##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BbeI BbrPI
BbvCI BciVI BlnI BsaAI BsiWI
BspHI BssSI Bst1107I BstEII BstPI
BstZ17I CpoI DraIII DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI FseI FspI HaeII
HpaI KasI KpnI KspI MluI
NaeI NarI NcoI NgoMIV NheI
NotI PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccIII 1007 TCCGGA T^CCGGA
AcyI 2098 GGCGTC G[GA]^CG[CT]C
AflII 1464 CTTAAG C^TTAAG
Alw44I 1104 GTGCAC G^TGCAC
AocI 2076 CCTCAGG CC^T[ACGT]AGG
ApaLI 1104 GTGCAC G^TGCAC
Asp700I 1586 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1104 GTGCAC G[AT]GC[AT]^C
AspI 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 648 TGGCCA TGG^CCA
BamHI 45 GGATCC G^GATCC
BcgI 2715 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1464 CTTAAG C^TTAAG
BsaBI 1628 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2098 GGCGTC G[GA]^CG[CT]C
BsaI 1773 GAGACC GGTCTC(1/5)
BseAI 1007 TCCGGA T^CCGGA
BsgI 306 CTGCAC GTGCAG(16/14)
BsiEI 1738 CGGTCG CG[GA][CT]^CG
BsiHKAI 1104 GTGCAC G[AT]GC[AT]^C
BsmBI 226 GAGACG CGTCTC(1/5)
BspDI 1836 ATCGAT AT^CGAT
BspEI 1007 TCCGGA T^CCGGA
BsrFI 2095 ACCGGC [GA]^CCGG[CT]
BssHII 1944 GCGCGC G^CGCGC
BstBI 192 TTCGAA TT^CGAA
BstXI 1054 CCACCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2076 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2095 ACCGGC [GA]^CCGG[CT]
ClaI 1836 ATCGAT AT^CGAT
DrdI 831 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2076 CCTCAGG CC^T[ACGT]AGG
EcoRV 1542 GATATC GAT^ATC
FauI 71 GCGGG CCCGC(4/6)
Hin1I 2098 GGCGTC G[GA]^CG[CT]C
HincII 1254 GTTGAC GT[CT]^[GA]AC
HindII 1254 GTTGAC GT[CT]^[GA]AC
HindIII 563 AAGCTT A^AGCTT
MamI 1628 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2383 CAATTG C^AATTG
MluNI 648 TGGCCA TGG^CCA
MroI 1007 TCCGGA T^CCGGA
MscI 648 TGGCCA TGG^CCA
MunI 2383 CAATTG C^AATTG
NruI 407 TCGCGA TCG^CGA
NspV 192 TTCGAA TT^CGAA
PflFI 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfuI 192 TTCGAA TT^CGAA
SmaI 1057 CCCGGG CCC^GGG
SmlI 1464 CTTAAG C^T[CT][GA]AG
SphI 3031 GCATGC GCATG^C
SspI 719 AATATT AAT^ATT
StuI 1021 AGGCCT AGG^CCT
Tth111I 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1057 CCCGGG C^CCGGG
XmaI 1057 CCCGGG C^CCGGG
XmnI 1586 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 610 GAATTT [GA]^AATT[CT]
1614 AAATTC
AlwNI 1899 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
2680 CAGTGACTG
ApoI 610 GAATTT [GA]^AATT[CT]
1614 AAATTC
AseI 716 ATTAAT AT^TAAT
2327 ATTAAT
AsnI 716 ATTAAT AT^TAAT
2327 ATTAAT
AvaI 142 CCCGAG C^[CT]CG[GA]G
1057 CCCGGG
BanI 1656 GGCACC G^G[CT][GA]CC
1800 GGTGCC
BclI 956 TGATCA T^GATCA
1171 TGATCA
BglI 1202 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2235 GCCAGCCAGGC
BglII 466 AGATCT A^GATCT
847 AGATCT
BpmI 1730 CTCCAG CTGGAG(16/14)
1791 CTCCAG
BsaWI 251 ACCGGA [AT]^CCGG[AT]
1007 TCCGGA
BsmI 313 GAATGC GAATGC(1/-1)
343 GCATTC
BsoBI 142 CCCGAG C^[CT]CG[GA]G
1057 CCCGGG
BspLU11I 599 ACATGT A^CATGT
2632 ACATGT
BsrBI 69 GAGCGG CCGCTC(-3/-3)
1497 GAGCGG
BstAPI 308 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3069 GCATTGTGTGC
DraI 740 TTTAAA TTT^AAA
2855 TTTAAA
DraII 61 AGGTCCC [GA]G^G[ACGT]CC[CT]
1648 GGGACCC
EaeI 648 TGGCCA [CT]^GGCC[GA]
2170 TGGCCG
EarI 2795 CTCTTC CTCTTC(1/4)
2394 GAAGAG
EcoO109I 61 AGGTCCC [GA]G^G[ACGT]CC[CT]
1648 GGGACCC
EcoT14I 1513 CCTTGG C^C[AT][AT]GG
2626 CCAAGG
EcoT22I 341 ATGCAT ATGCA^T
3029 ATGCAT
FbaI 956 TGATCA T^GATCA
1171 TGATCA
HgaI 162 GACGC GACGC(5/10)
2099 GCGTC
Ksp632I 2795 CTCTTC CTCTTC(1/4)
2394 GAAGAG
NsiI 341 ATGCAT ATGCA^T
3029 ATGCAT
PflMI 425 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1121 CCACAGCATGG
PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT]
1648 GGGACCC
PshAI 2094 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2727 GACTAGAGTC
PshBI 716 ATTAAT AT^TAAT
2327 ATTAAT
SexAI 1693 ACCTGGT A^CC[AT]GGT
2113 ACCTGGT
StyI 1513 CCTTGG C^C[AT][AT]GG
2626 CCAAGG
Van91I 425 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1121 CCACAGCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaII BanII BbsI BbvI
BcnI BfaI BlpI BmyI Bpu1102I
BpuAI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII