##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AccII AclI AcsI AflII AgeI
Aor51HI ApoI AscI AseI AsnI
Asp700I AvrII BbeI BbrPI BciVI
BclI BfrI BlnI BsaAI BsiEI
BsiWI BsmI Bsp1407I BsrGI BssHII
BssSI Bst1107I BstUI BstZ17I CpoI
DraI DraIII EagI EclXI Eco47III
Eco52I EcoRI EcoT22I FbaI FseI
HincII HindII HindIII HpaI KasI
KspI MfeI MluI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SwaI XbaI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1576 GACGTC GACGT^C
Acc65I 1106 GGTACC G^GTACC
AccI 762 GTCTAC GT^[AC][GT]AC
AccIII 3731 TCCGGA T^CCGGA
AflIII 758 ACATGT A^C[GA][CT]GT
AhdI 33 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1106 GGTACC G^GTACC
AspEI 33 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3523 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1553 TGCGCA TGC^GCA
BsaWI 3731 TCCGGA [AT]^CCGG[AT]
BseAI 3731 TCCGGA T^CCGGA
BspDI 1652 ATCGAT AT^CGAT
BspEI 3731 TCCGGA T^CCGGA
BspLU11I 758 ACATGT A^CATGT
BstAPI 1665 GCAGCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 812 TTCGAA TT^CGAA
BstEII 1315 GGTTACC G^GT[ACGT]ACC
BstPI 1315 GGTTACC G^GT[ACGT]ACC
BstXI 3233 CCAGATTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 1652 ATCGAT AT^CGAT
Eam1105I 33 GACCAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1315 GGTTACC G^GT[ACGT]ACC
EcoRV 1624 GATATC GAT^ATC
FspI 1553 TGCGCA TGC^GCA
HaeII 3160 GGCGCT [GA]GCGC^[CT]
KpnI 1106 GGTACC GGTAC^C
MaeII 1577 ACGT A^CGT
MroI 3731 TCCGGA T^CCGGA
NspI 758 ACATGT [GA]CATG^[CT]
NspV 812 TTCGAA TT^CGAA
PflFI 3523 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SfuI 812 TTCGAA TT^CGAA
SspI 710 AATATT AAT^ATT
StuI 17 AGGCCT AGG^CCT
Tth111I 3523 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcyI 73 GGCGTC G[GA]^CG[CT]C
1576 GACGTC
BcgI 2656 CGACACCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1644 GCAGATCCATCG
BglI 2296 GCCAATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2627 GCCACCATGGC
BglII 1814 AGATCT A^GATCT
2568 AGATCT
BlpI 3220 GCTGAGC GC^T[ACGT]AGC
4346 GCTGAGC
Bpu1102I 3220 GCTGAGC GC^T[ACGT]AGC
4346 GCTGAGC
BsaBI 1825 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2482 GATGCTTATC
BsaHI 73 GGCGTC G[GA]^CG[CT]C
1576 GACGTC
BsmBI 1578 CGTCTC CGTCTC(1/5)
4283 GAGACG
BsrBI 642 CCGCTC CCGCTC(-3/-3)
2284 CCGCTC
BsrFI 269 ACCGGC [GA]^CCGG[CT]
1495 GCCGGT
CelII 3220 GCTGAGC GC^T[ACGT]AGC
4346 GCTGAGC
Cfr10I 269 ACCGGC [GA]^CCGG[CT]
1495 GCCGGT
Hin1I 73 GGCGTC G[GA]^CG[CT]C
1576 GACGTC
MamI 1825 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2482 GATGCTTATC
NdeI 1130 CATATG CA^TATG
2972 CATATG
PshAI 69 GACAGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
347 GACCCGAGTC
ScaI 1347 AGTACT AGT^ACT
3455 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AciI AfaI AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PaeR7I PflMI PleI PpuMI
PstI PvuII RcaI RsaI SacI
SapI Sau3AI Sau96I ScrFI SexAI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoI
XhoII