##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Aor51HI ApaI AscI Asp718I
AspEI AviII BanI BbeI BbrPI
BbvCI BcgI BglI BsaAI BsaBI
BsaHI BsaI BsiEI BsiWI BsmBI
BspDI BssHII BssSI BstBI BstEII
BstPI ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FseI FspI Hin1I KasI
KpnI KspI MamI MfeI MluI
MunI NarI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 130 TCCGGA T^CCGGA
AflII 2170 CTTAAG C^TTAAG
AflIII 2472 ACATGT A^C[GA][CT]GT
Alw44I 2571 GTGCAC G^TGCAC
ApaLI 2571 GTGCAC G^TGCAC
Asp700I 2328 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 3431 CCTAGG C^CTAGG
BamHI 983 GGATCC G^GATCC
BciVI 4214 GGATAC GTATCC(6/5)
BclI 1457 TGATCA T^GATCA
BfrI 2170 CTTAAG C^TTAAG
BglII 3112 AGATCT A^GATCT
BlnI 3431 CCTAGG C^CTAGG
BlpI 3718 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3718 GCTGAGC GC^T[ACGT]AGC
BseAI 130 TCCGGA T^CCGGA
BsmI 400 GCATTC GAATGC(1/-1)
BspEI 130 TCCGGA T^CCGGA
BspLU11I 2472 ACATGT A^CATGT
BsrBI 3697 GAGCGG CCGCTC(-3/-3)
BsrDI 2037 GCAATG GCAATG(2/0)
BsrFI 3731 GCCGGC [GA]^CCGG[CT]
CelII 3718 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3731 GCCGGC [GA]^CCGG[CT]
DraIII 261 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 676 GACGCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 102 CCACGG C^C[GA][CT]GG
EcoRI 4105 GAATTC G^AATTC
FbaI 1457 TGATCA T^GATCA
HaeII 159 GGCGCT [GA]GCGC^[CT]
MroI 130 TCCGGA T^CCGGA
NaeI 3731 GCCGGC GCC^GGC
NgoMIV 3731 GCCGGC G^CCGGC
PflMI 1549 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 3721 GAGCTC GAGCT^C
SphI 2151 GCATGC GCATG^C
StuI 1344 AGGCCT AGG^CCT
Van91I 1549 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3203 TCTAGA T^CTAGA
XcmI 1545 CCACCCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2328 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2969 GTATAC GT^[AC][GT]AC
4159 GTATAC
AocI 668 CCTCAGG CC^T[ACGT]AGG
2744 CCTTAGG
AseI 1285 ATTAAT AT^TAAT
4023 ATTAAT
AsnI 1285 ATTAAT AT^TAAT
4023 ATTAAT
AspI 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACACAGTC
BalI 323 TGGCCA TGG^CCA
1078 TGGCCA
BsaWI 130 TCCGGA [AT]^CCGG[AT]
3489 ACCGGA
Bst1107I 2969 GTATAC GTA^TAC
4159 GTATAC
BstXI 1097 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2600 CCAAACCACTGG
BstZ17I 2969 GTATAC GTA^TAC
4159 GTATAC
Bsu36I 668 CCTCAGG CC^T[ACGT]AGG
2744 CCTTAGG
EaeI 323 TGGCCA [CT]^GGCC[GA]
1078 TGGCCA
Eco81I 668 CCTCAGG CC^T[ACGT]AGG
2744 CCTTAGG
EcoNI 1626 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3431 CCTAGGTGAGG
FauI 1051 GCGGG CCCGC(4/6)
3699 GCGGG
HgaI 676 GACGC GACGC(5/10)
700 GCGTC
MaeII 123 ACGT A^CGT
1925 ACGT
MluNI 323 TGGCCA TGG^CCA
1078 TGGCCA
MscI 323 TGGCCA TGG^CCA
1078 TGGCCA
PflFI 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACACAGTC
PpuMI 601 GGGTCCC [GA]G^G[AT]CC[CT]
3560 AGGTCCT
PshBI 1285 ATTAAT AT^TAAT
4023 ATTAAT
PstI 2189 CTGCAG CTGCA^G
3400 CTGCAG
PvuII 1797 CAGCTG CAG^CTG
1852 CAGCTG
SspI 3012 AATATT AAT^ATT
3069 AATATT
Tth111I 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1885 GACACAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspHI BspMI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EarI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RcaI RsaI Sau3AI Sau96I ScaI
ScrFI SexAI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII