##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AflII AflIII AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AvaI BanI BbeI
BbrPI BbvCI BciVI BfrI BglI
BlpI Bpu1102I BsaAI BsiWI BsoBI
BspLU11I BssHII Bst1107I BstBI BstZ17I
CelII CpoI DraII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO109I
EcoRV FseI HaeII HindIII HpaI
KasI KpnI KspI MfeI MluI
MunI NaeI NarI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
RsrII SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 2277 GACGTC GACGT^C
AccII 2285 CGCG CG^CG
AclI 2445 AACGTT AA^CGTT
Alw44I 3758 GTGCAC G^TGCAC
ApaLI 3758 GTGCAC G^TGCAC
Asp700I 2991 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3547 TGCGCA TGC^GCA
AvrII 3180 CCTAGG C^CTAGG
BclI 1099 TGATCA T^GATCA
BglII 1456 AGATCT A^GATCT
BlnI 3180 CCTAGG C^CTAGG
BsiEI 1557 CGATCG CG[GA][CT]^CG
BspMI 2710 ACCTGC ACCTGC(4/8)
BsrBI 3649 CCGCTC CCGCTC(-3/-3)
BsrFI 1393 ACCGGC [GA]^CCGG[CT]
BstEII 1053 GGTGACC G^GT[ACGT]ACC
BstPI 1053 GGTGACC G^GT[ACGT]ACC
BstUI 2285 CGCG CG^CG
Cfr10I 1393 ACCGGC [GA]^CCGG[CT]
DraIII 4222 CACTAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1339 GAAGAG CTCTTC(1/4)
EcoO65I 1053 GGTGACC G^GT[ACGT]ACC
EcoRI 4402 GAATTC G^AATTC
FauI 3648 CCCGC CCCGC(4/6)
FbaI 1099 TGATCA T^GATCA
FspI 3547 TGCGCA TGC^GCA
HgaI 3408 GACGC GACGC(5/10)
Ksp632I 1339 GAAGAG CTCTTC(1/4)
MvnI 2285 CGCG CG^CG
NdeI 4150 CATATG CA^TATG
NheI 3558 GCTAGC G^CTAGC
Psp1406I 2445 AACGTT AA^CGTT
PvuI 1557 CGATCG CGAT^CG
ScaI 585 AGTACT AGT^ACT
SexAI 340 ACCAGGT A^CC[AT]GGT
XmnI 2991 GAACACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccIII 2184 TCCGGA T^CCGGA
3427 TCCGGA
AcyI 2277 GACGTC G[GA]^CG[CT]C
3408 GACGCC
AspI 1344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3386 GACAAAGTC
BamHI 2087 GGATCC G^GATCC
4959 GGATCC
BsaHI 2277 GACGTC G[GA]^CG[CT]C
3408 GACGCC
BseAI 2184 TCCGGA T^CCGGA
3427 TCCGGA
BsgI 161 GTGCAG GTGCAG(16/14)
277 GTGCAG
BsmBI 2279 CGTCTC CGTCTC(1/5)
4634 GAGACG
BsmI 905 GAATGC GAATGC(1/-1)
76 GCATTC
Bsp1407I 640 TGTACA T^GTACA
703 TGTACA
BspDI 870 ATCGAT AT^CGAT
1559 ATCGAT
BspEI 2184 TCCGGA T^CCGGA
3427 TCCGGA
BspHI 817 TCATGA T^CATGA
1325 TCATGA
BsrGI 640 TGTACA T^GTACA
703 TGTACA
BstAPI 2835 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4149 GCATATGTTGC
ClaI 870 ATCGAT AT^CGAT
1559 ATCGAT
DraI 3984 TTTAAA TTT^AAA
4338 TTTAAA
EcoNI 203 CCTAGTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4041 CCTTTCCCAGG
EcoT22I 3195 ATGCAT ATGCA^T
4016 ATGCAT
Hin1I 2277 GACGTC G[GA]^CG[CT]C
3408 GACGCC
HincII 1743 GTTGAC GT[CT]^[GA]AC
2944 GTCAAC
HindII 1743 GTTGAC GT[CT]^[GA]AC
2944 GTCAAC
MroI 2184 TCCGGA T^CCGGA
3427 TCCGGA
MslI 1926 CACAAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4408 CATGAAGATG
NcoI 515 CCATGG C^CATGG
2762 CCATGG
NsiI 3195 ATGCAT ATGCA^T
4016 ATGCAT
PflFI 1344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3386 GACAAAGTC
RcaI 817 TCATGA T^CATGA
1325 TCATGA
SacI 684 GAGCTC GAGCT^C
4233 GAGCTC
SspBI 640 TGTACA T^GTACA
703 TGTACA
StuI 1484 AGGCCT AGG^CCT
2542 AGGCCT
Tth111I 1344 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3386 GACAAAGTC
XbaI 3528 TCTAGA T^CTAGA
3910 TCTAGA
XcmI 891 CCACTGCCCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3616 CCAGAGGAGGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaI BsaJI BsaWI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI Eco81I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PshAI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII