##################################
# NO CUT ENZYMES (   85 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Aor51HI     ApaI        AscI        Asp718I     
AspEI       AviII       BanI        BbeI        BbrPI       
BbvCI       BcgI        BglI        BsaAI       BsaBI       
BsaHI       BsaI        BsiEI       BsiWI       BsmBI       
BspDI       BssHII      BssSI       BstBI       BstEII      
BstPI       ClaI        CpoI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
EcoT22I     FseI        FspI        Hin1I       KasI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NarI        NcoI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
Sse8387I    SwaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AccIII        130  TCCGGA               T^CCGGA
AflII        2170  CTTAAG               C^TTAAG
AflIII       2472  ACATGT               A^C[GA][CT]GT
Alw44I       2571  GTGCAC               G^TGCAC
ApaLI        2571  GTGCAC               G^TGCAC
Asp700I      2328  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII        3431  CCTAGG               C^CTAGG
BamHI         983  GGATCC               G^GATCC
BciVI        4214  GGATAC               GTATCC(6/5)
BclI         1457  TGATCA               T^GATCA
BfrI         2170  CTTAAG               C^TTAAG
BglII        3112  AGATCT               A^GATCT
BlnI         3431  CCTAGG               C^CTAGG
BlpI         3718  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     3718  GCTGAGC              GC^T[ACGT]AGC
BseAI         130  TCCGGA               T^CCGGA
BsmI          400  GCATTC               GAATGC(1/-1)
BspEI         130  TCCGGA               T^CCGGA
BspLU11I     2472  ACATGT               A^CATGT
BsrBI        3697  GAGCGG               CCGCTC(-3/-3)
BsrDI        2037  GCAATG               GCAATG(2/0)
BsrFI        3731  GCCGGC               [GA]^CCGG[CT]
CelII        3718  GCTGAGC              GC^T[ACGT]AGC
Cfr10I       3731  GCCGGC               [GA]^CCGG[CT]
DraIII        261  CACGGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          676  GACGCAGCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          102  CCACGG               C^C[GA][CT]GG
EcoRI        4105  GAATTC               G^AATTC
FbaI         1457  TGATCA               T^GATCA
HaeII         159  GGCGCT               [GA]GCGC^[CT]
MroI          130  TCCGGA               T^CCGGA
NaeI         3731  GCCGGC               GCC^GGC
NgoMIV       3731  GCCGGC               G^CCGGC
PflMI        1549  CCAAATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI         3721  GAGCTC               GAGCT^C
SphI         2151  GCATGC               GCATG^C
StuI         1344  AGGCCT               AGG^CCT
Van91I       1549  CCAAATACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         3203  TCTAGA               T^CTAGA
XcmI         1545  CCACCCAAATACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI         2328  GAATGGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI         2969  GTATAC               GT^[AC][GT]AC
             4159  GTATAC              
AocI          668  CCTCAGG              CC^T[ACGT]AGG
             2744  CCTTAGG             
AseI         1285  ATTAAT               AT^TAAT
             4023  ATTAAT              
AsnI         1285  ATTAAT               AT^TAAT
             4023  ATTAAT              
AspI          210  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1885  GACACAGTC           
BalI          323  TGGCCA               TGG^CCA
             1078  TGGCCA              
BsaWI         130  TCCGGA               [AT]^CCGG[AT]
             3489  ACCGGA              
Bst1107I     2969  GTATAC               GTA^TAC
             4159  GTATAC              
BstXI        1097  CCAGGACTTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2600  CCAAACCACTGG        
BstZ17I      2969  GTATAC               GTA^TAC
             4159  GTATAC              
Bsu36I        668  CCTCAGG              CC^T[ACGT]AGG
             2744  CCTTAGG             
EaeI          323  TGGCCA               [CT]^GGCC[GA]
             1078  TGGCCA              
Eco81I        668  CCTCAGG              CC^T[ACGT]AGG
             2744  CCTTAGG             
EcoNI        1626  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3431  CCTAGGTGAGG         
FauI         1051  GCGGG                CCCGC(4/6)
             3699  GCGGG               
HgaI          676  GACGC                GACGC(5/10)
              700  GCGTC               
MaeII         123  ACGT                 A^CGT
             1925  ACGT                
MluNI         323  TGGCCA               TGG^CCA
             1078  TGGCCA              
MscI          323  TGGCCA               TGG^CCA
             1078  TGGCCA              
PflFI         210  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1885  GACACAGTC           
PpuMI         601  GGGTCCC              [GA]G^G[AT]CC[CT]
             3560  AGGTCCT             
PshBI        1285  ATTAAT               AT^TAAT
             4023  ATTAAT              
PstI         2189  CTGCAG               CTGCA^G
             3400  CTGCAG              
PvuII        1797  CAGCTG               CAG^CTG
             1852  CAGCTG              
SspI         3012  AATATT               AAT^ATT
             3069  AATATT              
Tth111I       210  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1885  GACACAGTC           


#########################################
# 3 OR MORE CUT ENZYMES (  106 enzymes) #
#########################################

AccII       AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AspHI       AvaI        
AvaII       BanII       BbsI        BbvI        BcnI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    Bsp1407I    
BspHI       BspMI       BsrGI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DraII       EarI        EcoO109I    EcoRII      
EcoT14I     Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HincII      HindII      HindIII     
HinfI       HpaI        HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PleI        
RcaI        RsaI        Sau3AI      Sau96I      ScaI        
ScrFI       SexAI       SfaNI       SfcI        SmlI        
SspBI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII