################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AccIII Alw44I ApaLI AscI Asp718I AvrII BamHI BbeI BbrPI BcgI BglI BlnI BlpI Bpu1102I BsaAI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoO65I EcoRV FseI HincII HindII HpaI KasI KpnI MfeI MluI MroI MunI NarI NcoI NdeI NotI NruI PacI PaeR7I PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccI 294 GTAGAC GT^[AC][GT]AC AclI 723 AACGTT AA^CGTT AflII 2288 CTTAAG C^TTAAG AflIII 1928 ACACGT A^C[GA][CT]GT AgeI 678 ACCGGT A^CCGGT AocI 1879 CCTAAGG CC^T[ACGT]AGG ApaI 3710 GGGCCC GGGCC^C AspI 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BclI 2746 TGATCA T^GATCA BfrI 2288 CTTAAG C^TTAAG BglII 4060 AGATCT A^GATCT BsaI 3071 GAGACC GGTCTC(1/5) BsaWI 678 ACCGGT [AT]^CCGG[AT] BsrDI 2900 GCAATG GCAATG(2/0) BssHII 34 GCGCGC G^CGCGC BssSI 1182 CACGAG CACGAG(-5/-1) BstAPI 2641 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1879 CCTAAGG CC^T[ACGT]AGG Eco81I 1879 CCTAAGG CC^T[ACGT]AGG EcoNI 1108 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1294 GAATTC G^AATTC EcoT22I 4043 ATGCAT ATGCA^T FbaI 2746 TGATCA T^GATCA HindIII 1439 AAGCTT A^AGCTT KspI 309 CCGCGG CCGC^GG NaeI 283 GCCGGC GCC^GGC NgoMIV 283 GCCGGC G^CCGGC NsiI 4043 ATGCAT ATGCA^T PflFI 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 739 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 678 ACCGGT A^CCGGT Psp1406I 723 AACGTT AA^CGTT SacII 309 CCGCGG CCGC^GG SphI 1545 GCATGC GCATG^C Tth111I 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 739 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AcyI 254 GACGCC G[GA]^CG[CT]C 639 GACGCC AhdI 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3471 GACTGCAAGTC AseI 3361 ATTAAT AT^TAAT 3915 ATTAAT AsnI 3361 ATTAAT AT^TAAT 3915 ATTAAT AspEI 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3471 GACTGCAAGTC AviII 1072 TGCGCA TGC^GCA 1935 TGCGCA BalI 2550 TGGCCA TGG^CCA 3400 TGGCCA BanI 1628 GGCACC G^G[CT][GA]CC 3284 GGTGCC BbvCI 194 CCTCAGC CCTCAGC(-5/-2) 2037 CCTCAGC BciVI 1723 GGATAC GTATCC(6/5) 3822 GGATAC BsaBI 806 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3293 GATTTCCATC BsaHI 254 GACGCC G[GA]^CG[CT]C 639 GACGCC BsmBI 204 CGTCTC CGTCTC(1/5) 1256 CGTCTC BsmI 1381 GAATGC GAATGC(1/-1) 4041 GAATGC DrdI 111 GACTCTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1171 GACAGCTGAGTC DsaI 309 CCGCGG C^C[GA][CT]GG 489 CCGTGG Eam1105I 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3471 GACTGCAAGTC FspI 1072 TGCGCA TGC^GCA 1935 TGCGCA Hin1I 254 GACGCC G[GA]^CG[CT]C 639 GACGCC MamI 806 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3293 GATTTCCATC MluNI 2550 TGGCCA TGG^CCA 3400 TGGCCA MscI 2550 TGGCCA TGG^CCA 3400 TGGCCA NheI 568 GCTAGC G^CTAGC 884 GCTAGC PshBI 3361 ATTAAT AT^TAAT 3915 ATTAAT ScaI 1970 AGTACT AGT^ACT 3187 AGTACT SmaI 91 CCCGGG CCC^GGG 1632 CCCGGG SspI 3912 AATATT AAT^ATT 4191 AATATT StuI 2601 AGGCCT AGG^CCT 3371 AGGCCT XcmI 894 CCAGTGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4086 CCAGGGCGATGTTGG XmaCI 91 CCCGGG C^CCGGG 1632 CCCGGG XmaI 91 CCCGGG C^CCGGG 1632 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI Aor51HI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstBI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI Eco47III EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI