################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AclI AscI Asp718I AspI AviII BbrPI BclI BglII BsaBI BseAI BsiEI BsiWI BspDI BspEI BspHI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I EcoT22I FbaI FseI FspI HgaI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 3699 GTAGAC GT^[AC][GT]AC AccII 3949 CGCG CG^CG AflII 3997 CTTAAG C^TTAAG AflIII 4488 ACATGT A^C[GA][CT]GT AgeI 3490 ACCGGT A^CCGGT Alw44I 541 GTGCAC G^TGCAC Aor51HI 3281 AGCGCT AGC^GCT ApaLI 541 GTGCAC G^TGCAC AseI 4115 ATTAAT AT^TAAT AsnI 4115 ATTAAT AT^TAAT Asp700I 3427 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3301 CCTAGG C^CTAGG BcgI 2931 GCAGCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4760 GTATCC GTATCC(6/5) BfrI 3997 CTTAAG C^TTAAG BlnI 3301 CCTAGG C^CTAGG BsaAI 4064 CACGTA [CT]AC^GT[GA] BsaWI 3490 ACCGGT [AT]^CCGG[AT] BsmBI 3668 GAGACG CGTCTC(1/5) Bsp1407I 3255 TGTACA T^GTACA BspLU11I 4488 ACATGT A^CATGT BsrFI 3490 ACCGGT [GA]^CCGG[CT] BsrGI 3255 TGTACA T^GTACA BstUI 3949 CGCG CG^CG Cfr10I 3490 ACCGGT [GA]^CCGG[CT] DraIII 3946 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 3281 AGCGCT AGC^GCT EcoRI 3976 GAATTC G^AATTC EcoRV 36 GATATC GAT^ATC HincII 1693 GTCAAC GT[CT]^[GA]AC HindII 1693 GTCAAC GT[CT]^[GA]AC MvnI 3949 CGCG CG^CG PinAI 3490 ACCGGT A^CCGGT PshBI 4115 ATTAAT AT^TAAT SphI 1440 GCATGC GCATG^C SspBI 3255 TGTACA T^GTACA SspI 4283 AATATT AAT^ATT StuI 4754 AGGCCT AGG^CCT XbaI 4483 TCTAGA T^CTAGA XmnI 3427 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 363 GGCGCC G[GA]^CG[CT]C 2400 GGCGCC AvaI 2684 CCCGGG C^[CT]CG[GA]G 3754 CCCGGG BamHI 2958 GGATCC G^GATCC 3351 GGATCC BbeI 363 GGCGCC GGCGC^C 2400 GGCGCC BglI 877 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2313 GCCCCATCGGC BlpI 1382 GCTGAGC GC^T[ACGT]AGC 2581 GCTCAGC Bpu1102I 1382 GCTGAGC GC^T[ACGT]AGC 2581 GCTCAGC BsaHI 363 GGCGCC G[GA]^CG[CT]C 2400 GGCGCC BsoBI 2684 CCCGGG C^[CT]CG[GA]G 3754 CCCGGG BsrDI 570 GCAATG GCAATG(2/0) 976 GCAATG BstXI 1233 CCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1308 CCACCTTCCTGG CelII 1382 GCTGAGC GC^T[ACGT]AGC 2581 GCTCAGC EcoNI 2892 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3689 CCTGGGGCAGG Hin1I 363 GGCGCC G[GA]^CG[CT]C 2400 GGCGCC HindIII 3399 AAGCTT A^AGCTT 4355 AAGCTT KasI 363 GGCGCC G^GCGCC 2400 GGCGCC NarI 363 GGCGCC GG^CGCC 2400 GGCGCC PpuMI 2679 GGGACCC [GA]G^G[AT]CC[CT] 4629 AGGTCCC ScaI 1856 AGTACT AGT^ACT 3202 AGTACT SfoI 363 GGCGCC GGC^GCC 2400 GGCGCC SmaI 2684 CCCGGG CCC^GGG 3754 CCCGGG XmaCI 2684 CCCGGG C^CCGGG 3754 CCCGGG XmaI 2684 CCCGGG C^CCGGG 3754 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 117 enzymes) # ######################################### AciI AcsI AfaI AhdI AluI AlwI AlwNI AocI ApaI ApoI AspEI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrI BssKI BstAPI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII