################################## # NO CUT ENZYMES ( 81 enzymes) # ################################## AccI AccIII AclI AflIII AgeI Aor51HI AscI AspI AviII AvrII BamHI BbrPI BciVI BclI BlnI BsaAI BsaBI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KspI MamI MfeI MluI MroI MunI NaeI NgoMIV NotI NruI NsiI NspV PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SalI SbfI SfiI SfuI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 3039 GACGTC GACGT^C Acc65I 1682 GGTACC G^GTACC AccII 1322 CGCG CG^CG AflII 1826 CTTAAG C^TTAAG AhdI 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2732 CCTGAGG CC^T[ACGT]AGG ApaI 2913 GGGCCC GGGCC^C Asp700I 339 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1682 GGTACC G^GTACC AspEI 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbeI 2427 GGCGCC GGCGC^C BcgI 485 GCAGATCAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 1826 CTTAAG C^TTAAG BsaI 2295 GAGACC GGTCTC(1/5) BsaWI 151 TCCGGT [AT]^CCGG[AT] BsmBI 2110 CGTCTC CGTCTC(1/5) BstEII 1808 GGTCACC G^GT[ACGT]ACC BstPI 1808 GGTCACC G^GT[ACGT]ACC BstUI 1322 CGCG CG^CG Bsu36I 2732 CCTGAGG CC^T[ACGT]AGG Eam1105I 1152 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2732 CCTGAGG CC^T[ACGT]AGG EcoNI 1424 CCTGGCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1808 GGTCACC G^GT[ACGT]ACC EcoRI 3373 GAATTC G^AATTC HincII 2585 GTTGAC GT[CT]^[GA]AC HindII 2585 GTTGAC GT[CT]^[GA]AC KasI 2427 GGCGCC G^GCGCC KpnI 1682 GGTACC GGTAC^C MvnI 1322 CGCG CG^CG NarI 2427 GGCGCC GG^CGCC NdeI 312 CATATG CA^TATG PacI 3361 TTAATTAA TTAAT^TAA PaeR7I 2938 CTCGAG C^TCGAG PshAI 1253 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 2661 GAGCTC GAGCT^C ScaI 2072 AGTACT AGT^ACT SexAI 2332 ACCTGGT A^CC[AT]GGT SfoI 2427 GGCGCC GGC^GCC SgrAI 989 CGCCGGTG C[GA]^CCGG[CT]G StuI 2682 AGGCCT AGG^CCT TaqI 2939 TCGA T^CGA TthHB8I 2939 TCGA T^CGA XhoI 2938 CTCGAG C^TCGAG XmnI 339 GAAGAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AseI 3360 ATTAAT AT^TAAT 3364 ATTAAT AsnI 3360 ATTAAT AT^TAAT 3364 ATTAAT BalI 1598 TGGCCA TGG^CCA 1606 TGGCCA BbsI 474 GAAGAC GAAGAC(2/6) 2975 GTCTTC BglII 2353 AGATCT A^GATCT 2715 AGATCT BlpI 639 GCTCAGC GC^T[ACGT]AGC 2028 GCTCAGC Bpu1102I 639 GCTCAGC GC^T[ACGT]AGC 2028 GCTCAGC BpuAI 474 GAAGAC GAAGAC(2/6) 2975 GTCTTC BsrFI 990 GCCGGT [GA]^CCGG[CT] 1200 ACCGGC BstXI 1384 CCACGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1723 CCACCCAGGTGG BstYI 2353 AGATCT [GA]^GATC[CT] 2715 AGATCT CelII 639 GCTCAGC GC^T[ACGT]AGC 2028 GCTCAGC Cfr10I 990 GCCGGT [GA]^CCGG[CT] 1200 ACCGGC HaeII 2241 GGCGCT [GA]GCGC^[CT] 2427 GGCGCC MflI 2353 AGATCT [GA]^GATC[CT] 2715 AGATCT MluNI 1598 TGGCCA TGG^CCA 1606 TGGCCA MscI 1598 TGGCCA TGG^CCA 1606 TGGCCA NheI 2190 GCTAGC G^CTAGC 3045 GCTAGC PpuMI 996 GGGACCC [GA]G^G[AT]CC[CT] 1452 AGGTCCT PshBI 3360 ATTAAT AT^TAAT 3364 ATTAAT SapI 339 GAAGAGC GCTCTTC(1/4) 2376 GAAGAGC SphI 1004 GCATGC GCATG^C 1331 GCATGC SspI 3253 AATATT AAT^ATT 3456 AATATT XcmI 562 CCATGGCCTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1517 CCAGGGCTACGATGG XhoII 2353 AGATCT [GA]^GATC[CT] 2715 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I