##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AseI AsnI AviII AvrII
BbeI BbrPI BcgI BciVI BclI
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaWI BseAI BsiWI BsmBI BspEI
BsrDI BssSI Bst1107I BstBI BstZ17I
CelII CpoI DraI EcoNI EcoRV
FbaI FspI HincII HindII HpaI
KasI MamI MfeI MluI MroI
MunI NarI NdeI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 1324 GGTACC G^GTACC
AcyI 1053 GACGCC G[GA]^CG[CT]C
AflII 227 CTTAAG C^TTAAG
AflIII 3088 ACATGT A^C[GA][CT]GT
AhdI 33 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 48 GTGCAC G^TGCAC
Aor51HI 484 AGCGCT AGC^GCT
ApaLI 48 GTGCAC G^TGCAC
Asp700I 1340 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1324 GGTACC G^GTACC
AspEI 33 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1519 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1087 GGATCC G^GATCC
BbvCI 782 CCTCAGC CCTCAGC(-5/-2)
BfrI 227 CTTAAG C^TTAAG
BsaHI 1053 GACGCC G[GA]^CG[CT]C
BsaI 737 GAGACC GGTCTC(1/5)
BsgI 2930 CTGCAC GTGCAG(16/14)
BsiEI 1286 CGGCCG CG[GA][CT]^CG
Bsp1407I 847 TGTACA T^GTACA
BspDI 20 ATCGAT AT^CGAT
BspLU11I 3088 ACATGT A^CATGT
BsrBI 1157 GAGCGG CCGCTC(-3/-3)
BsrGI 847 TGTACA T^GTACA
BssHII 114 GCGCGC G^CGCGC
BstAPI 2576 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 308 GGTGACC G^GT[ACGT]ACC
BstPI 308 GGTGACC G^GT[ACGT]ACC
ClaI 20 ATCGAT AT^CGAT
DrdI 1137 GACCTCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1286 CGGCCG C^GGCCG
Eam1105I 33 GACGGCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1286 CGGCCG C^GGCCG
Eco47III 484 AGCGCT AGC^GCT
Eco52I 1286 CGGCCG C^GGCCG
EcoO65I 308 GGTGACC G^GT[ACGT]ACC
EcoT22I 1911 ATGCAT ATGCA^T
FseI 2634 GGCCGGCC GGCCGG^CC
HaeII 484 AGCGCT [GA]GCGC^[CT]
Hin1I 1053 GACGCC G[GA]^CG[CT]C
HindIII 224 AAGCTT A^AGCTT
KpnI 1324 GGTACC GGTAC^C
MaeII 1292 ACGT A^CGT
NaeI 2635 GCCGGC GCC^GGC
NcoI 1805 CCATGG C^CATGG
NgoMIV 2635 GCCGGC G^CCGGC
NheI 95 GCTAGC G^CTAGC
NsiI 1911 ATGCAT ATGCA^T
PflFI 1519 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1557 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfiI 1013 GGCCAAGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1788 CCCGGG CCC^GGG
SpeI 1458 ACTAGT A^CTAGT
SphI 1909 GCATGC GCATG^C
SspBI 847 TGTACA T^GTACA
SspI 3891 AATATT AAT^ATT
Tth111I 1519 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 1788 CCCGGG C^CCGGG
XmaI 1788 CCCGGG C^CCGGG
XmnI 1340 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 132 GTCTAC GT^[AC][GT]AC
3675 GTCTAC
AlwNI 1576 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2489 CAGGGTCTG
AocI 3266 CCTCAGG CC^T[ACGT]AGG
3753 CCTGAGG
AspHI 48 GTGCAC G[AT]GC[AT]^C
1624 GAGCAC
AvaI 1383 CCCGAG C^[CT]CG[GA]G
1788 CCCGGG
BanII 1368 GAGCCC G[GA]GC[CT]^C
2058 GAGCCC
BbsI 1554 GAAGAC GAAGAC(2/6)
2909 GTCTTC
BglII 212 AGATCT A^GATCT
1489 AGATCT
BpuAI 1554 GAAGAC GAAGAC(2/6)
2909 GTCTTC
BsiHKAI 48 GTGCAC G[AT]GC[AT]^C
1624 GAGCAC
BsoBI 1383 CCCGAG C^[CT]CG[GA]G
1788 CCCGGG
BspHI 1060 TCATGA T^CATGA
1929 TCATGA
BspMI 793 ACCTGC ACCTGC(4/8)
1327 ACCTGC
BsrFI 2499 GCCGGT [GA]^CCGG[CT]
2635 GCCGGC
Bsu36I 3266 CCTCAGG CC^T[ACGT]AGG
3753 CCTGAGG
Cfr10I 2499 GCCGGT [GA]^CCGG[CT]
2635 GCCGGC
Eco81I 3266 CCTCAGG CC^T[ACGT]AGG
3753 CCTGAGG
EcoRI 239 GAATTC G^AATTC
912 GAATTC
KspI 529 CCGCGG CCGC^GG
1130 CCGCGG
NspI 1909 GCATGC [GA]CATG^[CT]
3088 ACATGT
PflMI 548 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCACCACCTGG
PpuMI 1135 GGGACCT [GA]G^G[AT]CC[CT]
3794 GGGACCT
RcaI 1060 TCATGA T^CATGA
1929 TCATGA
SacII 529 CCGCGG CCGC^GG
1130 CCGCGG
SmlI 227 CTTAAG C^T[CT][GA]AG
2935 CTTGAG
StuI 715 AGGCCT AGG^CCT
779 AGGCCT
TfiI 442 GAATC G^A[AT]TC
628 GATTC
Van91I 548 CCAGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1091 CCACCACCTGG
XbaI 2395 TCTAGA T^CTAGA
2469 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BalI BanI
BbvI BcnI BfaI BglI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII