##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AviII AvrII BbeI BciVI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
Bst1107I BstBI BstZ17I CelII ClaI
DraI Eam1105I EcoT22I FseI FspI
HindIII HpaI KasI MamI MfeI
MluI MroI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 854 GGTACC G^GTACC
AccI 1119 GTCTAC GT^[AC][GT]AC
AflIII 4051 ACGTGT A^C[GA][CT]GT
Alw44I 693 GTGCAC G^TGCAC
ApaLI 693 GTGCAC G^TGCAC
Asp718I 854 GGTACC G^GTACC
AspI 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3076 GGATCC G^GATCC
BbrPI 3039 CACGTG CAC^GTG
BglI 3755 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 712 AGATCT A^GATCT
BspHI 2812 TCATGA T^CATGA
BsrBI 3734 CCGCTC CCGCTC(-3/-3)
BstEII 2189 GGTGACC G^GT[ACGT]ACC
BstPI 2189 GGTGACC G^GT[ACGT]ACC
CpoI 1966 CGGACCG CG^G[AT]CCG
DraIII 957 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4188 GACCGGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4350 CGGCCG C^GGCCG
EclXI 4350 CGGCCG C^GGCCG
Eco52I 4350 CGGCCG C^GGCCG
EcoO65I 2189 GGTGACC G^GT[ACGT]ACC
EcoRI 336 GAATTC G^AATTC
EcoRV 1870 GATATC GAT^ATC
KpnI 854 GGTACC GGTAC^C
KspI 3481 CCGCGG CCGC^GG
PflFI 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3039 CACGTG CAC^GTG
PmlI 3039 CACGTG CAC^GTG
RcaI 2812 TCATGA T^CATGA
RsrII 1966 CGGACCG CG^G[AT]CCG
SacII 3481 CCGCGG CCGC^GG
SphI 1739 GCATGC GCATG^C
Tth111I 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 297 CCTGAGG CC^T[ACGT]AGG
3026 CCTCAGG
BanI 854 GGTACC G^G[CT][GA]CC
1179 GGTGCC
BcgI 3047 CGAGCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3050 GCAGTTTGCTCG
BclI 2785 TGATCA T^GATCA
3374 TGATCA
BsaAI 849 TACGTG [CT]AC^GT[GA]
3039 CACGTG
BsiEI 4346 CGACCG CG[GA][CT]^CG
4350 CGGCCG
BsrDI 634 GCAATG GCAATG(2/0)
2982 GCAATG
BssSI 1509 CACGAG CACGAG(-5/-1)
2962 CACGAG
BstAPI 377 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2512 GCAAGACCTGC
Bsu36I 297 CCTGAGG CC^T[ACGT]AGG
3026 CCTCAGG
Eco81I 297 CCTGAGG CC^T[ACGT]AGG
3026 CCTCAGG
EcoNI 983 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4316 CCTGGTCAAGG
FbaI 2785 TGATCA T^GATCA
3374 TGATCA
HincII 1038 GTTGAC GT[CT]^[GA]AC
4185 GTTGAC
HindII 1038 GTTGAC GT[CT]^[GA]AC
4185 GTTGAC
MslI 1787 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4164 CACGGGGATG
NaeI 322 GCCGGC GCC^GGC
2341 GCCGGC
NcoI 349 CCATGG C^CATGG
1516 CCATGG
NgoMIV 322 GCCGGC G^CCGGC
2341 GCCGGC
SapI 3564 GAAGAGC GCTCTTC(1/4)
200 GCTCTTC
XcmI 1765 CCACCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3425 CCATGAGGACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI AspHI AvaI AvaII BalI
BanII BbsI BbvCI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco47III EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SmaI SmlI
Sse8387I StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmaCI XmaI