##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Aor51HI AscI AspI AviII BamHI
BbeI BbrPI BglI BglII BsaHI
BseAI BsiEI BsmBI Bsp1407I BspDI
BspEI BsrFI BsrGI BssHII Bst1107I
BstZ17I Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I EcoRV
FauI FseI FspI Hin1I KasI
KspI MluI MroI NaeI NarI
NdeI NgoMIV NotI PacI PflFI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI StuI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 2342 GGTACC G^GTACC
AflII 4235 CTTAAG C^TTAAG
AflIII 4876 ACATGT A^C[GA][CT]GT
AhdI 1931 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4079 GTGCAC G^TGCAC
ApaI 3252 GGGCCC GGGCC^C
ApaLI 4079 GTGCAC G^TGCAC
AseI 5305 ATTAAT AT^TAAT
AsnI 5305 ATTAAT AT^TAAT
Asp718I 2342 GGTACC G^GTACC
AspEI 1931 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4090 CCTAGG C^CTAGG
BbvCI 1813 GCTGAGG CCTCAGC(-5/-2)
BcgI 2259 CGACAGAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4235 CTTAAG C^TTAAG
BlnI 4090 CCTAGG C^CTAGG
BlpI 1415 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1415 GCTGAGC GC^T[ACGT]AGC
BsaBI 3067 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 392 GAGACC GGTCTC(1/5)
BsiWI 4278 CGTACG C^GTACG
BspLU11I 4876 ACATGT A^CATGT
BsrBI 3779 GAGCGG CCGCTC(-3/-3)
BstAPI 1060 GCAAGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2081 TTCGAA TT^CGAA
CelII 1415 GCTGAGC GC^T[ACGT]AGC
DrdI 3916 GACCACAACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1931 GACAGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 4847 ATGCAT ATGCA^T
HpaI 5004 GTTAAC GTT^AAC
KpnI 2342 GGTACC GGTAC^C
MamI 3067 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 1227 CCATGG C^CATGG
NheI 3106 GCTAGC G^CTAGC
NruI 96 TCGCGA TCG^CGA
NsiI 4847 ATGCAT ATGCA^T
NspV 2081 TTCGAA TT^CGAA
PflMI 3115 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 5305 ATTAAT AT^TAAT
SacI 2963 GAGCTC GAGCT^C
SapI 1616 GAAGAGC GCTCTTC(1/4)
SfuI 2081 TTCGAA TT^CGAA
SmaI 1128 CCCGGG CCC^GGG
SphI 2999 GCATGC GCATG^C
SspI 1526 AATATT AAT^ATT
Van91I 3115 CCAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 592 TCTAGA T^CTAGA
XmaCI 1128 CCCGGG C^CCGGG
XmaI 1128 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1928 GTAGAC GT^[AC][GT]AC
4063 GTCTAC
AccII 97 CGCG CG^CG
3159 CGCG
AocI 485 CCTCAGG CC^T[ACGT]AGG
4130 CCTAAGG
BalI 2164 TGGCCA TGG^CCA
2379 TGGCCA
BanI 2342 GGTACC G^G[CT][GA]CC
3023 GGTGCC
BciVI 1639 GGATAC GTATCC(6/5)
3588 GGATAC
BclI 1828 TGATCA T^GATCA
4615 TGATCA
BsaAI 2990 TACGTG [CT]AC^GT[GA]
5338 CACGTA
BsaWI 541 ACCGGA [AT]^CCGG[AT]
3262 TCCGGT
BspMI 4042 ACCTGC ACCTGC(4/8)
4606 ACCTGC
BssSI 3302 CACGAG CACGAG(-5/-1)
3849 CTCGTG
BstEII 3184 GGTGACC G^GT[ACGT]ACC
3339 GGTTACC
BstPI 3184 GGTGACC G^GT[ACGT]ACC
3339 GGTTACC
BstUI 97 CGCG CG^CG
3159 CGCG
BstYI 2146 GGATCT [GA]^GATC[CT]
3412 GGATCT
Bsu36I 485 CCTCAGG CC^T[ACGT]AGG
4130 CCTAAGG
EaeI 2164 TGGCCA [CT]^GGCC[GA]
2379 TGGCCA
Eco81I 485 CCTCAGG CC^T[ACGT]AGG
4130 CCTAAGG
EcoO65I 3184 GGTGACC G^GT[ACGT]ACC
3339 GGTTACC
EcoRI 472 GAATTC G^AATTC
5082 GAATTC
FbaI 1828 TGATCA T^GATCA
4615 TGATCA
HgaI 1003 GCGTC GACGC(5/10)
1402 GCGTC
HindIII 2305 AAGCTT A^AGCTT
2417 AAGCTT
MflI 2146 GGATCT [GA]^GATC[CT]
3412 GGATCT
MluNI 2164 TGGCCA TGG^CCA
2379 TGGCCA
MscI 2164 TGGCCA TGG^CCA
2379 TGGCCA
MspA1I 467 CAGCTG C[AC]G^C[GT]G
3900 CAGCTG
MvnI 97 CGCG CG^CG
3159 CGCG
PaeR7I 1550 CTCGAG C^TCGAG
4445 CTCGAG
PshAI 703 GACGGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5023 GACTCAAGTC
PvuII 467 CAGCTG CAG^CTG
3900 CAGCTG
ScaI 2062 AGTACT AGT^ACT
4470 AGTACT
XhoI 1550 CTCGAG C^TCGAG
4445 CTCGAG
XhoII 2146 GGATCT [GA]^GATC[CT]
3412 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MfeI
MnlI MseI MslI MspI MunI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XmnI