##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI AvrII BbeI BbrPI
BcgI BfrI BlnI BseAI BsiWI
BspEI BssHII BstBI CpoI DraI
DraIII EagI EclXI Eco52I EcoRV
EcoT22I FseI HpaI KasI MluI
MroI NarI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SalI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 1482 GACGTC GACGT^C
Acc65I 3835 GGTACC G^GTACC
Alw44I 2000 GTGCAC G^TGCAC
AocI 3199 CCTTAGG CC^T[ACGT]AGG
ApaLI 2000 GTGCAC G^TGCAC
Asp718I 3835 GGTACC G^GTACC
AviII 676 TGCGCA TGC^GCA
BamHI 3785 GGATCC G^GATCC
BclI 2095 TGATCA T^GATCA
BglII 1592 AGATCT A^GATCT
BsaBI 2798 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 964 ACCGGA [AT]^CCGG[AT]
BsiEI 962 CGACCG CG[GA][CT]^CG
BsmBI 2148 GAGACG CGTCTC(1/5)
BspDI 2388 ATCGAT AT^CGAT
BspLU11I 1810 ACATGT A^CATGT
BsrFI 3907 GCCGGC [GA]^CCGG[CT]
Bst1107I 3267 GTATAC GTA^TAC
BstAPI 3431 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 3267 GTATAC GTA^TAC
Bsu36I 3199 CCTTAGG CC^T[ACGT]AGG
Cfr10I 3907 GCCGGC [GA]^CCGG[CT]
ClaI 2388 ATCGAT AT^CGAT
Eco81I 3199 CCTTAGG CC^T[ACGT]AGG
EcoRI 2115 GAATTC G^AATTC
FbaI 2095 TGATCA T^GATCA
FspI 676 TGCGCA TGC^GCA
KpnI 3835 GGTACC GGTAC^C
KspI 2584 CCGCGG CCGC^GG
MamI 2798 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 629 CAATTG C^AATTG
MseI 208 TTAA T^TAA
MunI 629 CAATTG C^AATTG
NaeI 3907 GCCGGC GCC^GGC
NgoMIV 3907 GCCGGC G^CCGGC
PflMI 1428 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 873 GAGCTC GAGCT^C
SacII 2584 CCGCGG CCGC^GG
SapI 2192 GAAGAGC GCTCTTC(1/4)
SbfI 614 CCTGCAGG CCTGCA^GG
ScaI 1855 AGTACT AGT^ACT
SexAI 2395 ACCTGGT A^CC[AT]GGT
SphI 2605 GCATGC GCATG^C
Sse8387I 614 CCTGCAGG CCTGCA^GG
Tru9I 208 TTAA T^TAA
Van91I 1428 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2485 CCATCTGCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 6 CGCG CG^CG
2585 CGCG
AflIII 1810 ACATGT A^C[GA][CT]GT
2275 ACGTGT
Aor51HI 1903 AGCGCT AGC^GCT
2937 AGCGCT
Asp700I 402 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1901 GAAGCGCTTC
AspI 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGCAGTC
BbsI 1761 GAAGAC GAAGAC(2/6)
1485 GTCTTC
BciVI 1716 GTATCC GTATCC(6/5)
2362 GTATCC
BlpI 3032 GCTGAGC GC^T[ACGT]AGC
3681 GCTGAGC
Bpu1102I 3032 GCTGAGC GC^T[ACGT]AGC
3681 GCTGAGC
BpuAI 1761 GAAGAC GAAGAC(2/6)
1485 GTCTTC
BsaAI 2175 TACGTG [CT]AC^GT[GA]
2274 TACGTG
BsgI 108 GTGCAG GTGCAG(16/14)
1512 GTGCAG
Bsp1407I 1813 TGTACA T^GTACA
2434 TGTACA
BspHI 866 TCATGA T^CATGA
3367 TCATGA
BsrGI 1813 TGTACA T^GTACA
2434 TGTACA
BssSI 1338 CACGAG CACGAG(-5/-1)
1372 CTCGTG
BstUI 6 CGCG CG^CG
2585 CGCG
CelII 3032 GCTGAGC GC^T[ACGT]AGC
3681 GCTGAGC
DrdI 2124 GACCAGTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2394 GACCTGGTGGTC
Eco47III 1903 AGCGCT AGC^GCT
2937 AGCGCT
EcoNI 445 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3529 CCTGGGGGAGG
HaeII 1903 AGCGCT [GA]GCGC^[CT]
2937 AGCGCT
HincII 795 GTCAAC GT[CT]^[GA]AC
3402 GTTGAC
HindII 795 GTCAAC GT[CT]^[GA]AC
3402 GTTGAC
MvnI 6 CGCG CG^CG
2585 CGCG
NspI 1810 ACATGT [GA]CATG^[CT]
2605 GCATGC
PflFI 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGCAGTC
RcaI 866 TCATGA T^CATGA
3367 TCATGA
SmaI 2661 CCCGGG CCC^GGG
2875 CCCGGG
SspBI 1813 TGTACA T^GTACA
2434 TGTACA
TfiI 930 GATTC G^A[AT]TC
1883 GATTC
Tth111I 3119 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3990 GACGCAGTC
XmaCI 2661 CCCGGG C^CCGGG
2875 CCCGGG
XmaI 2661 CCCGGG C^CCGGG
2875 CCCGGG
XmnI 402 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1901 GAAGCGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AhdI AluI AlwI AlwNI ApaI
ApoI AspEI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrDI
BsrI BssKI BstEII BstF5I BstNI
BstPI BstXI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PaeR7I PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoI XhoII