################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AccIII AclI AflII AgeI AscI AseI AsnI AvrII BbeI BbrPI BcgI BfrI BlnI BseAI BsiWI BspEI BssHII BstBI CpoI DraI DraIII EagI EclXI Eco52I EcoRV EcoT22I FseI HpaI KasI MluI MroI NarI NdeI NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AatII 1482 GACGTC GACGT^C Acc65I 3835 GGTACC G^GTACC Alw44I 2000 GTGCAC G^TGCAC AocI 3199 CCTTAGG CC^T[ACGT]AGG ApaLI 2000 GTGCAC G^TGCAC Asp718I 3835 GGTACC G^GTACC AviII 676 TGCGCA TGC^GCA BamHI 3785 GGATCC G^GATCC BclI 2095 TGATCA T^GATCA BglII 1592 AGATCT A^GATCT BsaBI 2798 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 964 ACCGGA [AT]^CCGG[AT] BsiEI 962 CGACCG CG[GA][CT]^CG BsmBI 2148 GAGACG CGTCTC(1/5) BspDI 2388 ATCGAT AT^CGAT BspLU11I 1810 ACATGT A^CATGT BsrFI 3907 GCCGGC [GA]^CCGG[CT] Bst1107I 3267 GTATAC GTA^TAC BstAPI 3431 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 3267 GTATAC GTA^TAC Bsu36I 3199 CCTTAGG CC^T[ACGT]AGG Cfr10I 3907 GCCGGC [GA]^CCGG[CT] ClaI 2388 ATCGAT AT^CGAT Eco81I 3199 CCTTAGG CC^T[ACGT]AGG EcoRI 2115 GAATTC G^AATTC FbaI 2095 TGATCA T^GATCA FspI 676 TGCGCA TGC^GCA KpnI 3835 GGTACC GGTAC^C KspI 2584 CCGCGG CCGC^GG MamI 2798 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 629 CAATTG C^AATTG MseI 208 TTAA T^TAA MunI 629 CAATTG C^AATTG NaeI 3907 GCCGGC GCC^GGC NgoMIV 3907 GCCGGC G^CCGGC PflMI 1428 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 873 GAGCTC GAGCT^C SacII 2584 CCGCGG CCGC^GG SapI 2192 GAAGAGC GCTCTTC(1/4) SbfI 614 CCTGCAGG CCTGCA^GG ScaI 1855 AGTACT AGT^ACT SexAI 2395 ACCTGGT A^CC[AT]GGT SphI 2605 GCATGC GCATG^C Sse8387I 614 CCTGCAGG CCTGCA^GG Tru9I 208 TTAA T^TAA Van91I 1428 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2485 CCATCTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccII 6 CGCG CG^CG 2585 CGCG AflIII 1810 ACATGT A^C[GA][CT]GT 2275 ACGTGT Aor51HI 1903 AGCGCT AGC^GCT 2937 AGCGCT Asp700I 402 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1901 GAAGCGCTTC AspI 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3990 GACGCAGTC BbsI 1761 GAAGAC GAAGAC(2/6) 1485 GTCTTC BciVI 1716 GTATCC GTATCC(6/5) 2362 GTATCC BlpI 3032 GCTGAGC GC^T[ACGT]AGC 3681 GCTGAGC Bpu1102I 3032 GCTGAGC GC^T[ACGT]AGC 3681 GCTGAGC BpuAI 1761 GAAGAC GAAGAC(2/6) 1485 GTCTTC BsaAI 2175 TACGTG [CT]AC^GT[GA] 2274 TACGTG BsgI 108 GTGCAG GTGCAG(16/14) 1512 GTGCAG Bsp1407I 1813 TGTACA T^GTACA 2434 TGTACA BspHI 866 TCATGA T^CATGA 3367 TCATGA BsrGI 1813 TGTACA T^GTACA 2434 TGTACA BssSI 1338 CACGAG CACGAG(-5/-1) 1372 CTCGTG BstUI 6 CGCG CG^CG 2585 CGCG CelII 3032 GCTGAGC GC^T[ACGT]AGC 3681 GCTGAGC DrdI 2124 GACCAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2394 GACCTGGTGGTC Eco47III 1903 AGCGCT AGC^GCT 2937 AGCGCT EcoNI 445 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3529 CCTGGGGGAGG HaeII 1903 AGCGCT [GA]GCGC^[CT] 2937 AGCGCT HincII 795 GTCAAC GT[CT]^[GA]AC 3402 GTTGAC HindII 795 GTCAAC GT[CT]^[GA]AC 3402 GTTGAC MvnI 6 CGCG CG^CG 2585 CGCG NspI 1810 ACATGT [GA]CATG^[CT] 2605 GCATGC PflFI 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3990 GACGCAGTC RcaI 866 TCATGA T^CATGA 3367 TCATGA SmaI 2661 CCCGGG CCC^GGG 2875 CCCGGG SspBI 1813 TGTACA T^GTACA 2434 TGTACA TfiI 930 GATTC G^A[AT]TC 1883 GATTC Tth111I 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 3990 GACGCAGTC XmaCI 2661 CCCGGG C^CCGGG 2875 CCCGGG XmaI 2661 CCCGGG C^CCGGG 2875 CCCGGG XmnI 402 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1901 GAAGCGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AhdI AluI AlwI AlwNI ApaI ApoI AspEI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI Eam1105I EarI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PaeR7I PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoI XhoII