 1228 GTCTAC GT^[AC][GT]AC 2343 GTATAC AflIII 2186 ACGTGT A^C[GA][CT]GT 4371 ACATGT Alw44I 933 GTGCAC G^TGCAC 3092 GTGCAC ApaLI 933 GTGCAC G^TGCAC 3092 GTGCAC AseI 2412 ATTAAT AT^TAAT 4657 ATTAAT AsnI 2412 ATTAAT AT^TAAT 4657 ATTAAT AvaI 1193 CTCGGG C^[CT]CG[GA]G 3209 CCCGGG BanI 1609 GGCACC G^G[CT][GA]CC 1718 GGCACC BbsI 3850 GAAGAC GAAGAC(2/6) 4778 GTCTTC BlpI 264 GCTCAGC GC^T[ACGT]AGC 1624 GCTGAGC Bpu1102I 264 GCTCAGC GC^T[ACGT]AGC 1624 GCTGAGC BpuAI 3850 GAAGAC GAAGAC(2/6) 4778 GTCTTC BsaAI 2110 CACGTG [CT]AC^GT[GA] 3095 CACGTA BsaI 275 GAGACC GGTCTC(1/5) 3835 GGTCTC BsoBI 1193 CTCGGG C^[CT]CG[GA]G 3209 CCCGGG BsrBI 1710 CCGCTC CCGCTC(-3/-3) 1804 GAGCGG BsrDI 999 CATTGC GCAATG(2/0) 2808 GCAATG BstEII 1479 GGTGACC G^GT[ACGT]ACC 4591 GGTTACC BstPI 1479 GGTGACC G^GT[ACGT]ACC 4591 GGTTACC BstYI 2566 GGATCT [GA]^GATC[CT] 4282 GGATCC CelII 264 GCTCAGC GC^T[ACGT]AGC 1624 GCTGAGC DraIII 2007 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG 4544 CACAGGGTG EcoO65I 1479 GGTGACC G^GT[ACGT]ACC 4591 GGTTACC HincII 301 GTCAAC GT[CT]^[GA]AC 3415 GTTGAC HindII 301 GTCAAC GT[CT]^[GA]AC 3415 GTTGAC MflI 2566 GGATCT [GA]^GATC[CT] 4282 GGATCC NcoI 1703 CCATGG C^CATGG 2016 CCATGG PflMI 14 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 453 CCAGAAGGTGG PpuMI 74 GGGTCCT [GA]G^G[AT]CC[CT] 3281 AGGACCT PshBI 2412 ATTAAT AT^TAAT 4657 ATTAAT SacI 2585 GAGCTC GAGCT^C 2666 GAGCTC SmlI 4171 CTTGAG C^T[CT][GA]AG 4287 CTTAAG SphI 921 GCATGC GCATG^C 4554 GCATGC Van91I 14 CCAGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 453 CCAGAAGGTGG XhoII 2566 GGATCT [GA]^GATC[CT] 4282 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI