##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
Acc65I AclI AgeI AocI AscI
Asp718I AspI AviII AvrII BciVI
BglII BlnI BsiWI BsmBI BsmI
Bsp1407I BspDI BspLU11I BsrGI BssHII
BstBI Bsu36I ClaI CpoI EagI
EclXI Eco52I Eco81I EcoRV EcoT22I
FseI FspI HindIII HpaI KpnI
KspI MfeI MluI MunI NdeI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII ScaI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
Tth111I
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 1902 GACGTC GACGT^C
AccII 3357 CGCG CG^CG
AccIII 978 TCCGGA T^CCGGA
AcsI 58 GAATTC [GA]^AATT[CT]
AflIII 745 ACGTGT A^C[GA][CT]GT
Aor51HI 1858 AGCGCT AGC^GCT
ApoI 58 GAATTC [GA]^AATT[CT]
AseI 1770 ATTAAT AT^TAAT
AsnI 1770 ATTAAT AT^TAAT
Asp700I 185 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 514 GGCGCC GGCGC^C
BbrPI 3036 CACGTG CAC^GTG
BcgI 2546 CGAGAGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2899 TGATCA T^GATCA
BlpI 2564 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2564 GCTAAGC GC^T[ACGT]AGC
BsaWI 978 TCCGGA [AT]^CCGG[AT]
BseAI 978 TCCGGA T^CCGGA
BsiEI 461 CGACCG CG[GA][CT]^CG
BspEI 978 TCCGGA T^CCGGA
BspHI 193 TCATGA T^CATGA
BssSI 3885 CACGAG CACGAG(-5/-1)
Bst1107I 4055 GTATAC GTA^TAC
BstAPI 1098 GCACCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2 GGTCACC G^GT[ACGT]ACC
BstPI 2 GGTCACC G^GT[ACGT]ACC
BstUI 3357 CGCG CG^CG
BstZ17I 4055 GTATAC GTA^TAC
CelII 2564 GCTAAGC GC^T[ACGT]AGC
DraI 4233 TTTAAA TTT^AAA
Eco47III 1858 AGCGCT AGC^GCT
EcoO65I 2 GGTCACC G^GT[ACGT]ACC
EcoRI 58 GAATTC G^AATTC
FbaI 2899 TGATCA T^GATCA
HincII 1899 GTCGAC GT[CT]^[GA]AC
HindII 1899 GTCGAC GT[CT]^[GA]AC
KasI 514 GGCGCC G^GCGCC
MroI 978 TCCGGA T^CCGGA
MvnI 3357 CGCG CG^CG
NarI 514 GGCGCC GG^CGCC
NheI 942 GCTAGC G^CTAGC
PmaCI 3036 CACGTG CAC^GTG
PmlI 3036 CACGTG CAC^GTG
PshAI 3970 GACATCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1770 ATTAAT AT^TAAT
RcaI 193 TCATGA T^CATGA
SalI 1899 GTCGAC G^TCGAC
SapI 1201 GCTCTTC GCTCTTC(1/4)
SbfI 2423 CCTGCAGG CCTGCA^GG
SexAI 3046 ACCAGGT A^CC[AT]GGT
SfiI 3997 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 514 GGCGCC GGC^GCC
Sse8387I 2423 CCTGCAGG CCTGCA^GG
StuI 3649 AGGCCT AGG^CCT
XbaI 1419 TCTAGA T^CTAGA
XmnI 185 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccI 1899 GTCGAC GT^[AC][GT]AC
4055 GTATAC
AflII 2841 CTTAAG C^TTAAG
3240 CTTAAG
AhdI 2913 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3985 GACTCCAGGTC
Alw44I 2385 GTGCAC G^TGCAC
2488 GTGCAC
ApaLI 2385 GTGCAC G^TGCAC
2488 GTGCAC
AspEI 2913 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3985 GACTCCAGGTC
BbvCI 1814 GCTGAGG CCTCAGC(-5/-2)
2892 GCTGAGG
BfrI 2841 CTTAAG C^TTAAG
3240 CTTAAG
BglI 3104 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3998 GCCTGCATGGC
BsaAI 744 TACGTG [CT]AC^GT[GA]
3036 CACGTG
BsaBI 3863 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3869 GATCTACATC
BsrDI 2552 CATTGC GCAATG(2/0)
2999 CATTGC
BstXI 1390 CCATCGTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2777 CCACCTGCGTGG
DraIII 2661 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
3036 CACGTGGTG
Eam1105I 2913 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3985 GACTCCAGGTC
MamI 3863 GATCCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3869 GATCTACATC
NaeI 1073 GCCGGC GCC^GGC
1319 GCCGGC
NcoI 3664 CCATGG C^CATGG
4050 CCATGG
NgoMIV 1073 GCCGGC G^CCGGC
1319 GCCGGC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PaeR7I PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoI XhoII
XmaCI XmaI