################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII Acc65I AccI AclI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BbvCI BciVI BlnI BsaAI BsiWI BspHI BssSI Bst1107I BstEII BstPI BstZ17I CpoI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI FseI FspI HaeII HpaI KasI KpnI KspI MluI NaeI NarI NcoI NgoMIV NheI NotI PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccIII 1007 TCCGGA T^CCGGA AcyI 2098 GGCGTC G[GA]^CG[CT]C AflII 1464 CTTAAG C^TTAAG Alw44I 1104 GTGCAC G^TGCAC AocI 2076 CCTCAGG CC^T[ACGT]AGG ApaLI 1104 GTGCAC G^TGCAC Asp700I 1586 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 1104 GTGCAC G[AT]GC[AT]^C AspI 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 648 TGGCCA TGG^CCA BamHI 45 GGATCC G^GATCC BcgI 2715 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1464 CTTAAG C^TTAAG BsaBI 1628 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2098 GGCGTC G[GA]^CG[CT]C BsaI 1773 GAGACC GGTCTC(1/5) BseAI 1007 TCCGGA T^CCGGA BsgI 306 CTGCAC GTGCAG(16/14) BsiEI 1738 CGGTCG CG[GA][CT]^CG BsiHKAI 1104 GTGCAC G[AT]GC[AT]^C BsmBI 226 GAGACG CGTCTC(1/5) BspDI 1836 ATCGAT AT^CGAT BspEI 1007 TCCGGA T^CCGGA BsrFI 2095 ACCGGC [GA]^CCGG[CT] BssHII 1944 GCGCGC G^CGCGC BstBI 192 TTCGAA TT^CGAA BstXI 1054 CCACCCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2076 CCTCAGG CC^T[ACGT]AGG Cfr10I 2095 ACCGGC [GA]^CCGG[CT] ClaI 1836 ATCGAT AT^CGAT DrdI 831 GACATGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2076 CCTCAGG CC^T[ACGT]AGG EcoRV 1542 GATATC GAT^ATC FauI 71 GCGGG CCCGC(4/6) Hin1I 2098 GGCGTC G[GA]^CG[CT]C HincII 1254 GTTGAC GT[CT]^[GA]AC HindII 1254 GTTGAC GT[CT]^[GA]AC HindIII 563 AAGCTT A^AGCTT MamI 1628 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2383 CAATTG C^AATTG MluNI 648 TGGCCA TGG^CCA MroI 1007 TCCGGA T^CCGGA MscI 648 TGGCCA TGG^CCA MunI 2383 CAATTG C^AATTG NruI 407 TCGCGA TCG^CGA NspV 192 TTCGAA TT^CGAA PflFI 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC SfuI 192 TTCGAA TT^CGAA SmaI 1057 CCCGGG CCC^GGG SmlI 1464 CTTAAG C^T[CT][GA]AG SphI 3031 GCATGC GCATG^C SspI 719 AATATT AAT^ATT StuI 1021 AGGCCT AGG^CCT Tth111I 1901 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1057 CCCGGG C^CCGGG XmaI 1057 CCCGGG C^CCGGG XmnI 1586 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcsI 610 GAATTT [GA]^AATT[CT] 1614 AAATTC AlwNI 1899 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 2680 CAGTGACTG ApoI 610 GAATTT [GA]^AATT[CT] 1614 AAATTC AseI 716 ATTAAT AT^TAAT 2327 ATTAAT AsnI 716 ATTAAT AT^TAAT 2327 ATTAAT AvaI 142 CCCGAG C^[CT]CG[GA]G 1057 CCCGGG BanI 1656 GGCACC G^G[CT][GA]CC 1800 GGTGCC BclI 956 TGATCA T^GATCA 1171 TGATCA BglI 1202 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2235 GCCAGCCAGGC BglII 466 AGATCT A^GATCT 847 AGATCT BpmI 1730 CTCCAG CTGGAG(16/14) 1791 CTCCAG BsaWI 251 ACCGGA [AT]^CCGG[AT] 1007 TCCGGA BsmI 313 GAATGC GAATGC(1/-1) 343 GCATTC BsoBI 142 CCCGAG C^[CT]CG[GA]G 1057 CCCGGG BspLU11I 599 ACATGT A^CATGT 2632 ACATGT BsrBI 69 GAGCGG CCGCTC(-3/-3) 1497 GAGCGG BstAPI 308 GCACAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3069 GCATTGTGTGC DraI 740 TTTAAA TTT^AAA 2855 TTTAAA DraII 61 AGGTCCC [GA]G^G[ACGT]CC[CT] 1648 GGGACCC EaeI 648 TGGCCA [CT]^GGCC[GA] 2170 TGGCCG EarI 2795 CTCTTC CTCTTC(1/4) 2394 GAAGAG EcoO109I 61 AGGTCCC [GA]G^G[ACGT]CC[CT] 1648 GGGACCC EcoT14I 1513 CCTTGG C^C[AT][AT]GG 2626 CCAAGG EcoT22I 341 ATGCAT ATGCA^T 3029 ATGCAT FbaI 956 TGATCA T^GATCA 1171 TGATCA HgaI 162 GACGC GACGC(5/10) 2099 GCGTC Ksp632I 2795 CTCTTC CTCTTC(1/4) 2394 GAAGAG NsiI 341 ATGCAT ATGCA^T 3029 ATGCAT PflMI 425 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1121 CCACAGCATGG PpuMI 61 AGGTCCC [GA]G^G[AT]CC[CT] 1648 GGGACCC PshAI 2094 GACCGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2727 GACTAGAGTC PshBI 716 ATTAAT AT^TAAT 2327 ATTAAT SexAI 1693 ACCTGGT A^CC[AT]GGT 2113 ACCTGGT StyI 1513 CCTTGG C^C[AT][AT]GG 2626 CCAAGG Van91I 425 CCAAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1121 CCACAGCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AvaII BanII BbsI BbvI BcnI BfaI BlpI BmyI Bpu1102I BpuAI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII