##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AocI Aor51HI AscI AviII BcgI
BfrI BglII BlpI Bpu1102I BseAI
BsiWI BsmBI BsmI BspEI BspHI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I CelII CpoI Eco47III Eco81I
EcoRI EcoRV FseI FspI HindIII
HpaI MluI MroI NaeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1035 GGTACC G^GTACC
AccI 245 GTCTAC GT^[AC][GT]AC
AhdI 4050 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 326 GGGCCC GGGCC^C
Asp718I 1035 GGTACC G^GTACC
AspEI 4050 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 976 GGATCC G^GATCC
BbeI 784 GGCGCC GGCGC^C
BbrPI 455 CACGTG CAC^GTG
BbsI 4082 GAAGAC GAAGAC(2/6)
BciVI 1081 GTATCC GTATCC(6/5)
BclI 42 TGATCA T^GATCA
BpmI 1876 CTCCAG CTGGAG(16/14)
BpuAI 4082 GAAGAC GAAGAC(2/6)
BsaBI 3909 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2684 GAGACC GGTCTC(1/5)
BsaWI 234 ACCGGA [AT]^CCGG[AT]
BseRI 836 GAGGAG GAGGAG(10/8)
Bsp1407I 3508 TGTACA T^GTACA
BspDI 1928 ATCGAT AT^CGAT
BspMI 3705 GCAGGT ACCTGC(4/8)
BsrFI 1328 GCCGGT [GA]^CCGG[CT]
BsrGI 3508 TGTACA T^GTACA
Cfr10I 1328 GCCGGT [GA]^CCGG[CT]
ClaI 1928 ATCGAT AT^CGAT
EagI 95 CGGCCG C^GGCCG
Eam1105I 4050 GACTGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 95 CGGCCG C^GGCCG
Eco52I 95 CGGCCG C^GGCCG
EcoT22I 3230 ATGCAT ATGCA^T
FbaI 42 TGATCA T^GATCA
KasI 784 GGCGCC G^GCGCC
KpnI 1035 GGTACC GGTAC^C
KspI 11 CCGCGG CCGC^GG
MamI 3909 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1861 CAATTG C^AATTG
MunI 1861 CAATTG C^AATTG
NarI 784 GGCGCC GG^CGCC
NdeI 2941 CATATG CA^TATG
NheI 1547 GCTAGC G^CTAGC
NsiI 3230 ATGCAT ATGCA^T
PmaCI 455 CACGTG CAC^GTG
PmlI 455 CACGTG CAC^GTG
PstI 3920 CTGCAG CTGCA^G
PvuII 2717 CAGCTG CAG^CTG
SacII 11 CCGCGG CCGC^GG
SexAI 240 ACCTGGT A^CC[AT]GGT
SfiI 740 GGCCAGGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 784 GGCGCC GGC^GCC
SphI 2694 GCATGC GCATG^C
SspBI 3508 TGTACA T^GTACA
SspI 3563 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AcsI 2108 GAATTT [GA]^AATT[CT]
3456 GAATTT
AcyI 784 GGCGCC G[GA]^CG[CT]C
2062 GGCGTC
Alw44I 208 GTGCAC G^TGCAC
1431 GTGCAC
ApaLI 208 GTGCAC G^TGCAC
1431 GTGCAC
ApoI 2108 GAATTT [GA]^AATT[CT]
3456 GAATTT
AseI 2789 ATTAAT AT^TAAT
3560 ATTAAT
AsnI 2789 ATTAAT AT^TAAT
3560 ATTAAT
Asp700I 2388 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4082 GAAGACTTTC
AvrII 2333 CCTAGG C^CTAGG
3681 CCTAGG
BbvCI 382 CCTCAGC CCTCAGC(-5/-2)
2638 GCTGAGG
BglI 741 GCCAGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
750 GCCAAGGAGGC
BlnI 2333 CCTAGG C^CTAGG
3681 CCTAGG
BsaAI 455 CACGTG [CT]AC^GT[GA]
3251 TACGTG
BsaHI 784 GGCGCC G[GA]^CG[CT]C
2062 GGCGTC
BsiEI 95 CGGCCG CG[GA][CT]^CG
961 CGACCG
BsrBI 964 CCGCTC CCGCTC(-3/-3)
1528 CCGCTC
BsrDI 727 CATTGC GCAATG(2/0)
3632 CATTGC
BstXI 69 CCATGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
523 CCATGGTACTGG
BstYI 225 AGATCC [GA]^GATC[CT]
976 GGATCC
DraIII 690 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
3491 CACTTTGTG
DrdI 1353 GACATGTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1614 GACATCATGGTC
EarI 811 CTCTTC CTCTTC(1/4)
2006 GAAGAG
EcoNI 421 CCTCATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3744 CCTACTGAAGG
HaeII 784 GGCGCC [GA]GCGC^[CT]
1996 GGCGCT
HgaI 86 GCGTC GACGC(5/10)
2063 GCGTC
Hin1I 784 GGCGCC G[GA]^CG[CT]C
2062 GGCGTC
HincII 891 GTCAAC GT[CT]^[GA]AC
3714 GTTGAC
HindII 891 GTCAAC GT[CT]^[GA]AC
3714 GTTGAC
Ksp632I 811 CTCTTC CTCTTC(1/4)
2006 GAAGAG
MflI 225 AGATCC [GA]^GATC[CT]
976 GGATCC
PshBI 2789 ATTAAT AT^TAAT
3560 ATTAAT
SapI 2006 GAAGAGC GCTCTTC(1/4)
810 GCTCTTC
SmaI 1102 CCCGGG CCC^GGG
2674 CCCGGG
SmlI 1153 CTCAAG C^T[CT][GA]AG
1766 CTTGAG
StuI 403 AGGCCT AGG^CCT
3984 AGGCCT
TaqI 1929 TCGA T^CGA
3162 TCGA
TfiI 2247 GAATC G^A[AT]TC
2845 GAATC
TthHB8I 1929 TCGA T^CGA
3162 TCGA
XcmI 66 CCACCATGTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
726 CCATTGCCATGATGG
XhoII 225 AGATCC [GA]^GATC[CT]
976 GGATCC
XmaCI 1102 CCCGGG C^CCGGG
2674 CCCGGG
XmaI 1102 CCCGGG C^CCGGG
2674 CCCGGG
XmnI 2388 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4082 GAAGACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AspHI AspI AvaI
AvaII BalI BanI BanII BbvI
BcnI BfaI BmyI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspLU11I BsrI BssKI
BstAPI BstEII BstF5I BstNI BstPI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PleI PpuMI RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI StyI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I