##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
Alw44I Aor51HI ApaLI AscI Asp700I
AspI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BcnI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BspEI BsrFI BssHII BssSI
BstBI BstEII BstPI CelII Cfr10I
CpoI DraIII Eco47III EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FseI FspI
HaeII HapII Hin1I HincII HindII
HpaI HpaII KasI MamI MluNI
MroI MscI MspI NaeI NarI
NciI NcoI NgoMIV NruI NsiI
NspV PaeR7I PflFI PinAI PmaCI
PmeI PmlI PvuI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
StuI StyI Tth111I XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AclI 1132 AACGTT AA^CGTT
AhdI 955 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2774 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspEI 955 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 50 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3170 AGATCT A^GATCT
BsaI 3363 GAGACC GGTCTC(1/5)
BsiEI 18 CGGCCG CG[GA][CT]^CG
BspDI 1558 ATCGAT AT^CGAT
BspLU11I 4175 ACATGT A^CATGT
Bst1107I 3102 GTATAC GTA^TAC
BstAPI 1273 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1694 CCAATTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3102 GTATAC GTA^TAC
ClaI 1558 ATCGAT AT^CGAT
DrdI 3078 GACTCTGCGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 54 CCGCGG C^C[GA][CT]GG
EagI 18 CGGCCG C^GGCCG
Eam1105I 955 GACACTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 18 CGGCCG C^GGCCG
Eco52I 18 CGGCCG C^GGCCG
HindIII 564 AAGCTT A^AGCTT
KspI 54 CCGCGG CCGC^GG
MfeI 3262 CAATTG C^AATTG
MluI 1042 ACGCGT A^CGCGT
MunI 3262 CAATTG C^AATTG
NdeI 661 CATATG CA^TATG
NheI 1003 GCTAGC G^CTAGC
NotI 17 GCGGCCGC GC^GGCCGC
PacI 2516 TTAATTAA TTAAT^TAA
PflMI 1072 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 161 GGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1132 AACGTT AA^CGTT
SacII 54 CCGCGG CCGC^GG
SpeI 1482 ACTAGT A^CTAGT
SwaI 2704 ATTTAAAT ATTT^AAAT
TaqI 1559 TCGA T^CGA
TthHB8I 1559 TCGA T^CGA
Van91I 1072 CCAAAGATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 713 GGTACC G^GTACC
1704 GGTACC
AccI 1996 GTAGAC GT^[AC][GT]AC
3102 GTATAC
AlwI 471 GATCC GGATC(4/5)
978 GATCC
AocI 150 CCTAAGG CC^T[ACGT]AGG
1792 CCTGAGG
ApaI 48 GGGCCC GGGCC^C
2037 GGGCCC
Asp718I 713 GGTACC G^GTACC
1704 GGTACC
AvaI 836 CCCGAG C^[CT]CG[GA]G
1507 CCCGAG
BanI 713 GGTACC G^G[CT][GA]CC
1704 GGTACC
BciVI 765 GTATCC GTATCC(6/5)
2978 GTATCC
BseRI 1780 GAGGAG GAGGAG(10/8)
1969 GAGGAG
BsmI 1866 GAATGC GAATGC(1/-1)
76 GCATTC
BsoBI 836 CCCGAG C^[CT]CG[GA]G
1507 CCCGAG
Bsp1407I 734 TGTACA T^GTACA
3058 TGTACA
BsrBI 21 CCGCTC CCGCTC(-3/-3)
15 GAGCGG
BsrDI 842 CATTGC GCAATG(2/0)
2079 GCAATG
BsrGI 734 TGTACA T^GTACA
3058 TGTACA
Bsu36I 150 CCTAAGG CC^T[ACGT]AGG
1792 CCTGAGG
EaeI 18 CGGCCG [CT]^GGCC[GA]
1069 CGGCCA
Eco81I 150 CCTAAGG CC^T[ACGT]AGG
1792 CCTGAGG
FauI 53 CCCGC CCCGC(4/6)
10 GCGGG
HgaI 1849 GACGC GACGC(5/10)
1906 GCGTC
KpnI 713 GGTACC GGTAC^C
1704 GGTACC
MaeII 1133 ACGT A^CGT
3756 ACGT
MslI 476 CATTCTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4328 CATGAATGTG
PshAI 896 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2851 GACTGTCGTC
SacI 1197 GAGCTC GAGCT^C
1638 GAGCTC
SapI 1219 GAAGAGC GCTCTTC(1/4)
1878 GAAGAGC
SspBI 734 TGTACA T^GTACA
3058 TGTACA
SspI 2348 AATATT AAT^ATT
3244 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI AseI AsnI AspHI
AvaII BanII BbsI BbvI BclI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspHI BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRI
EcoRII FbaI Fnu4HI FokI HaeIII
HhaI HinP1I HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MvnI MwoI NdeII NlaIII NlaIV
NspI PleI PshBI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XbaI XhoII