##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
AccI AccIII AclI AflIII AgeI
Aor51HI AscI AspI AviII AvrII
BamHI BbrPI BciVI BclI BlnI
BsaAI BsaBI BseAI BsiEI BsiWI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BssHII BssSI Bst1107I BstBI
BstZ17I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KspI MamI MfeI MluI
MroI MunI NaeI NgoMIV NotI
NruI NsiI NspV PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 3039 GACGTC GACGT^C
Acc65I 1682 GGTACC G^GTACC
AccII 1322 CGCG CG^CG
AflII 1826 CTTAAG C^TTAAG
AhdI 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2732 CCTGAGG CC^T[ACGT]AGG
ApaI 2913 GGGCCC GGGCC^C
Asp700I 339 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1682 GGTACC G^GTACC
AspEI 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 2427 GGCGCC GGCGC^C
BcgI 485 GCAGATCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1826 CTTAAG C^TTAAG
BsaI 2295 GAGACC GGTCTC(1/5)
BsaWI 151 TCCGGT [AT]^CCGG[AT]
BsmBI 2110 CGTCTC CGTCTC(1/5)
BstEII 1808 GGTCACC G^GT[ACGT]ACC
BstPI 1808 GGTCACC G^GT[ACGT]ACC
BstUI 1322 CGCG CG^CG
Bsu36I 2732 CCTGAGG CC^T[ACGT]AGG
Eam1105I 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2732 CCTGAGG CC^T[ACGT]AGG
EcoNI 1424 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1808 GGTCACC G^GT[ACGT]ACC
EcoRI 3373 GAATTC G^AATTC
HincII 2585 GTTGAC GT[CT]^[GA]AC
HindII 2585 GTTGAC GT[CT]^[GA]AC
KasI 2427 GGCGCC G^GCGCC
KpnI 1682 GGTACC GGTAC^C
MvnI 1322 CGCG CG^CG
NarI 2427 GGCGCC GG^CGCC
NdeI 312 CATATG CA^TATG
PacI 3361 TTAATTAA TTAAT^TAA
PaeR7I 2938 CTCGAG C^TCGAG
PshAI 1253 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2661 GAGCTC GAGCT^C
ScaI 2072 AGTACT AGT^ACT
SexAI 2332 ACCTGGT A^CC[AT]GGT
SfoI 2427 GGCGCC GGC^GCC
SgrAI 989 CGCCGGTG C[GA]^CCGG[CT]G
StuI 2682 AGGCCT AGG^CCT
TaqI 2939 TCGA T^CGA
TthHB8I 2939 TCGA T^CGA
XhoI 2938 CTCGAG C^TCGAG
XmnI 339 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AseI 3360 ATTAAT AT^TAAT
3364 ATTAAT
AsnI 3360 ATTAAT AT^TAAT
3364 ATTAAT
BalI 1598 TGGCCA TGG^CCA
1606 TGGCCA
BbsI 474 GAAGAC GAAGAC(2/6)
2975 GTCTTC
BglII 2353 AGATCT A^GATCT
2715 AGATCT
BlpI 639 GCTCAGC GC^T[ACGT]AGC
2028 GCTCAGC
Bpu1102I 639 GCTCAGC GC^T[ACGT]AGC
2028 GCTCAGC
BpuAI 474 GAAGAC GAAGAC(2/6)
2975 GTCTTC
BsrFI 990 GCCGGT [GA]^CCGG[CT]
1200 ACCGGC
BstXI 1384 CCACGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1723 CCACCCAGGTGG
BstYI 2353 AGATCT [GA]^GATC[CT]
2715 AGATCT
CelII 639 GCTCAGC GC^T[ACGT]AGC
2028 GCTCAGC
Cfr10I 990 GCCGGT [GA]^CCGG[CT]
1200 ACCGGC
HaeII 2241 GGCGCT [GA]GCGC^[CT]
2427 GGCGCC
MflI 2353 AGATCT [GA]^GATC[CT]
2715 AGATCT
MluNI 1598 TGGCCA TGG^CCA
1606 TGGCCA
MscI 1598 TGGCCA TGG^CCA
1606 TGGCCA
NheI 2190 GCTAGC G^CTAGC
3045 GCTAGC
PpuMI 996 GGGACCC [GA]G^G[AT]CC[CT]
1452 AGGTCCT
PshBI 3360 ATTAAT AT^TAAT
3364 ATTAAT
SapI 339 GAAGAGC GCTCTTC(1/4)
2376 GAAGAGC
SphI 1004 GCATGC GCATG^C
1331 GCATGC
SspI 3253 AATATT AAT^ATT
3456 AATATT
XcmI 562 CCATGGCCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1517 CCAGGGCTACGATGG
XhoII 2353 AGATCT [GA]^GATC[CT]
2715 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I