##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AccI AccIII AgeI Aor51HI ApaI
AscI AviII AvrII BglI BlnI
BseAI BsiWI BsmI BspDI BspEI
BsrBI BsrDI Bst1107I BstZ17I ClaI
CpoI DraI Eco47III EcoNI EcoRV
FseI FspI KspI MfeI MroI
MunI NotI PacI PaeR7I PinAI
PmeI PshAI PvuI RsrII SacII
SalI SbfI SexAI SfiI SnaBI
SphI Sse8387I SspI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 68 enzymes) #
###################################
AatII 448 GACGTC GACGT^C
AclI 2250 AACGTT AA^CGTT
AflII 2787 CTTAAG C^TTAAG
AhdI 101 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1923 GTGCAC G^TGCAC
ApaLI 1923 GTGCAC G^TGCAC
AseI 3043 ATTAAT AT^TAAT
AsnI 3043 ATTAAT AT^TAAT
AspEI 101 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1488 CACGTG CAC^GTG
BbsI 2925 GAAGAC GAAGAC(2/6)
BbvCI 825 CCTCAGC CCTCAGC(-5/-2)
BcgI 9 CGAGCTAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 4141 TGATCA T^GATCA
BfrI 2787 CTTAAG C^TTAAG
BlpI 1633 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1633 GCTAAGC GC^T[ACGT]AGC
BpuAI 2925 GAAGAC GAAGAC(2/6)
BsaAI 1488 CACGTG [CT]AC^GT[GA]
BsaBI 1669 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1024 GAGACC GGTCTC(1/5)
BsaWI 1936 TCCGGT [AT]^CCGG[AT]
BsiEI 952 CGGCCG CG[GA][CT]^CG
BsmBI 2346 CGTCTC CGTCTC(1/5)
Bsp1407I 3917 TGTACA T^GTACA
BspHI 2607 TCATGA T^CATGA
BsrFI 2341 GCCGGC [GA]^CCGG[CT]
BsrGI 3917 TGTACA T^GTACA
BssSI 1183 CTCGTG CACGAG(-5/-1)
BstAPI 1538 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3727 TTCGAA TT^CGAA
BstXI 1178 CCATGCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1633 GCTAAGC GC^T[ACGT]AGC
Cfr10I 2341 GCCGGC [GA]^CCGG[CT]
DraIII 3337 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2152 GACAACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 952 CGGCCG C^GGCCG
Eam1105I 101 GACGAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 952 CGGCCG C^GGCCG
Eco52I 952 CGGCCG C^GGCCG
EcoRI 1290 GAATTC G^AATTC
EcoT22I 3765 ATGCAT ATGCA^T
FbaI 4141 TGATCA T^GATCA
HincII 4479 GTTAAC GT[CT]^[GA]AC
HindII 4479 GTTAAC GT[CT]^[GA]AC
HindIII 2294 AAGCTT A^AGCTT
HpaI 4479 GTTAAC GTT^AAC
MamI 1669 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 335 ACGCGT A^CGCGT
NaeI 2341 GCCGGC GCC^GGC
NdeI 4266 CATATG CA^TATG
NgoMIV 2341 GCCGGC G^CCGGC
NheI 262 GCTAGC G^CTAGC
NruI 1045 TCGCGA TCG^CGA
NsiI 3765 ATGCAT ATGCA^T
NspV 3727 TTCGAA TT^CGAA
PmaCI 1488 CACGTG CAC^GTG
PmlI 1488 CACGTG CAC^GTG
PshBI 3043 ATTAAT AT^TAAT
Psp1406I 2250 AACGTT AA^CGTT
RcaI 2607 TCATGA T^CATGA
SacI 3835 GAGCTC GAGCT^C
SapI 4234 GCTCTTC GCTCTTC(1/4)
ScaI 2180 AGTACT AGT^ACT
SfuI 3727 TTCGAA TT^CGAA
SgrAI 2340 CGCCGGCG C[GA]^CCGG[CT]G
SpeI 2587 ACTAGT A^CTAGT
SspBI 3917 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 1828 GGTACC G^GTACC
2716 GGTACC
Asp718I 1828 GGTACC G^GTACC
2716 GGTACC
AspI 2611 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2928 GACAGAGTC
BalI 648 TGGCCA TGG^CCA
902 TGGCCA
BamHI 1428 GGATCC G^GATCC
3352 GGATCC
BciVI 3408 GGATAC GTATCC(6/5)
4211 GTATCC
BglII 466 AGATCT A^GATCT
1316 AGATCT
BspLU11I 1906 ACATGT A^CATGT
4078 ACATGT
BssHII 218 GCGCGC G^CGCGC
597 GCGCGC
BstEII 1721 GGTCACC G^GT[ACGT]ACC
3143 GGTCACC
BstPI 1721 GGTCACC G^GT[ACGT]ACC
3143 GGTCACC
EcoO65I 1721 GGTCACC G^GT[ACGT]ACC
3143 GGTCACC
KpnI 1828 GGTACC GGTAC^C
2716 GGTACC
MluNI 648 TGGCCA TGG^CCA
902 TGGCCA
MscI 648 TGGCCA TGG^CCA
902 TGGCCA
PflFI 2611 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2928 GACAGAGTC
PvuII 1989 CAGCTG CAG^CTG
4194 CAGCTG
SmaI 603 CCCGGG CCC^GGG
730 CCCGGG
SmlI 2106 CTCAAG C^T[CT][GA]AG
2787 CTTAAG
Tth111I 2611 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2928 GACAGAGTC
XmaCI 603 CCCGGG C^CCGGG
730 CCCGGG
XmaI 603 CCCGGG C^CCGGG
730 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI