##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AscI
Asp718I AspI AviII BbrPI BclI
BglII BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspHI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DrdI EagI EclXI
Eco52I EcoO65I EcoT22I FbaI FseI
FspI HgaI HpaI KpnI KspI
MamI MfeI MluI MroI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 3699 GTAGAC GT^[AC][GT]AC
AccII 3949 CGCG CG^CG
AflII 3997 CTTAAG C^TTAAG
AflIII 4488 ACATGT A^C[GA][CT]GT
AgeI 3490 ACCGGT A^CCGGT
Alw44I 541 GTGCAC G^TGCAC
Aor51HI 3281 AGCGCT AGC^GCT
ApaLI 541 GTGCAC G^TGCAC
AseI 4115 ATTAAT AT^TAAT
AsnI 4115 ATTAAT AT^TAAT
Asp700I 3427 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3301 CCTAGG C^CTAGG
BcgI 2931 GCAGCTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4760 GTATCC GTATCC(6/5)
BfrI 3997 CTTAAG C^TTAAG
BlnI 3301 CCTAGG C^CTAGG
BsaAI 4064 CACGTA [CT]AC^GT[GA]
BsaWI 3490 ACCGGT [AT]^CCGG[AT]
BsmBI 3668 GAGACG CGTCTC(1/5)
Bsp1407I 3255 TGTACA T^GTACA
BspLU11I 4488 ACATGT A^CATGT
BsrFI 3490 ACCGGT [GA]^CCGG[CT]
BsrGI 3255 TGTACA T^GTACA
BstUI 3949 CGCG CG^CG
Cfr10I 3490 ACCGGT [GA]^CCGG[CT]
DraIII 3946 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 3281 AGCGCT AGC^GCT
EcoRI 3976 GAATTC G^AATTC
EcoRV 36 GATATC GAT^ATC
HincII 1693 GTCAAC GT[CT]^[GA]AC
HindII 1693 GTCAAC GT[CT]^[GA]AC
MvnI 3949 CGCG CG^CG
PinAI 3490 ACCGGT A^CCGGT
PshBI 4115 ATTAAT AT^TAAT
SphI 1440 GCATGC GCATG^C
SspBI 3255 TGTACA T^GTACA
SspI 4283 AATATT AAT^ATT
StuI 4754 AGGCCT AGG^CCT
XbaI 4483 TCTAGA T^CTAGA
XmnI 3427 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 363 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
AvaI 2684 CCCGGG C^[CT]CG[GA]G
3754 CCCGGG
BamHI 2958 GGATCC G^GATCC
3351 GGATCC
BbeI 363 GGCGCC GGCGC^C
2400 GGCGCC
BglI 877 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2313 GCCCCATCGGC
BlpI 1382 GCTGAGC GC^T[ACGT]AGC
2581 GCTCAGC
Bpu1102I 1382 GCTGAGC GC^T[ACGT]AGC
2581 GCTCAGC
BsaHI 363 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
BsoBI 2684 CCCGGG C^[CT]CG[GA]G
3754 CCCGGG
BsrDI 570 GCAATG GCAATG(2/0)
976 GCAATG
BstXI 1233 CCAGCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1308 CCACCTTCCTGG
CelII 1382 GCTGAGC GC^T[ACGT]AGC
2581 GCTCAGC
EcoNI 2892 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3689 CCTGGGGCAGG
Hin1I 363 GGCGCC G[GA]^CG[CT]C
2400 GGCGCC
HindIII 3399 AAGCTT A^AGCTT
4355 AAGCTT
KasI 363 GGCGCC G^GCGCC
2400 GGCGCC
NarI 363 GGCGCC GG^CGCC
2400 GGCGCC
PpuMI 2679 GGGACCC [GA]G^G[AT]CC[CT]
4629 AGGTCCC
ScaI 1856 AGTACT AGT^ACT
3202 AGTACT
SfoI 363 GGCGCC GGC^GCC
2400 GGCGCC
SmaI 2684 CCCGGG CCC^GGG
3754 CCCGGG
XmaCI 2684 CCCGGG C^CCGGG
3754 CCCGGG
XmaI 2684 CCCGGG C^CCGGG
3754 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI AocI ApaI ApoI
AspEI AspHI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrBI BsrI BssKI BstAPI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII