##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII Alw44I ApaLI
AscI Asp718I AvrII BamHI BbeI
BbrPI BcgI BglI BlnI BlpI
Bpu1102I BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraI
DraIII EagI EclXI Eco52I EcoO65I
EcoRV FseI HincII HindII HpaI
KasI KpnI MfeI MluI MroI
MunI NarI NcoI NdeI NotI
NruI PacI PaeR7I PmaCI PmeI
PmlI PshAI PvuI RcaI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfoI SgrAI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AccI 294 GTAGAC GT^[AC][GT]AC
AclI 723 AACGTT AA^CGTT
AflII 2288 CTTAAG C^TTAAG
AflIII 1928 ACACGT A^C[GA][CT]GT
AgeI 678 ACCGGT A^CCGGT
AocI 1879 CCTAAGG CC^T[ACGT]AGG
ApaI 3710 GGGCCC GGGCC^C
AspI 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 2746 TGATCA T^GATCA
BfrI 2288 CTTAAG C^TTAAG
BglII 4060 AGATCT A^GATCT
BsaI 3071 GAGACC GGTCTC(1/5)
BsaWI 678 ACCGGT [AT]^CCGG[AT]
BsrDI 2900 GCAATG GCAATG(2/0)
BssHII 34 GCGCGC G^CGCGC
BssSI 1182 CACGAG CACGAG(-5/-1)
BstAPI 2641 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1879 CCTAAGG CC^T[ACGT]AGG
Eco81I 1879 CCTAAGG CC^T[ACGT]AGG
EcoNI 1108 CCTCCTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1294 GAATTC G^AATTC
EcoT22I 4043 ATGCAT ATGCA^T
FbaI 2746 TGATCA T^GATCA
HindIII 1439 AAGCTT A^AGCTT
KspI 309 CCGCGG CCGC^GG
NaeI 283 GCCGGC GCC^GGC
NgoMIV 283 GCCGGC G^CCGGC
NsiI 4043 ATGCAT ATGCA^T
PflFI 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 739 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 678 ACCGGT A^CCGGT
Psp1406I 723 AACGTT AA^CGTT
SacII 309 CCGCGG CCGC^GG
SphI 1545 GCATGC GCATG^C
Tth111I 3422 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 739 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 254 GACGCC G[GA]^CG[CT]C
639 GACGCC
AhdI 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3471 GACTGCAAGTC
AseI 3361 ATTAAT AT^TAAT
3915 ATTAAT
AsnI 3361 ATTAAT AT^TAAT
3915 ATTAAT
AspEI 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3471 GACTGCAAGTC
AviII 1072 TGCGCA TGC^GCA
1935 TGCGCA
BalI 2550 TGGCCA TGG^CCA
3400 TGGCCA
BanI 1628 GGCACC G^G[CT][GA]CC
3284 GGTGCC
BbvCI 194 CCTCAGC CCTCAGC(-5/-2)
2037 CCTCAGC
BciVI 1723 GGATAC GTATCC(6/5)
3822 GGATAC
BsaBI 806 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3293 GATTTCCATC
BsaHI 254 GACGCC G[GA]^CG[CT]C
639 GACGCC
BsmBI 204 CGTCTC CGTCTC(1/5)
1256 CGTCTC
BsmI 1381 GAATGC GAATGC(1/-1)
4041 GAATGC
DrdI 111 GACTCTCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1171 GACAGCTGAGTC
DsaI 309 CCGCGG C^C[GA][CT]GG
489 CCGTGG
Eam1105I 254 GACGCCTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3471 GACTGCAAGTC
FspI 1072 TGCGCA TGC^GCA
1935 TGCGCA
Hin1I 254 GACGCC G[GA]^CG[CT]C
639 GACGCC
MamI 806 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3293 GATTTCCATC
MluNI 2550 TGGCCA TGG^CCA
3400 TGGCCA
MscI 2550 TGGCCA TGG^CCA
3400 TGGCCA
NheI 568 GCTAGC G^CTAGC
884 GCTAGC
PshBI 3361 ATTAAT AT^TAAT
3915 ATTAAT
ScaI 1970 AGTACT AGT^ACT
3187 AGTACT
SmaI 91 CCCGGG CCC^GGG
1632 CCCGGG
SspI 3912 AATATT AAT^ATT
4191 AATATT
StuI 2601 AGGCCT AGG^CCT
3371 AGGCCT
XcmI 894 CCAGTGAAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4086 CCAGGGCGATGTTGG
XmaCI 91 CCCGGG C^CCGGG
1632 CCCGGG
XmaI 91 CCCGGG C^CCGGG
1632 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI Aor51HI ApoI Asp700I
AspHI AvaI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstBI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
Eco47III EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI NspV PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmnI