################################## # NO CUT ENZYMES ( 79 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI DraI Eam1105I EcoT22I FseI FspI HindIII HpaI KasI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SspI StuI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### Acc65I 854 GGTACC G^GTACC AccI 1119 GTCTAC GT^[AC][GT]AC AflIII 4051 ACGTGT A^C[GA][CT]GT Alw44I 693 GTGCAC G^TGCAC ApaLI 693 GTGCAC G^TGCAC Asp718I 854 GGTACC G^GTACC AspI 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 3076 GGATCC G^GATCC BbrPI 3039 CACGTG CAC^GTG BglI 3755 GCCACAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 712 AGATCT A^GATCT BspHI 2812 TCATGA T^CATGA BsrBI 3734 CCGCTC CCGCTC(-3/-3) BstEII 2189 GGTGACC G^GT[ACGT]ACC BstPI 2189 GGTGACC G^GT[ACGT]ACC CpoI 1966 CGGACCG CG^G[AT]CCG DraIII 957 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4188 GACCGGCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 4350 CGGCCG C^GGCCG EclXI 4350 CGGCCG C^GGCCG Eco52I 4350 CGGCCG C^GGCCG EcoO65I 2189 GGTGACC G^GT[ACGT]ACC EcoRI 336 GAATTC G^AATTC EcoRV 1870 GATATC GAT^ATC KpnI 854 GGTACC GGTAC^C KspI 3481 CCGCGG CCGC^GG PflFI 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3039 CACGTG CAC^GTG PmlI 3039 CACGTG CAC^GTG RcaI 2812 TCATGA T^CATGA RsrII 1966 CGGACCG CG^G[AT]CCG SacII 3481 CCGCGG CCGC^GG SphI 1739 GCATGC GCATG^C Tth111I 2873 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AocI 297 CCTGAGG CC^T[ACGT]AGG 3026 CCTCAGG BanI 854 GGTACC G^G[CT][GA]CC 1179 GGTGCC BcgI 3047 CGAGCAGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 3050 GCAGTTTGCTCG BclI 2785 TGATCA T^GATCA 3374 TGATCA BsaAI 849 TACGTG [CT]AC^GT[GA] 3039 CACGTG BsiEI 4346 CGACCG CG[GA][CT]^CG 4350 CGGCCG BsrDI 634 GCAATG GCAATG(2/0) 2982 GCAATG BssSI 1509 CACGAG CACGAG(-5/-1) 2962 CACGAG BstAPI 377 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2512 GCAAGACCTGC Bsu36I 297 CCTGAGG CC^T[ACGT]AGG 3026 CCTCAGG Eco81I 297 CCTGAGG CC^T[ACGT]AGG 3026 CCTCAGG EcoNI 983 CCTGGAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4316 CCTGGTCAAGG FbaI 2785 TGATCA T^GATCA 3374 TGATCA HincII 1038 GTTGAC GT[CT]^[GA]AC 4185 GTTGAC HindII 1038 GTTGAC GT[CT]^[GA]AC 4185 GTTGAC MslI 1787 CATCCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4164 CACGGGGATG NaeI 322 GCCGGC GCC^GGC 2341 GCCGGC NcoI 349 CCATGG C^CATGG 1516 CCATGG NgoMIV 322 GCCGGC G^CCGGC 2341 GCCGGC SapI 3564 GAAGAGC GCTCTTC(1/4) 200 GCTCTTC XcmI 1765 CCACCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 3425 CCATGAGGACAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmaI SmlI Sse8387I StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI