################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI Aor51HI ApaI AscI Asp718I AspEI AviII BanI BbeI BbrPI BbvCI BcgI BglI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI BsmBI BspDI BssHII BssSI BstBI BstEII BstPI ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FseI FspI Hin1I KasI KpnI KspI MamI MfeI MluI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 130 TCCGGA T^CCGGA AflII 2170 CTTAAG C^TTAAG AflIII 2472 ACATGT A^C[GA][CT]GT Alw44I 2571 GTGCAC G^TGCAC ApaLI 2571 GTGCAC G^TGCAC Asp700I 2328 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 3431 CCTAGG C^CTAGG BamHI 983 GGATCC G^GATCC BciVI 4214 GGATAC GTATCC(6/5) BclI 1457 TGATCA T^GATCA BfrI 2170 CTTAAG C^TTAAG BglII 3112 AGATCT A^GATCT BlnI 3431 CCTAGG C^CTAGG BlpI 3718 GCTGAGC GC^T[ACGT]AGC Bpu1102I 3718 GCTGAGC GC^T[ACGT]AGC BseAI 130 TCCGGA T^CCGGA BsmI 400 GCATTC GAATGC(1/-1) BspEI 130 TCCGGA T^CCGGA BspLU11I 2472 ACATGT A^CATGT BsrBI 3697 GAGCGG CCGCTC(-3/-3) BsrDI 2037 GCAATG GCAATG(2/0) BsrFI 3731 GCCGGC [GA]^CCGG[CT] CelII 3718 GCTGAGC GC^T[ACGT]AGC Cfr10I 3731 GCCGGC [GA]^CCGG[CT] DraIII 261 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 676 GACGCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 102 CCACGG C^C[GA][CT]GG EcoRI 4105 GAATTC G^AATTC FbaI 1457 TGATCA T^GATCA HaeII 159 GGCGCT [GA]GCGC^[CT] MroI 130 TCCGGA T^CCGGA NaeI 3731 GCCGGC GCC^GGC NgoMIV 3731 GCCGGC G^CCGGC PflMI 1549 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SacI 3721 GAGCTC GAGCT^C SphI 2151 GCATGC GCATG^C StuI 1344 AGGCCT AGG^CCT Van91I 1549 CCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3203 TCTAGA T^CTAGA XcmI 1545 CCACCCAAATACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2328 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2969 GTATAC GT^[AC][GT]AC 4159 GTATAC AocI 668 CCTCAGG CC^T[ACGT]AGG 2744 CCTTAGG AseI 1285 ATTAAT AT^TAAT 4023 ATTAAT AsnI 1285 ATTAAT AT^TAAT 4023 ATTAAT AspI 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1885 GACACAGTC BalI 323 TGGCCA TGG^CCA 1078 TGGCCA BsaWI 130 TCCGGA [AT]^CCGG[AT] 3489 ACCGGA Bst1107I 2969 GTATAC GTA^TAC 4159 GTATAC BstXI 1097 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2600 CCAAACCACTGG BstZ17I 2969 GTATAC GTA^TAC 4159 GTATAC Bsu36I 668 CCTCAGG CC^T[ACGT]AGG 2744 CCTTAGG EaeI 323 TGGCCA [CT]^GGCC[GA] 1078 TGGCCA Eco81I 668 CCTCAGG CC^T[ACGT]AGG 2744 CCTTAGG EcoNI 1626 CCTAGAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3431 CCTAGGTGAGG FauI 1051 GCGGG CCCGC(4/6) 3699 GCGGG HgaI 676 GACGC GACGC(5/10) 700 GCGTC MaeII 123 ACGT A^CGT 1925 ACGT MluNI 323 TGGCCA TGG^CCA 1078 TGGCCA MscI 323 TGGCCA TGG^CCA 1078 TGGCCA PflFI 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1885 GACACAGTC PpuMI 601 GGGTCCC [GA]G^G[AT]CC[CT] 3560 AGGTCCT PshBI 1285 ATTAAT AT^TAAT 4023 ATTAAT PstI 2189 CTGCAG CTGCA^G 3400 CTGCAG PvuII 1797 CAGCTG CAG^CTG 1852 CAGCTG SspI 3012 AATATT AAT^ATT 3069 AATATT Tth111I 210 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC 1885 GACACAGTC ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspHI BspMI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RcaI RsaI Sau3AI Sau96I ScaI ScrFI SexAI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII